Feeding habits of Sphaeroma retrolaeve based on stable isotope analysis
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摘要: 为了探究光背团水虱的食性特征,本研究利用碳、氮稳定同位素技术于2015年冬季和2016年夏季对广西北海廉州湾红树林中光背团水虱及其食物来源的碳、氮稳定同位素比值(δ13C值和δ15N值)进行分析。结果显示,冬季和夏季光背团水虱的δ13C值大小范围为-22.85‰~-21.87‰,平均值为(-22.46±0.35)‰;δ15N值大小范围为11.02‰~12.85‰,平均值为(11.88±0.56)‰;光背团水虱的δ13C值、δ15N值变化范围较小,表明其食物来源较为简单。单因素方差分析结果显示,冬季与夏季光背团水虱的平均δ13C值差异不显著(P>0.05),而夏季的δ15N值普遍高于冬季δ15N值,差异显著(P<0.05);不同生长阶段的光背团水虱δ13C值、δ15N值会随着体长的增长而增大,差异显著(P<0.05),表明光背团水虱在生长的过程中可能发生了食性转变。光背团水虱的δ13C值与浮游生物的δ13C值相近,而与红树植物δ13C值差距较远,说明光背团水虱主要以浮游生物为食物来源。基于R语言稳定同位素混合模型(SIAR)计算结果显示,冬季和夏季各粒径级别浮游生物对不同生长阶段的光背团水虱的贡献率趋势基本一致,表现为1.2~25 μm粒级的浮游生物对光背团水虱平均贡献率最高,其次为25~50 μm粒级,粒径大于100 μm的浮游生物对体长小于5.5 mm的光背团水虱贡献率较低,对体长大于5.5 mm的光背团水虱的贡献率随着体长增大而相应增大,说明不同生长阶段的光背团水虱食性有差异。对光背团水虱食性分析的结果可为深入研究团水虱爆发的原因及危害红树林的作用机理提供基础资料。Abstract: In order to explore the feeding characteristics of Sphaeroma retrolaeve, we analyzed the carbon and nitrogen isotopic ratios (δ13C values, δ15N values) of S. retrolaeve and its food sources which were collected from the mangrove of Lianzhou Bay, Beihai, Guangxi, in the winter of 2015 and summer of 2016, based on the stable carbon and nitrogen isotope analysis. The results showed that the average δ13C and δ15N values of S. retrolaeve were (-22.46±0.35) ‰ and (11.88±0.56)‰, in a range of -22.85‰ to -21.87‰ and 11.02‰ to 12.85‰, respectively. The range of δ13C value and the δ15N value of S. retrolaeve was small, which indicated that the food sources of S. retrolaeve were relatively simple. The results of one-way ANOVA showed that the δ13C values of S. retrolaeve were insignificantly different between winter and summer (P>0.05), while the δ15N values in summer were generally higher than those in winter, and the difference was significant (P<0.05); δ13C and δ15N values of S. retrolaeve of different growth stages significantly correlated with body size (P<0.05), which indicated that its feeding habits shift during its growth process. The δ13C values of S. retrolaeve were similar to the δ13C values of the plankton, which were far different from the δ13C values of the mangrove plants, indicating that the main food sources of S. retrolaeve was plankton. The results of SIAR mixing model analysis showed that contribution rates of plankton to different growth stages of S.retrolaeve were basically the same in winter and summer. The plankton with the fraction of 1.2-25 μm had the highest contribution to S. retrolaeve, and followed by the fraction of 25-50 μm, the fraction more than 100 μm had a lower contribution to S. retrolaeve with body length less than 5.5 mm, while the proportion of contribution would increase with increasing body length, which showed that there were something differences in the feeding habits for S. retrolaeve at different growth stages. The results of the feeding habits of S. retrolaeve could provide basic information for further studying the causes of Sphaeroma outbreak and the mechanism of damage to mangroves.
