Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters

Liu Ruijuan; Xiao Jie; Zhang Xuelei; Charatsee Aungtonya

doi:10.3969/j.issn.0253-4193.2016.06.006

Article Contents

Article Navigation > Haiyang Xuebao > 2016 > 38(6): 51-61

Liu Ruijuan, Xiao Jie, Zhang Xuelei, Charatsee Aungtonya. Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters[J]. Haiyang Xuebao, 2016, 38(6): 51-61. doi: 10.3969/j.issn.0253-4193.2016.06.006

Citation:

Liu Ruijuan, Xiao Jie, Zhang Xuelei, Charatsee Aungtonya. Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters[J]. Haiyang Xuebao, 2016, 38(6): 51-61. doi: 10.3969/j.issn.0253-4193.2016.06.006

Citation:

PDF( 2483 KB)

Genetic analysis of common venomous Cubozoa and Scyphozoa in Thailand waters

doi: 10.3969/j.issn.0253-4193.2016.06.006

1.
The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Key Laboratory of Science and Engineering for Marine Ecology and Environment, State Oceanic Administration, Qingdao 266061, China
2.
Phuket Marine Biological Center, Phuket 83000, Thailand

Received Date: 2015-11-16

Abstract

Abstract

The genetic diversity of common venomous Cubozoa and Scyphozoa jellyfish in the coastal waters of Thailand was investigated using the nuclear 18S and mitochondrial 16S rDNA genes. It was also compared about the capability of these two molecular markers to discriminate those common jellyfish species. There were 9 genetically distinctive species among the 32 samples collected from Thai waters, including 4 Cubozoa species, 4 Scyphozoans and 1 hydrozoan. However, it needs further information on the morphology and life cycle to determine the true taxonomic status of most species in this research. The K2P (Kimura 2-parameter) genetic distance was evaluated within and among the species based on the sequences of these two genes. The results showed much higher genetic divergence based on 16S gene (0－0.050 and 0.204－0.474 for intra-and inter-species genetic distances, respectively) compared with those using 18S gene (0－0.002 and 0.008－0.066 for intra-and inter-species genetic distances, respectively). At the same time, the A and T nucleotide composition of 16S gene was obviously higher than 18S gene. And significant variation of nucleotide composition was detected between two jellyfish classes using 16S gene, but not 18S gene. These results further indicated the relatively higher mutation rate of 16S gene, which could be applied for the genetic diversity studies among jellyfish species with close genetic relationship and the intra-species genetic population studies.
- Scyphozoa,
- Cubozoa,
- genetic diversity,
- ribosomal RNA gene (rDNA),
- Thailand

FullText(HTML)

References(33)

References

张芳, 孙松, 李超伦. 海洋水母类生态学研究进展[J]. 自然科学进展, 2009, 19(2): 121-130. Zhang Fang, Sun Song, Li Chaolun. Research advances in marine jellyfish ecology[J]. Process in Natural Science, 2009, 19(2): 121-130.

吴颖, 李惠玉, 李圣法, 等. 大型水母的研究现状及展望[J]. 海洋渔业, 2008, 30(1): 80-87. Wu Ying, Li Huiyu, Li Shengfa, et al. Review on the current situation of macro-jellyfish research and expectations[J]. Marine Fisheries, 2008, 30(1): 80-87.

于华华, 刘希光, 刘松, 等. 水母毒素的研究现状[J]. 海洋科学, 2003, 27(11): 27-29. Yu Huahua, Liu Xiguang, Liu Song, et al. The research status of jellyfish toxin[J]. Marine Sciences, 2003, 27(11): 27-29.

肖良, 张黎明. 水母毒素的研究进展[J]. 中国海洋药物, 2008, 26(6): 40-43. Xiao Liang, Zhang Liming. The research progress of jellyfish toxins[J]. Chinese Journal of Marine Drugs, 2008, 26(6): 40-43.

Suntrarachun S, Roselieb M, Wilde H, et al. A fatal jellyfish encounter in the Gulf of Siam[J]. Journal of Travel Medicine, 2001, 8(3): 150-151.

De Pender A M G, Winkel K D, Ligthelm R J. A probable case of Irukandji Syndrome in Thailand[J]. Journal of Travel Medicine, 2006, 13(4): 240-243.

Fenner P J, Lippmann J, Gershwin L A. Fatal and nonfatal severe jellyfish stings in Thai waters[J]. Journal of Travel Medicine, 2010, 17(2): 133-138.

Gershwin L A. Comments on Chiropsalmus(Cnidaria: Cubozoa: Chirodropida): a preliminary revision of the Chiropsalmidae, with descriptions of two new genera and two new species[J]. Zootaxa, 2006, 1231: 1-42.

Cornelius P F S. European Obelia(Cnidaria, Hydroida): systematics and identification[J]. Journal of Natural History, 1990, 24(3): 535-578.

王仁诚. 中国近海常见水母类和(虫戎)类的DNA条形码分析及系统发生研究[D]. 青岛: 中国科学院海洋研究所, 2010. Wang Rencheng. DNA barcoding and phylogenetic of Medusa and Hyperiid in Chinese Costal Region[D]. Qingdao: Institute of Oceanology, Chinese Academy of Sciences, 2010.

程方平, 王敏晓, 王彦涛, 等. 中国北方习见水母类的DNA条形码分析[J]. 海洋与湖沼, 2012, 43(3): 451-459. Cheng Fangping, Wang Minxiao, Wang Yantao, et al. DNA barcoding of common Medusozoa in Northern China based on mtCOI sequence[J]. Oceanologia et Limnologia Sinica, 2012, 43(3): 451-459.

