印度尼西亚峇淡岛海域鱾新本尼登虫（Neobenedenia girellae）形态学及28S rRNA分子鉴定
doi: 10.3969/j.issn.0253-4193.2020.06.014
Morphology and 28S rRNA identification of Neobenedenia girellae in cage aquaculture at the Batam Island, Indonesia.
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摘要: 本研究通过结合形态学与DNA分子标记等生物学手段，对印度尼西亚峇淡岛海域海水鱼类养殖中的本尼登虫(Isolate Batam, IB)进行形态学分析和种类鉴定。IB虫株在形态上与鱾新本尼登虫(Neobenedenia girellae)相似，属于新本尼登属。28S rRNA序列扩增得到序列为394 bp，与其他本尼登虫属比对相似性在85.86%～99.47%之间。进化树构建结果显示IB虫株与其他珻氏新本尼登虫(Neobenedenia melleni)和鱾新本尼登虫构成一簇，而本尼登虫自成一簇。综上所述，本研究结果支持鱾新本尼登虫与珻氏新本尼登虫为同种异名的分类学观点，并将IB虫株定种为鱾新本尼登虫。Abstract: In present study, the morphology and species identification of the Neobenedenia parasites (Isolate Batam/IB) in the cage aquaculture were studied via morphological and molecular marker methods at Batam Island, Indonesia. The isolate Batam is morphological similar with Neobenedenia girellae, and it is considered to belong to the Neobenedenia genera. 394 bp of the 28S rRNA was amplified, and its similarity with other genera was between 85.86%−99.47%. The phylogenetic tree shows that IB, Neobenedenia melleni and Neobenedenia girellae are in a same cluster, and other Benedeniinae species form their own cluster. To sum up, IB is identified as the specie of Neobenedenia girellae and we support the theory that N. melleni and N. girellae are synonyms.
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Key words:
- Neobenedenia /
- morphological identification /
- 28S rRNA /
- Indonesia
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图 1 鱾新本尼登虫峇淡岛虫株形态图
Fig. 1 Morphological structure of Neobenedenia girellae isolate Batam
图 2 鱾新本尼登虫光镜观察
A. 虫体前端；B. 虫体中部；C. 虫体后端；Eye，眼点；Pha，咽；Sv，储精囊；Vr，卵黄储囊；Ov，卵巢；Ic，卵巢内腔；Te，睾丸；as，附甲片；ah，前钩；ph，后钩；m，边缘膜
Fig. 2 Morphology of Neobenedenia girellae isolate Batam
A. Front part of the parasite; B. Middle part of the parasite; C. Back part of the parasite; Eye，eyespot; Pha，pharynx；Sv，seminal vesicle；Vr，vitelline sac；Ov，ovary；Ic，insemination sac；Te，testis；as，accessory sclerite；ah，anterior hamulus；ph，posterior hamulus；m，marginal membrane
图 3 鱾新本尼登虫28S rRNA序列同源比对
Fig. 3 Homology comparison of 28S rRNA of Neobenedenia girellae isolate Batam
图 4 鱾新本尼登虫系统进化树
红色字体显示IB虫株Neobenedenia girellae isolate Batam
Fig. 4 Phylogenetic tree analysis of Neobenedenia girellae isolate Batam
Neobenedenia girellae isolate Batam is indicated with red font
表 1 N. girellae isolate Batam与其他新本尼登虫形态比较
Tab. 1 Comparison of morphological characters of N. girellae isolate Batam with other similar species
编号 名称 体长/μm 体宽/μm 前吸器 后吸器 咽 长/μm 宽/μm 长/μm 宽/μm 长/μm 宽/μm 1 N. girellae isolate Batam MN209942 3 275.33±
516.121 922.41±
189.27364.37±
70.75323.61±
51.621 049.25±
144.621 014.10±
164.83390.37±
109.24520.94±
101.412 N. girellae 4 191.70 2 083.3 338.7 369.3 851.3 897 329.3 310 3 N. melleni 3 191.80 1 792.4 243 289.6 919.2 911.2 389.2 285.4 4 N. congeri 2 132±218.6 834±136.7 22223.87 230±18.87 474±52.25 544±60.25 186±24.08 260±23.45 5 N. pargueraensis 2 910.00 1 525 240 265 690 770 335 259 6 N. adenae 2 459.70 1 579.8 196.2 176.7 721.8 688.5 300.3 205.8 7 N. isabellae 4 857.20 2 350.1 310.4 363.3 1 121.4 1 189.9 405.6 281 编号 名称 边缘膜
/μm附甲片
/μm前钩长
/μm后钩长
/μm边缘小钩
/μm卵巢
/μm睾丸
/μm长/μm 宽/μm 长/μm 宽/μm 1 N. girellae isolate Batam MN209942 44.82±
7.28201.12±
21.98301.05±
46.7890.49±
30.53− 262.47±
21.83296.28±
67.38398.31±
45.77308.23±
47.572 N. girellae − 195 287 109.7 9.3 − − − − 3 N. melleni − 176.2 277.8 120.2 9 − − − − 4 N. congeri − 128±1.37 121±12.07 87±7.98 10.75±1.12 140±24.5 180±44.16 198±32.7 304±48.3 5 N. pargueraensis − 155 150 95.5 7 − − − − 6 N. adenae − 102.8 202.3 59 34.7 − − − − 7 N. isabellae − 198 362.8 82.5 10.9 − − − − 注：−代表无数据。 
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