条斑紫菜杂交重组品系(<i>A</i>-18)的筛选与特性分析

江灏; 丁洪昌; 严兴洪

doi:10.3969/j.issn.0253-4193.2018.02.010

条斑紫菜杂交重组品系(A-18)的筛选与特性分析

doi: 10.3969/j.issn.0253-4193.2018.02.010

上海海洋大学水产种质资源发掘与利用教育部重点实验室, 上海 201306;上海海洋大学水产科学国家级实验教学示范中心, 上海 201306;上海海洋大学上海水产养殖工程技术研究中心, 上海 201306

基金项目: 国家高科技研究发展计划（863计划）资助项目（2012AA10A411）；国家自然科学基金资助项目（31072208）；浙江省农业（水产）新品种选育重大科技专项（2016C02055-6）。

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- 收稿日期: 2017-07-10

Selection and characterization of an improved strain(A-18)by hybridization recombinant in Pyropia yezoensis(Bangiales,Rhodophyta)

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China

摘要

摘要: 为筛选出藻体生长快且颜色与野生型色泽相近的条斑紫菜新品系，本研究从条斑紫菜绿色突变体和红色突变体种内杂交产生的后代中，分离出了优良品系A-18。日龄60 d时的叶状体平均长度、长宽比和单株湿质量，A-18品系分别为84.95 cm、49.46和0.52 g，分别是条斑紫菜野生型品系（WT）的3.12、7.01和1.36倍。日龄60 d时叶状体的叶绿素a和总藻胆蛋白含量，A-18品系分别为8.37和53.81 mg/g，均与WT品系较接近。日龄60 d时的叶状体平均厚度，A-18品系仅为20.22 μm，比WT品系降低了29%。另外，A-18品系的壳孢子放散总量为916.01万个/贝壳，是WT品系的1.55倍。综上所述，A-18品系具有生长快、长宽比值大、藻体薄、壳孢子放散量大等优良特性，且藻体颜色与野生型色泽相近，有望在生产中运用。
- 条斑紫菜 /
- 种内杂交 /
- 优良品系 /
- 叶状体 /
- 生长速度 /
- 厚度
Abstract: In order to select a new variety of Pyropia yezoensis with fast growing and wild-type color,following intraspecific hybridization of the green-type pigmentation mutant and red-type pigmentation in P. yezoensis was carried out,and then an improved strain (A-18) was isolated from the recombinants. The mean length,mean length-width ratio and wet weight of 60-day-old blades in A-18 strain were 84.95 cm,49.46 and 0.52 g,which were 3.12,7.01 and 1.36 times those of WT strain. After being cultured for 60 days,the contents of chlorophyll a and phycobiliprotein in A-18 strain were 8.37 and 53.81 mg/g,respectively,both of them were close to the WT strain. The thickness of 60-day-old blades of A-18 strain was 20.22 μm,which was 29% thinner than that of WT strain. In addition,the total number of conchospores released from A-18 strain was 916.01×10⁴ per shell,which was 1.55 times that of WT strain. The above results showed that A-18 strain was characterized by fast growth,large length-width raito,large amount of conchospores,and its color is close to the wild-type,therefore,A-18 strain is expected to be applied in the production.
- Pyropia yezoensis /
- intraspecific hybridization /
- improved strain /
- gametophytic blade /
- growth rate /
- thickness

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参考文献(27)

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