Gene rearrangement,molecular markers and phylogenetic analyses of echinoderms mitochondrial genomes

SHEN Xin; TIAN Mei; MENG Xueping; CHENG Hanliang; YAN Binlun

doi:10.3969/j.issn.0253-4193.2013.05.015

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SHEN Xin, TIAN Mei, MENG Xueping, CHENG Hanliang, YAN Binlun. Gene rearrangement,molecular markers and phylogenetic analyses of echinoderms mitochondrial genomes[J]. Haiyang Xuebao, 2013, 35(5): 137-146. doi: 10.3969/j.issn.0253-4193.2013.05.015

Citation:

SHEN Xin, TIAN Mei, MENG Xueping, CHENG Hanliang, YAN Binlun. Gene rearrangement,molecular markers and phylogenetic analyses of echinoderms mitochondrial genomes[J]. Haiyang Xuebao, 2013, 35(5): 137-146. doi: 10.3969/j.issn.0253-4193.2013.05.015

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Gene rearrangement,molecular markers and phylogenetic analyses of echinoderms mitochondrial genomes

doi: 10.3969/j.issn.0253-4193.2013.05.015

1.
College of Marine Science Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China;Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China
2.
College of Marine Science Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China

Received Date: 2012-12-05

Abstract

Abstract

Echinoderms are important group of invertebrates,which only found in marine habitats. Twenty-nine echinoderms mitochondrial genomes were fully analyzed in this article. The major coding gene arrangements of sea urchins and sea cucumbers are identical. The major coding gene arrangements within starfishes are also identical,and there is a long fragment inversion compared with these of sea urchins and sea cucumbers. The major coding gene arrangements of Phanogenia gracilis and Florometra serratissima(Crinoidea) are identical,compared with which there are a protein-coding genes (nad4L) translocation in Antedon mediterranea and Neogymnocrinus richeri mitochondrial genomes,respectively. The major coding gene arrangements of three families Amphiuridae,Ophiactidae and Ophiocomidae (Ophiuroidea: Ophiurida) are identical. However,there are three protein-coding genes (nad1,nad2 and cob) inversions in the mitochondrial genomes of Ophiura albida and Ophiura lutkeni,which belong to another family in the same order Ophiurida. Compared with five mitochondrial genomes from Ophiurida,there are major coding gene rearrangements in Astrospartus mediterraneus(Ophiuroidea: Euryalida) mitochondrial genome. The genetic variation analyses of main genes (13 protein coding genes) within 29 echinoderms mitochondrial genomes shown that nad5,nad4 and nad2 gene are ideal molecular markers. Tree topologies based on amino acid sequences of the protein-coding genes in 29 echinoderms mitochondrial genomes by two methods (neighbor-joining and maximum likelihood method) are identical. Phylogenetic trees based on mitochondrial genome data support that five classes (Ophiuroidea,Holothuroidea,Echinoidea,Asteroidea and Crinoidea) are monophyletic groups,and the relationship within them are (((Echinoidea+Asteroidea)+Holothuroidea)+Ophiuroidea)+Crinoidea. The class Crinoidea is the most ancient group in echinoderms,which located in the root of phylogenetic trees. Phylogenetic results support all families are monophyletic groups. Comprehensive analyses of phylogenetic trees based on the protein coding genes and major genes rearrangements support that Ophiurida is not monophyletic. The validity of the family Ophiurida is also worth further studies.
- mitochondrial genome,
- protein-coding gene,
- gene rearrangement,
- molecular marker,
- phylogeny,
- echinoderms

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References(24)

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