三角褐指藻滤液提取物对东海原甲藻和盐藻生理特性的影响

薛峤娜; 王蕊; 谭丽菊; 王江涛

doi:10.3969/j.issn.0253-4193.2018.04.011

三角褐指藻滤液提取物对东海原甲藻和盐藻生理特性的影响

doi: 10.3969/j.issn.0253-4193.2018.04.011

1.
中国海洋大学海洋化学理论与技术教育部重点实验室, 山东青岛 266100
2.
香港城市大学深圳研究院海洋与人类健康研究中心深圳海洋生物多样性可持续利用重点实验室, 广东深圳 518057

基金项目: 全球变化与海气相互作用专项项目"气候变化对热带大洋上层营养盐供给的影响"（GASI-03-01-02-01）；国家重点研发计划（2016YFC1402101）。

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出版历程
- 收稿日期: 2017-04-18
- 修回日期: 2018-01-15

Effects of the extracts from the filtrate of Phaeodactylum triconutum on Prorocentrum donghaiense and Dunaliella salina analyzed by different physiological parameters

1.
Key Laboratory of Marine Chemistry Theory and Technology/Ministry of Education, Ocean University of China, Qingdao 266100, China
2.
Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity, Research Centre for the Oceans and Human Health, Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China

摘要

摘要: 在实验室培养条件下，利用流式细胞仪从细胞生长、细胞大小、细胞膜完整性、酯酶活性等方面分别研究了三角褐指藻（Phaeodactylum triconutum）滤液提取物对东海原甲藻（Prorocentrum donghaiense）和盐藻（Dunaliella salina）为期6 d生物培养过程中生理特性的影响。结果表明，三角褐指藻滤液乙酸乙酯提取物对东海原甲藻表现出强烈的生长抑制作用，荧光检测显示，其能刺激细胞体积的增大，导致部分藻体细胞膜受损，酯酶活性在短时间内增强，随后受到显著抑制。而提取物对盐藻的生长抑制并不明显，盐藻细胞体积没有受到影响，细胞膜保持了高度完整，酯酶活性在第6天时才受到抑制作用。以上分析表明，东海原甲藻对三角褐指藻滤液乙酸乙酯提取物更加敏感，特殊的细胞内部结构特征有利于化感物质进入东海原甲藻的体内，直接导致藻细胞的死亡。化感物质引起的不同的生理响应中，酯酶活性指标具有更好的敏感性，是在环境压力下生物自我保护的一种应急方式。
- 流式细胞仪 /
- 三角褐指藻滤液提取物 /
- 细胞大小 /
- 细胞膜完整性 /
- 酯酶活性
Abstract: To investigate allelopathic effects of extracts from the filtrate of Phaeodactylum triconutum on growth and physiology of bloom-forming Prorocentrum donghaiense and Dunaliella salina, the cell density, cell size, membrane integrity and esterase activity were determined by the flow cytometry during a 6 d exposure test in laboratory. The results suggested that the growth of P. donghaiense was significantly inhibited by ethyl acetate extracts from the filtrate of P. triconutum. With the ethyl acetate extracts treatments, the cell size increased and the part of the algae membrane integrity was damaged, at the same time esterase activity significantly increased within a short time and was inhibited subsequently. However, the growth of D. salina was not inhibited by extracts from the filtrate of P. triconutum significantly. Both the cell size and membrane integrity was not inhibited, compared with the control cultures, the extracts induced negative effects on the esterase activity after 6 days exposure. These results confirmed that P. donghaiense was more sensitive to the allelochemical contained in the ethyl acetate of P. triconutum. Many P. donghaiense cells rapidly died and disappeared which be caused by the special internal structure of cells that the allelochemicals could easily enter the P. donghaiense cells. Esterase activity was the most sensitive parameter to evaluate the influence of the P. triconutum allelochemical on P. donghaiense, a kind of emergency mode of biological self-protection under environmental pressure.
- flow cytometry /
- the extracts from the filtrate of Phaeodactylum triconutum /
- cell size /
- membrane integrity /
- esterase activity

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参考文献(27)

Anderson D M. Turning back the harmful red tide[J]. Nature, 1997, 388(6642):513-514.

Honjo T. The biology and prediction of representative red tides associated with fish kills in Japan[J]. Reviews in Fisheries Science, 1994, 2(3):225-253.

Horner R A, Garrison D L, Plumley F G. Harmful algal blooms and red tide problems on the US west coast[J]. Limnology and Oceanography, 1997, 42(5):1076-1088.

Granéli E, Weberg M, Salomon P S. Harmful algal blooms of allelopathic microalgal species:The role of eutrophication[J]. Harmful algae, 2008, 8(1):94-102.

Gross E M. Allelopathy of aquatic autotrophs[J]. Critical Reviews in Plant Sciences, 2003, 22(3/4):313-339.

王江涛, 王蕊, 张议文. 海洋微藻释放的他感物质及其研究进展[J]. 海洋环境科学, 2016, 35(3):460-466. Wang Jiangtao, Wang Rui, Zhang Yiwen. Allelochemical released by phytoplankton and the recent progress in marine ecosystem[J]. Marine Environmental Science, 2016, 35(3):460-466.

Yamasaki Y, Shikata T, Nukata A, et al. Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community[J]. The ISME Journal, 2009, 3(7):808-817.

