急性低氧胁迫对军曹鱼大规格幼鱼血液生化指标的影响

黄建盛; 陆枝; 陈刚; 张健东; 郭志雄; 洪越群

doi:10.3969/j.issn.0253-4193.2019.06.007

急性低氧胁迫对军曹鱼大规格幼鱼血液生化指标的影响

doi: 10.3969/j.issn.0253-4193.2019.06.007

1.
广东海洋大学水产学院, 广东湛江 524088
2.
广东越群海洋生物研究开发有限公司, 广东揭阳 515200

基金项目: 现代农业产业技术体系专项资金资助（CARS-47），广东海洋大学大学生创新创业训练计划项目（CXXL2018131）。

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出版历程
- 收稿日期: 2018-09-02
- 修回日期: 2018-10-18

Acute hypoxia stress on blood biochemical indexes of large-sized juvenile cobia (Rachycentron canadum)

1.
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China
2.
Guangdong Yuequn Marine Biological Research and Development Co., Ltd., Jieyang 515200, China

摘要

摘要: 为了研究军曹鱼在急性低氧应激时体内物质代谢和组织器官机能状态变化，分别测定低氧胁迫前后血液生化指标的含量。结果表明：（1）急性低氧胁迫后，军曹鱼血清电解质含量呈不同程度升高，其中钠、氯和钙离子浓度与胁迫前差异极显著（P<0.01）；钾离子浓度与胁迫前无显著性差异（P>0.05）。（2）急性低氧胁迫后，军曹鱼血清肝功能指标变化情况各异。其中，白蛋白含量与胁迫前略有降低，谷丙转氨酶和谷草转氨酶活性与胁迫前略有上升，但差异均不具有统计学意义（P>0.05），总蛋白含量显著低于胁迫前（P<0.05），白蛋白和球蛋白比值则显著高于胁迫前（P<0.05），碱性磷酸酶极显著低于胁迫前（P<0.01）。（3）急性低氧胁迫后，军曹鱼血清葡萄糖和肌酐浓度均呈升高的变化，且与胁迫前差异极显著（P<0.01）。（4）急性低氧胁迫后，血清肌酸激酶和乳酸脱氢酶活性均呈升高变化，肌酸激酶活性与胁迫前差异显著（P<0.05），乳酸脱氢酶活性与胁迫前差异极显著（P<0.01）。（5）低氧胁迫后，军曹鱼血脂指标变化情况各异，其中总胆固醇和高密度脂蛋白浓度呈极显著降低的变化（P<0.01），低密度脂蛋白浓度呈显著升高的变化（P<0.05），甘油三酯浓度略有升高，但不具有统计学意义（P>0.05）。研究表明，急性低氧胁迫后，军曹鱼大部分血液生化指标发生显著性变化，预示着肝脏、肾脏及心脏组织造成损伤，使其机能出现紊乱。
- 军曹鱼 /
- 急性低氧 /
- 血液 /
- 生化指标
Abstract: The blood biochemical indexes were measured to evaluate the effects of acute hypoxia stress on body metabolism and tissue function of cobia (Rachycentron canadum). Results of this study are:(1) electrolyte content in serum increases in response to the acute hypoxia stress; serum ion concentrations of sodium, chloride and calcium are significantly changed(P<0.01), however, no significant difference on the level of potassium concentration was detected. (2) As for the indexes of liver function in cobia under acute hypoxic stress, the albumin content slightly decreases, the alanine aminotransferase and aspartate aminotransferase activities slightly increase; the total protein content is lower than those of the fish with no treatment (P<0.05). The albumin/globulin ratio is significantly higher than that before stress (P<0.05), while alkaline phosphatase is significantly different from that before stress (P<0.01). (3) After acute hypoxia stress, the concentrations of serum glucose and creatinine in cobia significantly increase (P<0.01). (4) Serum creatine kinase and lactate dehydrogenase activities increase under acute hypoxia stress. Creatine kinase activity is significantly different from that before stress (P<0.05), while lactate dehydrogenase activity is significantly different from that before stress (P<0.01). (5) After hypoxic stress, the changes of blood lipid indexes are different. Among them, the total cholesterol and high density lipoprotein concentrations show a very significant decrease (P<0.01). The concentration of low density lipoprotein increases significantly (P<0.05), and the concentration of triglyceride increases slightly, but it is not statistically significant (P>0.05). These results indicate that most of the blood biochemical indicators of cobia show significant changes after acute hypoxic stress, indicating that the liver, kidney and heart tissue may have been damaged.
- Rachycentron canadum /
- acute hypoxia stress /
- blood /
- biochemical indexes

