碳氮稳定同位素比值在潮间带大型底栖动物组织间差异性研究

刘博; 陈琳琳; 李宝泉; 侯西勇; 冯光海; 李晓炜; 李秉钧; 王玉珏

doi:10.3969/j.issn.0253-4193.2019.04.007

碳氮稳定同位素比值在潮间带大型底栖动物组织间差异性研究

doi: 10.3969/j.issn.0253-4193.2019.04.007

1.
烟台大学海洋学院, 山东烟台 264005;中国科学院烟台海岸带研究所, 山东烟台 264003
2.
中国科学院烟台海岸带研究所, 山东烟台 264003
3.
黄河三角洲国家级自然保护区大汶流管理站, 山东东营 257500
4.
烟台大学海洋学院, 山东烟台 264005

基金项目: 美丽中国生态文明建设科技工程专项（XDA23050202，XDA23050304）；国家自然科学基金项目（41776126）；中国科学院烟台海岸带研究所自主部署项目（YICY755021012）；中国科学院前沿科学重点研究项目（QYZDB-SSW-DQC041）。

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出版历程
- 收稿日期: 2018-04-16
- 修回日期: 2018-12-05

Carbon and nitrogen stable isotopes variations in different tissues of macrobenthos in the intertidal zone

1.
School of Ocean, Yantai University, Yantai 264005, China;Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
2.
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
3.
Dawenliu Station of the River Delta National Nature Reserve, Dongying 257500, China
4.
School of Ocean, Yantai University, Yantai 264005, China

摘要

摘要: 近年来，稳定同位素技术已被广泛应用于食物网研究中。然而，生物样品取样部位对食物网构建的影响的研究相对少见。大型底栖动物作为潮间带食物网的重要组成部分，在食物网研究中有必要对其采样部位进行统一，以增加研究结果的准确性及可比较性。本研究选取黄河三角洲和烟台潮间带10种代表性的大型底栖动物，比较δ¹³C和δ¹⁵N在不同采样部位中的差异。结果显示，对于大多数底栖动物而言，采样部位将直接影响到δ¹³C和δ¹⁵N的测定结果。当底栖动物作为摄食者，需分析其食性和营养级时，应统一采用肌肉组织，如贝类的闭壳肌或足部肌肉，蟹类的鳌足肌肉，鱼类的背部肌肉；当底栖动物作为被摄食者，需分析次级消费者的食性和营养级时，除多毛类采用去除消化道内容物的体壁外，其他种类应选取整体（难以被消化的组织除外），如贝类的软体部，蟹类肌肉与鳃等的混合组织。
- 潮间带 /
- 大型底栖动物 /
- 碳氮稳定同位素 /
- 食源和营养级分析 /
- 采样部位选取
Abstract: The technique of stable isotope analysis has been widely applied for the field of food web studies in recent years. However, few researches concerned about the impacts of different tissues used for analysis on the food web building. With the purpose of accuracy and comparability in the food web studies, the sampling tissues should be standardized. In this study, 10 macrobenthic species from the intertidal zone of the Yellow River Delta and Yantai were chosen as the target object to test the isotopic characteristic variations of δ¹³C and δ¹⁵N at different body parts (whole bodies, muscles and gills). Our results show that δ¹³C and δ¹⁵N values vary in different sampling tissues for most species, which indicates the different tissues used for analysis impact the food web building. To achieve a more accuracy and comparability result, suitable tissue should be primarily chosen for their food sources and trophic levels analysis. Namely, when the organism as predator, their muscles should first be chosen, for examples, adductor and foot muscles of mollusks, leg muscles of crabs and back muscles of fishes; when the organism as prey, most of their tissues, except for indigestible part (for polychaetes, the digestive tract contents should be removed) should be chosen for analysis on food sources and trophic levels of the secondary consumer, for example, whole soft parts of mollusks, the mixture of muscles and gills of crabs.
- intertidal zone /
- macrobenthos /
- carbon and nitrogen stable isotopes /
- food sources and trophic levels study, sampling parts /

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