三角褐指藻与抑食金球藻的竞争及化感作用研究

张达娟; 张兴华; 唐学玺; 毕相东; 宋伦; 张树林

doi:10.3969/j.issn.0253-4193.2017.06.009

三角褐指藻与抑食金球藻的竞争及化感作用研究

doi: 10.3969/j.issn.0253-4193.2017.06.009

1.
天津农学院水产学院天津市水产生态及养殖重点实验室, 天津 300384
2.
中国海洋大学海洋生命学院, 山东青岛 266003
3.
天津农学院水产学院天津市水产生态及养殖重点实验室, 天津 300384;南开大学环境科学与工程学院, 天津 300071
4.
辽宁省海洋水产科学研究院, 辽宁大连 116023

基金项目: 国家自然科学基金青年项目（31300393）；中国海洋发展研究会重点项目（CAMAZDA201605）；辽宁省自然科学基金资助项目（2014020182）；天津市科技支撑重点项目（15ZCZDNC00230）；中国博士后科学基金特别资助（2015T80212）；天津市水产生态及养殖重点实验室开放基金项目（TJAE201501）。

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出版历程
- 收稿日期: 2016-09-26
- 修回日期: 2016-12-05

Study on the competition and allelopathy between Phaeodactylum tricornutum and Aureococcus anophagefferens

1.
Department of Fisheries Sciences, Tianjin Agricultural University, Key Labortary of Aqua-ecology and Aquaculture of Tianjin, Tianjin 300384, China
2.
College of Marine Life Science, Ocean University of China, Qingdao 266003, China
3.
Department of Fisheries Sciences, Tianjin Agricultural University, Key Labortary of Aqua-ecology and Aquaculture of Tianjin, Tianjin 300384, China;College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China
4.
Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China

摘要

摘要: 为探明三角褐指藻（Phaeodactylum tricornutum）对抑食金球藻（Aureococcus anophagefferens）是否具有化感作用，本研究以三角褐指藻和抑食金球藻为实验材料，研究了抑食金球藻在单培养和共培养条件下的生长情况及三角褐指藻培养滤液对其生长和叶绿素荧光参数的影响。结果表明：三角褐指藻对抑食金球藻有明显的化感抑制作用。在单培养体系中，抑食金球藻的生长曲线可用逻辑斯谛增长模型拟合，随着起始密度的增加，环境容量（K）逐渐减小，而抑食金球藻的种群瞬间增长率（r）、进入拐点时间及稳定期细胞密度均较为接近；当三角褐指藻与抑食金球藻以不同起始密度比共同培养时，抑食金球藻的生长均受到了显著地抑制（P<0.05），但其抑制作用并未与三角褐指藻的密度呈明显的线性关系；滤液培养实验发现，10 mL和15 mL三角褐指藻培养滤液的加入可对抑食金球藻的生长产生显著影响（P<0.05），对其叶绿素荧光参数没有影响（P>0.05），25 mL和35 mL三角褐指藻培养滤液的加入可对抑食金球藻的生长和叶绿素荧光参数均产生显著的抑制作用（P<0.05），叶绿素荧光参数F_v/F_m、ΦPSⅡ、alpha的值降低，Ik的值增加，PSⅡ受到损害。
- 三角褐指藻 /
- 抑食金球藻 /
- 褐潮 /
- 化感作用
Abstract: To clarify whether Phaeodactylum tricornutum had allelopathy effect on Aureococcus anophagefferens, we studied the interaction between P. tricornutum and A. anophagefferens and the impacts of P. tricornutum culture media filtrate on A. anophagefferens growth and on chlorophyll fluorescence under laboratory conditions. The results show that, P. tricornutum has allelopathy effect on A. anophagefferens. In the mono-culture, the proliferation of A. anophagefferens followed the Logistic model, and its initial cell density affected significantly the environmental-carrying capacity (K) of algal populations; and K values decreased gradually with the increase of initial cell densities.In the co-culture, the growth of A. anophagefferens was inhibited significantly (P<0.05) by P. tricornutum of different algal initial cell densities, but the inhibitory effect changed indistinctively with the increase of P. tricornutum proportion. In the filtrate cultivation experiment of A. anophagefferens, we found that: P. tricornutum filtrate at 10 mL or 15 mL could remarkably inhibite the growth of A. anophagefferens (P<0.05) with no effect on its chlorophyll fluorescence; P. tricornutum filtrate at 25 mL and 35 mL could cause PSⅡ irreversible damage (F_v/F_m, ΦPSⅡ, alpha, NPQ values reduced) and death of A. anophagefferens.
- Phaeodactylum tricornutum /
- Aureococcus anophagefferens /
- brown tide /
- allelopathy

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