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Key words:
- Sphaeroma retrolaeve /
- food habits /
- plankton /
- stable isotope
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于海燕, 李新正. 中国近海团水虱科种类记述[J]. 海洋科学集刊, 2003, 45:239-259. Yu Haiyan, Li Xinzheng. Study on the species of Sphaeromatidae from Chinese waters[J]. Studia Marina Sinica, 2003, 45:239-259. 刘瑞玉. 中国海洋生物名录[M]. 北京:科学出版社, 2008:690-698. Liu Ruiyu. Checklist of Marine Biota of China Seas[M]. Beijing:Science Press, 2008:690-698. 黄戚民, 周时强, 李复雪. 福建红树林上钻孔动物的生态[J]. 台湾海峡, 1996, 15(3):305-309. Huang Qimin, Zhou Shiqiang, Li Fuxue. Ecological studies on mangrove boring animals Fujian[J]. Journal of Oceanography in Taiwan Strait, 1996, 15(3):305-309. 范航清, 刘文爱, 钟才荣, 等. 中国红树林蛀木团水虱危害分析研究[J]. 广西科学, 2014(2):140-146, 152. Fang Hangqing, Liu Wen'ai, Zhong Cairong, et al. Analytic study on the damages of wood-boring isopod, sphaeroma, to China mangroves[J]. Guangxi Sciences, 2014(2):140-146, 152. Venkatakrishnan R, Nair N B. Observations on the sex characters of the timber-boring sphaeromids (Isopoda) of the Indian waters[J]. Hydrobiologia, 1973, 42(4):413-427. Thiel M. Reproductive biology of a wood-boring isopod, Sphaeroma terebrans, with extended parental care[J]. Marine Biology, 1999, 135(2):321-333. Baratti M, Filippelli M, Messana G. Complex genetic patterns in the mangrove wood-borer Sphaeroma terebrans Bate, 1866(Isopoda, Crustacea, Sphaeromatidae) generated by shoreline topography and rafting dispersal[J]. Journal of Experimental Marine Biology and Ecology, 2011, 398(1/7):73-82. 邱勇, 李俊, 黄勃, 等. 影响东寨港红树林中光背团水虱分布的生态因子研究[J]. 海洋科学, 2013, 37(4):21-25. Qiu Yong, Li Jun, Huang Bo, et al. Studies on ecological factors affecting the distribution of Sphraeroma retrolaevis in the mangrove forest in Dongzhai-gang[J]. Marine Sciences, 2013, 37(4):21-25. Li X F, Han C, Zhong C R, et al. Identification of Sphaeroma terebrans via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene[J]. Zoological Research, 2016, 37(5):307-312. Rotramel G. Filter-feeding by the marine boring isopod, Sphaeroma quoyanum H. Milne Edwards, 1840(Isopoda, Sphaeromatidae)[J]. Crustaceana, 1975, 28(1):7-10. Si A, Bellwood O, Alexander C G. Evidence for filter-feeding by the wood-boring isopod, Sphaeroma terebrans (Crustacea:Peracarida)[J]. Journal of Zoology, 2002, 256(4):463-471. Benson L K, Rice S A, Johnson B R. Evidence of cellulose digestion in the wood boring isopod Sphaeroma terebrans[J]. Florida Scientist, 1999:128-144. 邱勇. 光背团水虱消化酶及其种群生态学研究[D]. 海口:海南大学, 2013. Qiu Yong. Study on digestive enzyme and population ecology of Splraeroma retrolaevis[D]. Haikou:Hainan University, 2013. 胡亚强, 丁敬敬, 黄勃, 等. 团水虱不同生长阶段肠道微生物多样性分析[J]. 基因组学与应用生物学, 2016, 35(2):406-413. Hu Yaqiang, Ding Jingjing, Huang Bo, et al. Intestinal microbial diversity analysis of sphaeroma at different growth stages[J]. Genomics and Applied Biology, 2016, 35(2):406-413. Bouillon S, Koedam N, Raman A, et al. Primary producers sustaining macro-invertebrate communities in intertidal mangrove forests[J]. Oecologia, 2002, 130(3):441-448. Balasse M, Tresset A, Ambrose S H. Stable isotope evidence (δ13C, δ18O) for winter feeding on seaweed by Neolithic sheep of Scotland[J]. Journal of Zoology, 2006, 270(1):170-176. Fry B. Stable Isotope Ecology[M]. New York:Springer-Verlag, 2006. Parnell A C, Inger R, Bearhop S, et al. Source partitioning using stable isotopes:coping with too much variation[J]. PLoS One, 2010, 5(3):e9672. Post D M. Using stable isotopes to estimate trophic position:models, methods, and assumptions[J]. Ecology, 2002, 83(3):703-718. Tue N T, Hamaoka H, Sogabe A, et al. Food sources of macro-invertebrates in an important mangrove ecosystem of Vietnam determined by dual stable isotope signatures[J]. Journal of Sea Research, 2012, 72:14-21. 王瑁, 高雪芹, 丁弈朋, 等. 海南清澜港不同栖息位置红树林软体动物的食物来源[J]. 湿地科学, 2015, 13(2):171-176. Wang Mao, Gao Xueqin, Ding Yipeng, et al. Identification of the food sources of mangrove molluscs from different microhabitats at Qinglangang, Hainan[J]. Wetland Science, 2015, 13(2):171-176. 