Chemiluminescence detection of nitric oxide in seawater

FENG Wei-hua; LIU Chun-ying; YANG Gui-peng

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FENG Wei-hua, LIU Chun-ying, YANG Gui-peng. Chemiluminescence detection of nitric oxide in seawater[J]. Haiyang Xuebao, 2011, 33(6): 93-99.

Citation:

FENG Wei-hua, LIU Chun-ying, YANG Gui-peng. Chemiluminescence detection of nitric oxide in seawater[J]. Haiyang Xuebao, 2011, 33(6): 93-99.

FENG Wei-hua, LIU Chun-ying, YANG Gui-peng. Chemiluminescence detection of nitric oxide in seawater[J]. Haiyang Xuebao, 2011, 33(6): 93-99.

Citation:

FENG Wei-hua, LIU Chun-ying, YANG Gui-peng. Chemiluminescence detection of nitric oxide in seawater[J]. Haiyang Xuebao, 2011, 33(6): 93-99.

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Chemiluminescence detection of nitric oxide in seawater

Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China

Received Date: 2010-11-01
Rev Recd Date: 2011-05-08

Abstract

Abstract

A chemiluminescence method measuring the concentration of NO in seawater was established in this paper on the base of a reaction between the mixed solution of luminol-H₂O₂, the chemiluminescence probe, and NO, in which the chemiluminescence signal was emitted. The optimal results in the determination of NO concentration were under the conditions of a pH 9.2 buffer system with 1×10^-2 mol/dm³ carbonate, 8.4×10^-5 mol/dm³ luminol, 1×10^-2 mol/dm³ H₂O₂, and under 20 ℃ and 1×10^-3 mol/dm³ EDTA as a optimal masking agent. Under these optimal conditions, there was a significant linear relationship between the NO concentration and the peak area of chemiluminescence signal in the range of NO concentration from 1.4×10^-12 to 9.8×10^-10 mol/ dm³ (R=0.997, P <0.000 1). The detection limit for NO was 1.4×10^-12 mol/dm³ (S/N). In 7 times of parallel determination to 1.2×10^-11 mol/dm³NO aqueous solution, the relative standard deviation (RSD) was 13.6%. The NO concentration in the surface seawater of Jiaozhou Bay, China was successfully determined using this method and the results indicated that the concentrations of NO ranged from undetected to 7.1×10^-11 mol/ dm³ with an average of (1.6±1.8)×10^-11 mol/dm³.
- nitric oxide (NO),
- chemiluminescence,
- seawater,
- luminol-H₂O₂,
- Jiaozhou Bay

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References(20)

References

SCHREIBER F, POLERECKY L, DE BEER D.Nitric oxide microsensor for high spatial resolution measurements in biofilms and sediments[J]. Anal Chem, 2008, 80(4): 1152-1158.

DUNCAN C, DOUGALL H, JOHNSTON P, et al.Chemical generation of nitric oxide in the mouth from the enterosalivary circulation of dietary nitrate[J]. Nature Medicine, 1995, 1(6): 546-551.

张正斌,邢磊,吴真真,等. 海洋生态系中NO的发现和化学特征研究[J]. 中国科学:B辑, 2006, 49(5): 475-480.

刘春颖,张正斌,李培峰,等. 外源一氧化氮对亚心形扁藻的生长效应及光谱研究[J]. 环境科学, 2006, 27(6): 1062-1067.

张正斌,林彩,刘春颖,等. 一氧化氮对海洋浮游植物生长影响的规律及其化学特征研究[J]. 中国科学:B辑, 2004, 34(5): 393-401.

刘春颖,张正斌,陈小睿. 一氧化氮和铁对海洋微藻生长的交互影响[J]. 海洋学报, 2005, 24(5): 100-109.

KIECHLE F L, MALINSKI T. Nitric oxide: Biochemistry, pathophysiology and detection[J]. Am J Clin Pathol, 1993, 100: 567-575.

ARCHER S L, COWAN N. Measurement of endothelial cytosolic calcium concentration and nitric oxide production reveals discrete mechanisms of endothelium-dependent pulmonary vasodilatation[J]. Circulation, 1991, 68: 1569-1581.

ZAFIRIOU O C, MCFARLAND M. Determination of trace levels of nitric oxide in aqueous solution[J]. Anal Chem, 1980, 52: 1662-1667.

SAHA A, GOLDSTEIN S, CABELLI D, et al. Determination of optimal conditions for synthesis of peroxynitrite by mixing acidified hydrogen peroxide with nitrite[J]. Free Radical Biology and Medicine, 1998, 24(4): 653-659.

FEELISH M. The biochemical pathways of nitric oxide formation of nitrovasodilators: Appropriate choice of exogenous NO donors and aspects of preparation and handling of aqueous NO solutions[J]. Cardiovasc Pharmacol, 1991, 17: S25-S33

刘春颖,赵敏,任春艳,等. 荧光检测海水介质中的一氧化氮[J]. 分析化学, 2009, 37(10): 1463-1467.

KIKUCHI K, NAGANO T, HAYAKAWA H, et al. Detection of nitric oxide production from a perfused organ by a luminol-H₂O₂ system[J]. Anal Chem, 1993, 65(13): 1794-1799.

冯士筰,李凤岐,李少菁. 海洋科学导论[M]. 北京:高等教育出版社, 1999, 110-113.

ZAFIRIOU O C, MCFARLAND M, BROMUND R H. Nitric Oxide in Seawater[J]. Science New Series, 1980, 207(4431): 637-639.

OLASEHINDE E F, TAKEDA K, SAKUGAWA.H. Development of an Analytical Method for Nitric Oxide Radical Determination in Natural Waters[J]. Anal Chem, 2009, 81: 6843-6850.

ZAFIRIOU O C, MCFARLAND M. Nitric oxide from nitrite photolysis in the central equatorial pacific[J]. J Geophys Res, 1981, 86: 3173-3182.

RADI R, COSGROVE T, BECKMAN J, et al. Peroxynitrite-induced luminnol chemiluminescence[J]. Biochemical Journal, 1993, 290(1): 51-57.

任春艳. 天然海水介质中一氧化氮的电化学测定 . 青岛:中国海洋大学,2003.

邢磊. 海水介质中一氧化氮的电化学分析方法及一氧化氮对赤潮异弯藻影响研究 . 青岛:中国海洋大学,2004.

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