韩秀荣, 高嵩, 侯俊妮, 等. 浒苔干粉末提取物对东海原甲藻和中肋骨条藻的克生作用[J]. 生态学报, 2013, 33(23):7417-7429. Han Xiurong, Gao Song, Hou Junni, et al. Allelopathic effects of extracts from Ulva prolifera powders on the growth of Prorocentrum donghaiense and Skeletonema costatum[J]. Acta Ecologica Sinica, 2013, 33(23):7417-7429.

张议文, 王江涛, 谭丽菊. 旋链角毛藻对中肋骨条藻化感作用的影响因素及化感物质性质初探[J]. 海洋学报, 2014, 36(2):123-129. Zhang Yiwen, Wang Jiangtao, Tan Liju. The study on the influencing factors of Chaetoceros curvisetus allelopathic effect on Skeletonema costatum and the primary research on the properties of Chaetoceros curvisetus allelochemical[J]. Haiyang Xuebao, 2014, 36(2):123-129.

Wang Rui, Wang Jingtao, Xue Qiaona, et al. Preliminary analysis of allelochemicals produced by the diatom Phaeodactylum tricornutum[J]. Chemosphere, 2016, 165:298-303.

Franklin N M, Stauber J L, Lim R P. Development of flow cytometry-based algal bioassays for assessing toxicity of copper in natural waters[J]. Environmental Toxicology and Chemistry, 2001, 20(1):160-170.

Cunningham A, Buonnacorsi G A. Narrow-angle forward light scattering from individual algal cells:implications for size and shape discrimination in flow cytometry[J]. Journal of Plankton Research, 1992, 14(2):223-234.

肖溪. 大麦秸秆对蓝藻化感抑制作用与机理的研究[D]. 杭州:浙江大学, 2012. Xiao Xi. Allelopathic inhibition of Cyanobacteria by barley straw and its mechanism[D]. Hangzhou:Zhejiang University, 2012.

Yu Yang, Kong Fanxiang, Wang Meilin, et al. Determination of short-term copper toxicity in a multispecies microalgal population using flow cytometry[J]. Ecotoxicology and Environmental Safety, 2007, 66(1):49-56.

Zhang Min, Kong Fanxiang, Wu Xiaodong, et al. Different photochemical responses of phytoplankters from the large shallow Taihu Lake of subtropical China in relation to light and mixing[J]. Hydrobiologia, 2008, 603(1):267-278.

于洋, 孔繁翔, 王美林, 等. 应用流式细胞技术研究铜对藻细胞膜完整性及脂酶活性的影响[J]. 应用与环境生物学报, 2006, 12(5):706-709. Yu Yang, Kong Fanxiang, Wang Meilin, et al. Effect of copper on membrane integrality and esterase activity of Microcystis aeruginosa using flow cytometry[J]. Chinese Journal of Applied & Environmental Biology, 2006, 12(5):706-709.

Persidsky M D, Baillie G S. Fluorometric test of cell membrane integrity[J]. Cryobiology, 1977, 14(3):322-331.

Nakai S, Inoue Y, Hosomi M, et al. Growth inhibition of blue-green algae by allelopathic effects of macrophytes[J]. Water Science and Technology, 1999, 39(8):47-53.

Rioboo C, O'Connor J E, Prado R, et al. Cell proliferation alterations in Chlorella cells under stress conditions[J]. Aquatic Toxicology, 2009, 94(3):229-237.

Körner S, Nicklisch A. Allelopathic growth inhibition of selected phytoplankton species by submerged macrophytes[J]. Journal of Phycology, 2002, 38(5):862-871.

Hadjoudja S, Vignoles C, Deluchat V, et al. Short term copper toxicity on Microcystis aeruginosa and Chlorella vulgaris using flow cytometry[J]. Aquatic Toxicology, 2009, 94(4):255-264.

Regel R H, Ferris J M, Ganf G G, et al. Algal esterase activity as a biomeasure of environmental degradation in a freshwater creek[J]. Aquatic Toxicology, 2002, 59(3/4):209-223.

Girling A E, Pascoe D, Janssen C R, et al. Development of methods for evaluating toxicity to freshwater ecosystems[J]. Ecotoxicology and Environmental Safety, 2000, 45(2):148-176.

Lage O M, Sansonetty F, O'Connor J E, et al. Flow cytometric analysis of chronic and acute toxicity of copper (Ⅱ) on the marine dinoflagellate Amphidinium carterae[J]. Cytometry, 2001, 44(3):226-235.

Berglund D L, Eversman S. Flow cytometric measurement of pollutant stresses on algal cells[J]. Cytometry, 1988, 9(2):150-155.

Arsenault G, Popovic R, Cvetkovic A D. Toxic effects of copper on Selenastrum capricornutum measured by a flow cytometry-based method[J]. Water Quality Research Journal of Canada, 1993, 28(4):757-765.

陆斗定, 齐雨藻, Goebel J, 等. 东海原甲藻修订及与相关原甲藻的分类学比较[J]. 应用生态学报, 2003, 14(7):1060-1064. Lu Douding, Qi Yuzao, Goebel J, et al. Redescription of Prorocentrum donghaiense Lu and comparison with relevant Prorocentrum species[J]. Chinese Journal of Applied Ecology, 2003, 14(7):1060-1064.

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