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Qian Baoying, Huang Hongli, Li Juan, Cao Mingyue, Zhang Yu, Zhao Yayun, Xue Liangyi. Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea[J]. Haiyang Xuebao, 2018, 40(12): 31-39. doi: 10.3969/j.issn.0253-4193.2018.12.004
Citation: Qian Baoying, Huang Hongli, Li Juan, Cao Mingyue, Zhang Yu, Zhao Yayun, Xue Liangyi. Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea[J]. Haiyang Xuebao, 2018, 40(12): 31-39. doi: 10.3969/j.issn.0253-4193.2018.12.004

Effects of fasting on the mRNA levels and enzymatic activities of hormone sensitive lipase and acetyl-CoA carboxylase in the large yellow croaker Larimichthys crocea

doi: 10.3969/j.issn.0253-4193.2018.12.004
  • Received Date: 2017-12-11
  • Rev Recd Date: 2018-01-18
  • Hormone sensitive lipase (HSL) and acetyl-CoA carboxylase (ACC) are key enzymes in lipid metabolism. Muscle and liver tissues are major main places. To study the role of the two enzymes during fasting, we detected the mRNA expression and activity changes of HSL and ACC in large yellow croaker muscle and liver tissues during 35 d fasting by real-time fluorescent quantitative PCR (qPCR) and Elisa, respectively. The results showed that fasting significantly decreased fat content and ACC mRNA level (P< 0.05), and increased HSL mRNA expression in both muscle and liver tissues (P<0.05). Moreover, fasting had a significant influence on the activities of HSL and ACC in both muscle and liver tissues of large yellow croaker (P<0.05). Muscle and liver HSL activities were moderately correlated with the corresponding mRNA expression level, as well as ACC in muscle. But liver ACC activity was negatively correlated with the mRNA level. These results suggested that the expression of HSL and ACC were regulated in both pre-translational and post-translational levels during fasting in the large yellow croaker. The results of the present study provide insights into the molecular mechanisms underlying the lipid metabolic response of the large yellow croaker to fasting.
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