Screening the diversity and activity of culturable bacteria isolated from mangrove rhizosphere soil at Shankou

Zhao Yahui; Zhang Shulin; Wu Jiafa; Huang Xinjing; Wu Shuang; Zhang Zheng; Yang Lifang; Long Han; Xuan Jincai; Jiang Mingguo

doi:10.3969/j.issn.0253-4193.2018.08.014

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Zhao Yahui, Zhang Shulin, Wu Jiafa, Huang Xinjing, Wu Shuang, Zhang Zheng, Yang Lifang, Long Han, Xuan Jincai, Jiang Mingguo. Screening the diversity and activity of culturable bacteria isolated from mangrove rhizosphere soil at Shankou[J]. Haiyang Xuebao, 2018, 40(8): 138-151. doi: 10.3969/j.issn.0253-4193.2018.08.014

Citation:

Zhao Yahui, Zhang Shulin, Wu Jiafa, Huang Xinjing, Wu Shuang, Zhang Zheng, Yang Lifang, Long Han, Xuan Jincai, Jiang Mingguo. Screening the diversity and activity of culturable bacteria isolated from mangrove rhizosphere soil at Shankou[J]. Haiyang Xuebao, 2018, 40(8): 138-151. doi: 10.3969/j.issn.0253-4193.2018.08.014

Citation:

Zhao Yahui, Zhang Shulin, Wu Jiafa, Huang Xinjing, Wu Shuang, Zhang Zheng, Yang Lifang, Long Han, Xuan Jincai, Jiang Mingguo. Screening the diversity and activity of culturable bacteria isolated from mangrove rhizosphere soil at Shankou[J]. Haiyang Xuebao, 2018, 40(8): 138-151. doi: 10.3969/j.issn.0253-4193.2018.08.014

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Screening the diversity and activity of culturable bacteria isolated from mangrove rhizosphere soil at Shankou

doi: 10.3969/j.issn.0253-4193.2018.08.014

1.
Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, China
2.
Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, China;Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and their Modificationg, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China
3.
Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and their Modificationg, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China

Received Date: 2017-06-13
Rev Recd Date: 2018-02-13

Abstract

Abstract

Distribution of culturable bacteria in the Guangxi Shankou mangrove rhizosphere sediments was investigated in this study. 25 sediment samples collected from Guangxi mangrove rhizosphere sediments were used for bacterial diversity research by using the culture dependent method. 117 isolates were selected for further study. Phylogenetic analysis based on the partial 16S rRNA sequences clustered the 117 isolates into 9 classes, 20 orders, 27 families and 35 genera. 9 classes include Alphaproteobacteria(21.37%), Betaproteobacteria(1.71%), Gammaproteobacteria(12.82%), Deltabacteria(0.85%), Cytophagia(0.85%), Sphingobacteria (0.85%), Flavobacteria(2.56%), Actinobacteria(27.35%) and Bacilli(31.62%). The dominant genus was Bacillus, in which takes a great proportion of 28.20%. Additionally, 7 potential novel species from Alphaproteobacteria, Gammaproteobacteria, Flavobacteria and Actinobacteria were obtained. The results showed that bacteria producing amylase, protease, glucanase, cellulase and chitinase were abundant in the rhizosphere sediments of Shankou mangrove. Bacilli produced the most multiple enzymes. The preliminary study indicates that population diversity of culturable bacteria was abundant in the Guangxi Shankou mangrove rhizosphere sediments, and this worth further studies.
- mangrove,
- Bacilli,
- culturable bacteria,
- diversity,
- 16S rRNA sequences

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References(47)

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