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参考文献(24)

常志成, 温海深, 张美昭, 等. 溶解氧水平对花鲈幼鱼氧化应激与能量利用的影响及生理机制[J]. 中国海洋大学学报:自然科学版, 2018, 48(7):20-28. Chang Zhicheng, Wen Haishen, Zhang Meizhao, et al. Effects of dissolved oxygen levels on oxidative stress response and energy utilization of juvenile Chinese sea bass (Lateolabrax maculatus) and associate physiological mechanisms[J]. Periodical of Ocean University of China, 2018, 48(7):20-28.

Vaquer-Sunyer R, Duarte C M. Thresholds of hypoxia for marine biodiversity[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(40):15452-15457.

Diaz R J. Overview of hypoxia around the world[J]. Journal of Environmental Quality, 2001, 30(2):275-281.

区又君, 范春燕, 李加儿, 等. 急性低氧胁迫对卵形鲳鲹选育群体血液生化指标的影响[J]. 海洋学报, 2014, 36(4):126-131. Ou Youjun, Fan Chunyan, Li Jiaer, et al. Acute hypoxia stress on blood biochemical indexes in selective group of Trachinotus ovatus[J]. Haiyang Xuebao, 2014, 36(4):126-131.

王晓雯, 朱华, 胡红霞, 等. 低氧胁迫对西伯利亚鲟幼鱼生理状态的影响[J]. 水产科学, 2016, 35(5):459-465. Wang Xiaowen, Zhu Hua, Hu Hongxia, et al. Effects of hypoxia on physiological status of siberian sturgeon Acipenser baeri juveniles[J]. Fisheries Science, 2016, 35(5):459-465.

张曦, 付世建, 彭姜岚, 等. 急性低氧对鲫鱼幼鱼血液基础指标的影响[J]. 重庆师范大学学报:自然科学版, 2011, 28(4):19-22. Zhang Xi, Fu Shijian, Peng Jianglan, et al. The effect of acute hypoxia on blood parameters of juvenile crucian carp[J]. Journal of Chongqing Normal University:Natural Science, 2011, 28(4):19-22.

王慧娟. 低氧对团头鲂生理生化指标及低氧应答基因表达的影响[D]. 武汉:华中农业大学, 2015. Wang Huijuan. Hypoxia effect on changes of biochemical and physiological parameters and the expression of hypoxia response genes in Megalobrama amblycephala[D]. Wuhan:Huazhong Agricultural University, 2015.

杨健, 陈刚, 黄建盛, 等. 温度和盐度对军曹鱼幼鱼生长与抗氧化酶活性的影响[J]. 广东海洋大学学报, 2007, 27(4):25-29. Yang Jian, Chen Gang, Huang Jiansheng, et al. Effects of temperature and salinity on the growth and activities of antioxidant enzymes of cobia (Rachycentron canadum) juveniles[J]. Journal of Guangdong Ocean University, 2007, 27(4):25-29.

汤保贵, 陈刚, 张健东, 等. 饵料系列对军曹鱼仔鱼生长、消化酶活力和体成分的影响[J]. 水生生物学报, 2007, 31(4):479-484. Tang Baogui, Chen Gang, Zhang Jiandong, et al. Effect of diet series on growth, activity of digestive enzymes and body composition of larval cobia, Rachycentron canadum[J]. Acta Hydrobiologica Sinica, 2007, 31(4):479-484.

陈刚, 周晖, 张健东, 等. 军曹鱼血液指标及血细胞发生的观察[J]. 水生生物学报, 2005, 29(5):564-570. Chen Gang, Zhou Hui, Zhang Jiandong, et al. Hematological study and observation on development of blood cells in cobia, Rachycentron canadum[J]. Acta Hydrobiologica Sinica, 2005, 29(5):564-570.

陈刚, 张健东, 吴灶和. 军曹鱼骨骼系统的研究[J]. 湛江海洋大学学报, 2004, 24(6):6-10. Chen Gang, Zhang Jiandong, Wu Zaohe. Morphological observation on the skeleton of cobia, Rachycentron canadum (Linnaeus)[J]. Journal of Zhanjiang Ocean University, 2004, 24(6):6-10.