Bayha K M. The molecular systematics and population genetics of four coastal ctenophores and scyphozoan jellyfish of the US Atlantic and Gulf of Mexico[D]. Newark: The University of Delaware, 2005.

Bayha K M, Dawson M N, Collins A G, et al. Evolutionary relationships among scyphozoan jellyfish families based on complete taxon sampling and phylogenetic analyses of 18S and 28S ribosomal DNA[J]. Integrative and Comparative Biology, 2010, 50(3): 436-455.

Tamura K, Stecher G, Peterson D, et al. MEGA6: molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 2013, 30(12): 2725-2729.

Tamura K, Dudley J, Nei M, et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Molecular Biology and Evolution, 2007, 24(8): 1596-1599.

Fuchs B, Wang Wei, Graspeuntner S, et al. Regulation of polyp-to-jellyfish transition in Aurelia aurita[J]. Current Biology, 2014, 24(3): 263-273.

王建艳, 甄毓, 王国善, 等. 基于mt-16S rDNA和mt-COI基因的海月水母分子生物学鉴定方法和检测技术[J]. 应用生态学报, 2013, 24(3): 847-852. Wang Jianyan, Zhen Yu, Wang Guoshan, et al. Molecular identification and detection of moon jellyfish (Aurelia sp.) based on partial sequencing of mitochondrial 16S rDNA and COI[J]. Chinese Journal of Applied Ecology, 2013, 24(3): 847-852.

Bentlage B, Cartwright P, Yanagihara A A, et al. Evolution of box jellyfish (Cnidaria: Cubozoa), a group of highly toxic invertebrates[J]. Proceedings of the Royal Society of London Series B, 2010, 277(1680): 493-501.

Collins A G, Winkelmann S, Hadrys H, et al. Phylogeny of Capitata and Corynidae (Cnidaria, Hydrozoa) in light of mitochondrial 16S rDNA data[J]. Zoologica Scripta, 2005, 34(1): 91-99.

Ki J S, Hwang D S, Shin K, et al. Recent moon jelly (Aurelia sp. 1) blooms in Korean coastal waters suggest global expansion: examples inferred from mitochondrial COI and nuclear ITS-5.8 S rDNA sequences[J]. ICES Journal of Marine Science, 2008, 65(3): 443-452.

Holst S, Laakmann S. Morphological and molecular discrimination of two closely related jellyfish species, Cyanea capillata and C. lamarckii (Cnidaria, Scyphozoa), from the northeast Atlantic[J]. Journal of Plankton Research, 2013, 36(1): 48-63.

Collins A G. Phylogeny of Medusozoa and the evolution of cnidarian life cycles[J]. Journal of Evolutionary Biology, 2002, 15(3): 418-432.

Evans N M, Lindner A, Raikova E V, et al. Phylogenetic placement of the enigmatic parasite, Polypodium hydriforme, within the Phylum Cnidaria[J]. BMC Evolutionary Biology, 2008, 8(1): 139.

张珰妮, 郑连明, 何劲儒, 等. 基于线粒体COI和16S片段序列的北部湾北部水螅水母DNA条形码分析[J]. 生物多样性, 2015, 23(1): 50-60. Zhang Dangni, Zheng Lianming, He Jinru, et al. DNA barcoding of Hydromedusae in northern Beibu Gulf for species identification[J]. Biodiversity Science, 2015, 23(1): 50-60.

Beard C B, Hamm D, Collins F H. The mitochondrial genome of the mosquito Anopheles gambiae: DNA sequence, genome organization, and comparisons with mitochondrial sequences of other insects[J]. Insect Molecular Biology, 1993, 2(2): 103-124.

Cartwright P, Evans N M, Dunn C W, et al. Phylogenetics of Hydroidolina (Hydrozoa: Cnidaria)[J]. Journal of the Marine Biological Association of the United Kingdom 2008, 88(8): 1663-1672.

Hwang U W, Kim W. General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics[J]. The Korean Journal of Parasitology, 1999, 37(4): 215-228.

张芳. 黄东海胶质浮游动物水母类研究[D]. 青岛: 中国科学院海洋研究所, 2008. Zhang Fang. Zooplanktivorous gelatinous taxa: medusas in the Yellow Sea and East China Sea[D]. Qingdao: Institute of Oceanology, Chinese Academy of Sciences, 2008.

Myers A G. Medusae of the world: the Scyphomedusae[M]. Washington DC: Carnegie Inst., 1910.

Ortman B D, Bucklin A, Pagès F, et al. DNA barcoding the medusozoa using mtCOI[J]. Deep-Sea Research Ⅱ: Topical Studies in Oceanography, 2010, 57(24/26): 2148-2156.

Lewis C, Bentlage B. Clarifying the identity of the Japanese Habu-kurage, Chironex yamaguchii, sp. nov. (Cnidaria: Cubozoa: Chirodropida)[J]. Zootaxa, 2009, 2030: 59-65.

Daly M, Brugler M R, Cartwright P, et al. The phylum Cnidaria: a review of phylogenetic patterns and diversity 300 years after Linnaeus[J]. Zootaxa, 2007, 1668: 127-182.

Dawson M N. Some implications of molecular phylogenetics for understanding biodiversity in jellyfishes, with emphasis on Scyphozoa[J]. Hydrobiologia, 2004, 530(1):249-260.

Relative Articles

Supplements(0)

Cited By

Proportional views