孙明, 刘修泽, 李轶平, 等. 应用氮稳定同位素技术研究辽东湾海域主要渔业生物的营养级[J]. 中国水产科学, 2013, 20(1):190-198. Sun Ming, Liu Xiuze, Li Yiping, et al. Trophic level analysis of key species in Liaodong Bay using stable nitrogen isotopes[J]. Journal of Fishery Sciences of China, 2013, 20(1):190-198. 蒋日进, 章守宇, 毕远新, 等. 枸杞岛海藻场小型无脊椎动物的食物来源[J]. 水产学报, 2015, 39(10):1487-1498. Jiang Rijin, Zhang Shouyu, Bi Yuanxin, et al. Food sources of small invertebrates in the macroalgal bed of Gouqi Island[J]. Journal of Fisheries of China, 2015, 39(10):1487-1498. 宁加佳, 刘辉, 古滨河, 等. 溪流河水库颗粒有机物及浮游动物碳、氮稳定同位素特征[J]. 生态学报, 2012, 32(5):1502-1509. Ning Jiajia, Liu Hui, Gu Binhe, et al. Carbon and nitrogen stable isotope characteristics of particulate organic matter and zooplankton in Liuxihe Reservoir[J]. Acta Ecologica Sinica, 2012, 32(5):1502-1509. 宁加佳, 杜飞雁, 王雪辉, 等. 基于稳定同位素的口虾蛄食性分析[J]. 水产学报, 2016, 40(6):903-910. Ning Jiajia, Du Feiyan, Wang Xuehui, et al. Feeding habits of mantis shrimp based on stable isotope analysis[J]. Journal of fisheries of China, 2016, 40(6):903-910. 薛彬, 蒋日进, 王凯, 等. 应用稳定同位素技术分析枸杞岛近岸海域褐菖鲉的食性[J]. 2017, 41(9):1415-1423. Xue Bin, Jiang Rijin, Wang Kai, et al. Feeding habits of Sebastiscus marmoratus in the Gouqi Island based on stable istope analysis[J]. Journal of fisheries of China, 2017, 41(9):1415-1423. Bouillon S, Mohan P C, Sreenivas N, et al. Sources of suspended organic matter and selective feeding by zooplankton in an estuarine mangrove ecosystem as traced by stable isotopes[J]. Marine Ecology Progress Series, 2000, 208:79-92. 蔡德陵, 孟凡, 韩贻兵, 等. 13C/12C比值作为海洋生态系统食物网示踪剂的研究——崂山湾水体生物食物网的营养关系[J]. 海洋与湖沼, 1999, 30(6):671-678. Cai Deling, Meng Fan, Han Yibing, et al. Studies on 13C/12C ratios as a tracer for food web in a marine ecosystem-The trophic relations in pelagic food webs in Laoshan Bay[J]. Oceanologia et Limnologia Sinica, 1999, 30(6):671-678. 冯建祥. 互花米草入侵和利用本土红树植物防控情境下红树林湿地食物网关系研究[D]. 厦门:厦门大学, 2013. Feng Jianxiang. Effects of the invasion and ecological control of Spartina alterniflora on the foodweb of mangrove wetlands[D]. Xiamen:Xiamen University, 2013. Grey J, Jones R I, Sleep D. Seasonal changes in the importance of the source of organic matter to the diet of zooplankton in Loch Ness, as indicated by stable isotope analysis[J]. Limnology and Oceanography, 2001, 46(3):505-513. Rolff C. Seasonal variation in δ13C and δ15N of size-fractionated plankton at a coastal station in the northern Baltic proper[J]. Marine Ecology Progress Series, 2000, 203:47-65. 柯志新, 黄良民, 徐军, 等. 大亚湾冬季不同粒级浮游生物的氮稳定同位素特征及其与生物量的关系[J]. 生态学报, 2012, 32(22):7102-7108. Ke Zhixin, Huang Liangmin, Xu Jun, et al. The stable nitrogen isotope of size-fractioned plankton and its relationship with biomass during winter in Daya Bay[J]. Acta Ecologica Sinica, 2012, 32(22):7102-7108. Dehairs F, Rao R G, Mohan P C, et al. Tracing mangrove carbon in suspended matter and aquatic fauna of the Gautami-Godavari Delta, Bay of Bengal (India)[J]. Hydrobiologia, 2000, 431(2/3):225-241. Bannon R O, Roman C T. Using stable isotopes to monitor anthropogenic nitrogen inputs to estuaries[J]. Ecological Applications, 2008, 18(1):22-30. 陈展彦, 武海涛, 王云彪, 等. 基于稳定同位素的湿地食物源判定和食物网构建研究进展[J]. 应用生态学报, 2017, 28(7):2389-2398. Chen Zhanyan, Wu Haitao, Wang Yunbiao, et al. Research progress on food sources and food web structure of wetlands based on stable isotopes[J]. Chinese Journal of Applied Ecology, 2017, 28(7):2389-2398. 李纯厚, 黄洪辉, 林钦, 等. 海水对虾池塘养殖污染物环境负荷量的研究[J]. 农业环境科学学报, 2004, 23(3):545-550. Li Chunhou, Huang Honghui, Lin Qin, et al. Environmental carrying capacity of the pollutants from the prawn seawater culture ponds[J]. Journal of Agro-Environmental Science, 2004, 23(3):545-550. 刘娅琴, 邹国燕, 宋祥甫, 等. 不同营养状态水体中生态浮床对浮游植物群落的影响[J]. 环境科学研究, 2015, 28(4):629-637. Liu Yaqin, Zou Guoyan, Song Xiangfu, et al. Effects of ecological floating bed system(EFBS) on phytoplankton community structure in water bodies with different nutrient levels[J]. Research of Environmental Sciences, 2015, 28(4):629-637.
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