Cloning of GST and HSP90 genes of Sinonovacula constricta and analysis of their expression characteristics under ammonia nitrogen stress

Zhang Huan; Dong Yinghui; Yao Hanhan; Yu Kaijia; Hu Yueya; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2020.04.008

Volume 42 Issue 4

Nov. 2020

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Zhang Huan，Dong Yinghui，Yao Hanhan, et al. Cloning of GST and HSP90 genes of Sinonovacula constricta and analysis of their expression characteristics under ammonia nitrogen stress[J]. Haiyang Xuebao，2020, 42(4)：66–78，doi:10.3969/j.issn.0253−4193.2020.04.008

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Zhang Huan，Dong Yinghui，Yao Hanhan, et al. Cloning of GST and HSP90 genes of Sinonovacula constricta and analysis of their expression characteristics under ammonia nitrogen stress[J]. Haiyang Xuebao，2020, 42(4)：66–78，doi:10.3969/j.issn.0253−4193.2020.04.008

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Cloning of GST and HSP90 genes of Sinonovacula constricta and analysis of their expression characteristics under ammonia nitrogen stress

doi: 10.3969/j.issn.0253-4193.2020.04.008

Zhang Huan^{1, 2
,},
Dong Yinghui²,
Yao Hanhan²,
Yu Kaijia²,
Hu Yueya²,
Lin Zhihua^{2
,
,}

1.
School of Marine Sciences, Ningbo University, Ningbo 315823, China
2.
Zhejiang Key Laboratory of Aquatic Germplasm Resources, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China

Received Date: 2019-05-27
Rev Recd Date: 2019-08-21

Available Online: 2020-11-18

Publish Date: 2020-04-25

Abstract

Abstract

The full-length cDNA of glutathione S-transferase (Sc-GSTσ) and heat shock protein 90 (Sc-HSP90) genes were cloned from Sinonovacula constricta and their expression characteristics under ammonia nitrogen stress were analyzed. The results show that the full-length cDNA of Sc-GSTσ was 1 414 bp, and containing 639 bp open reading frame (ORF), encoding 212 amino acid polypeptides. The homology of amino acid sequence of Sc-GSTσ with other species’ GST amino acid sequence was 31.88%−43.40%. The full-length cDNA of Sc-HSP90 was 2 752 bp, ORF was 2 181 bp, encoding 726 amino acids. The amino acid sequence was 76.77%−87.05% homology with other species. Quantitative analysis showed that Sc-GSTσ and Sc-HSP90 genes were expressed in all tested tissues, the strongest expression being in the digestive gland. After exposure to ammonia, the mRNA expression of Sc-GSTσ and Sc-HSP90 were significantly up-regulated (p<0.05) in the digestive gland, indicating that ammonia stress induced stress response, both GST and HSP90 may be participate the process of detoxification or defense. However, the decrease of expression in the later period of stress is presumed to be due to the organism have limited ability to defense, which is not enough to protect the host from stress-induced cell damage.
- Sinonovacula constricta,
- GST,
- HSP90,
- ammonia nitrogen,
- expression characteristics

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References(45)

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