cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in <i>Tegillarca granosa</i>

Ren Fuzhen; Yao Hanhan; Dong Yinghui; Zhou Xiaolong; Lin Zhihua

doi:10.3969/j.issn.0253-4193.2016.08.008

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Ren Fuzhen, Yao Hanhan, Dong Yinghui, Zhou Xiaolong, Lin Zhihua. cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa[J]. Haiyang Xuebao, 2016, 38(8): 73-82. doi: 10.3969/j.issn.0253-4193.2016.08.008

Citation:

Ren Fuzhen, Yao Hanhan, Dong Yinghui, Zhou Xiaolong, Lin Zhihua. cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa[J]. Haiyang Xuebao, 2016, 38(8): 73-82. doi: 10.3969/j.issn.0253-4193.2016.08.008

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cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa

doi: 10.3969/j.issn.0253-4193.2016.08.008

1.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
2.
Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
3.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received Date: 2015-10-23
Rev Recd Date: 2016-04-06

Abstract

Abstract

As an important member of HDACs family, HDAC1 can regulate gene expresstion and play a crucial role in cell differentiation and early embryonic development. Tg-HDAC1 cDNA was cloned by SMART RACE technique and then the bioinformatics, expression analysis, and intron amplification of Tg-HDAC1 were carried out in Tegillarca granosa. The full length of Tg-HDAC1 cDNA was 2 275 bp, containing a complete 1587 bp ORF encoding 528 amino acids. The homologous similarity between the blood clam and other species, such as Danio rerio, Gallus gallus, Mus musculus, was more than 80%, which indicate that HDAC1 is relatively conserved in the evolution. Thirteen introns of Tg-HDAC1 were amplified in ORF, which all of them follow the principle of GT-AG. The results of six tissue-specific expression by real time PCR showed that Tg-HDAC1 gene expressed in all tissues, and the expression of foot were significantly higher than other tissues(P<0.01), which suggest that the gene play an important role in the course of foot growth. The relative expression in different stages revealed that the expression of Tg-HDAC1 gradually increased with the process of the development, and showed the highest in trochophore stage (P<0.05).
- Tegillarca granosa,
- HDAC1,
- gene cloning,
- intron,
- expression analysis

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References(30)

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