初庆柱, 张健东, 陈刚, 等. 军曹鱼消化系统胚后发育的组织学研究[J]. 水生态学杂志, 2010, 3(4):86-91. Chu Qingzhu, Zhang Jiandong, Chen Gang, et al. Histological studies on postembryonic development of digestive system in Rachycentron canadum[J]. Journal of Hydroecology, 2010, 3(4):86-91.

王忠良, 黄亿彬, 陈刚, 等. 饥饿与补偿生长对军曹鱼幼鱼能量收支的影响[J]. 吉首大学学报:自然科学版, 2012, 33(6):102-106. Wang Zhongliang, Huang Yibin, Chen Gang, et al. Effects of starvation and compensatory growth on energy budget of juvenile cobia Rachycentron canadum[J]. Journal of Jishou University:Natural Science Edition, 2012, 33(6):102-106.

van Nguyen M, Phan L M T. Influences of bleeding conditions on the quality and lipid degradation of cobia (Rachycentron canadum) fillets during frozen storage[J]. Turkish Journal of Fisheries and Aquatic Sciences, 2018, 18(2):289-300.

Baldi S C V, Parisi G, Bonelli A, et al. Effects of different stunning/slaughter methods on frozen fillets quality of cobia (Rachycentron canadum)[J]. Aquaculture, 2018, 486:107-113.

Tran H B, Lee Y H, Guo J J, et al. De novo transcriptome analysis of immune response on cobia (Rachycentron canadum) infected with Photobacterium damselae subsp. piscicida revealed inhibition of complement components and involvement of MyD88-independent pathway[J]. Fish & Shellfish Immunology, 2018, 77:120-130.

何远法, 郭勇, 迟淑艳, 等. 低鱼粉饲料中补充蛋氨酸对军曹鱼生长性能、体成分及肌肉氨基酸组成的影响[J]. 动物营养学报, 2018, 30(2):624-634. He Yuanfa, Guo Yong, Chi Shuyan, et al. Effects of methionine supplementation in low fish meal diet on growth performance, body composition and muscle amino acid composition of cobia (Rachycentron canadum)[J]. Chinese Journal of Animal Nutrition, 2018, 30(2):624-634.

王震, 徐玮, 麦康森, 等. 饲料缬氨酸水平对军曹鱼鱼体脂肪含量、血浆生化指标和肝脏脂肪代谢基因表达的影响[J]. 水生生物学报, 2016, 40(4):744-751. Wang Zhen, Xu Wei, Mai Kangsen, et al. The effects of valine level on plasma biochemical indexes, lipid content and gene expression involved in lipid metabolism in cobia (Rachycentron canadum)[J]. Acta Hydrobiologica Sinica, 2016, 40(4):744-751.

Sakthivel M, Jayakumar R, Nazar K A, et al. Effect of temperature on yolk sac utilisation and growth of newly hatched larvae of cobia Rachycentron canadum (Linnaeus, 1766)[J]. Indian Journal of Fisheries, 2016, 63(3):135-139.

魏华, 吴垠. 鱼类生理学[M]. 北京:中国农业出版社, 2011. Wei Hua, Wu Yin. Fish Physiology[M]. Beijing:China Agriculture Press, 2011.

肖武汉. 低氧信号传导途径与鱼类低氧适应[J]. 中国科学:生命科学, 2014, 44(12):1227-1235. Xiao Wuhan. The hypoxia signaling pathway and hypoxic adaptation in fishes[J]. Science China Life Sciences, 2015, 58(2):148-155.

Tzaneva V, Vadeboncoeur C, Ting J, et al. Effects of hypoxia-induced gill remodelling on the innervation and distribution of ionocytes in the gill of goldfish, Carassius auratus[J]. The Journal of Comparative Neurology, 2014, 522(1):118-130.

温海深, 吕里康, 李兰敏, 等. 急性高温胁迫对雄性许氏平鲉血液生理生化及相关基因表达的影响[J]. 中国海洋大学学报, 2016, 46(11):44-51. Wen Haishen, Lv Likang, Li Lanmin, et al. Effect of temperature on physiological and biochemical parameters and gene expression of male Sebastes schlegelii[J]. Periodical of Ocean University of China, 2016, 46(11):44-51.

苏汉文, 李栋, 李艳. 急性心肌梗死血清标志物临床应用的比较[J]. 四川医学, 2006, 27(11):1125-1127. Su Hanwen, Li Dong, Li Yan. Comparison of serum marker for the clinical value of acute myocardial infarction[J]. Sichuan Medical Journal, 2006, 27(11):1125-1127.

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