Isolation and characterization of oil-degrading marine micro-organisms

LIU Chen-li; SHAO Zong-ze

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LIU Chen-li, SHAO Zong-ze. Isolation and characterization of oil-degrading marine micro-organisms[J]. Haiyang Xuebao, 2005, 27(4): 114-120.

Citation:

LIU Chen-li, SHAO Zong-ze. Isolation and characterization of oil-degrading marine micro-organisms[J]. Haiyang Xuebao, 2005, 27(4): 114-120.

LIU Chen-li, SHAO Zong-ze. Isolation and characterization of oil-degrading marine micro-organisms[J]. Haiyang Xuebao, 2005, 27(4): 114-120.

Citation:

LIU Chen-li, SHAO Zong-ze. Isolation and characterization of oil-degrading marine micro-organisms[J]. Haiyang Xuebao, 2005, 27(4): 114-120.

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Isolation and characterization of oil-degrading marine micro-organisms

LIU Chen-li¹,
SHAO Zong-ze^2
,

1.
Life Science College, Xiamen University, Xiamen 361005, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

Received Date: 2004-07-01
Rev Recd Date: 2004-10-21

Abstract

Abstract

By enriching with diesel fUel as a sole carbon source,two microbial consortia were obtained from different marine environment samples:one,colleted from the Huanghe Dock of Shengli Oil Field,and the other,from the Oil storage Dock Xiamen.Nine bacteria and one fungus were screened out from the two samples.All of the isolates show considerable biodeg radability to diesel fuelex cept for M-5,while it shows degradability to several kinds of PAHs.Sequence analysis of 16S rDNA identifies that the 9 bacteria belong to Bacillus cereus,Pseudomonas stutzeri,Staphylococcus arlettae,Alcanivorax sp.,Alcaligenes denitrificans and alpha proteobacterium(iso late M-5),respectively.A mong them,each of isolates B-5 and M-5 belongs to a novel species.Two alkane monoxyg enase fragments ware amplified from isolate B-5 and Pseudomonas stutzeri M-2.And the fragmentret rieved from isolate B-5 seems to encode a partial novel alkane monoxyg enase.
- marine microorganisms,
- biodegradation,
- diesel fuel,
- alkane monooxygenase

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References(23)

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