泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析

任付真; 姚韩韩; 董迎辉; 周小龙; 林志华

doi:10.3969/j.issn.0253-4193.2016.08.008

泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析

doi: 10.3969/j.issn.0253-4193.2016.08.008

1.
上海海洋大学水产与生命学院, 上海 201306;浙江万里学院生物与环境学院浙江省水产种质资源高效利用技术研究重点实验室, 浙江宁波 315100
2.
浙江万里学院生物与环境学院浙江省水产种质资源高效利用技术研究重点实验室, 浙江宁波 315100
3.
上海海洋大学水产与生命学院, 上海 201306

基金项目: 国家现代贝类产业技术体系项目（CARS-48）；浙江省自然科学基金重点项目（LZ12C19001）；浙江省重大科技专项（2012C12907-4）；国家水产种质资源平台项目（2015DKA30470）。

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- 收稿日期: 2015-10-23
- 修回日期: 2016-04-06

cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa

1.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
2.
Key Laboratory of Aquatic Germplasm Resource of Zhejiang, College of Biological & Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China
3.
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

摘要

摘要: HDAC1作为HDACs家族中重要成员，可使组蛋白去乙酰化进而调节基因表达，在细胞分化和胚胎早期发育中起重要作用。本文利用SMART RACE技术克隆得到泥蚶HDAC1(Tg-HDAC1)基因的cDNA全长序列，并对其内含子进行扩增及不同组织、不同发育时期的定量表达。结果发现：Tg-HDAC1基因的cDNA序列全长为2275 bp，开放阅读框(ORF)1587 bp，编码528个氨基酸；Tg-HDAC1蛋白序列与斑马鱼、鸡、小鼠等的相似性都在80%以上，表明该蛋白氨基酸序列在物种进化过程中具有保守性；在Tg-HDAC1基因中扩增出13个内含子，均存在于开放阅读框中，且都遵循GT-AG原则；荧光定量PCR(qRT-PCR)分析结果显示，Tg-HDAC1基因在成体血液、内脏团、外套膜、鳃、斧足和闭壳肌6个组织中均有表达，而在足中的表达量最高，且与其余5组织中的表达量有极显著差异(P<0.01)，说明它对该组织生长具有重要作用。不同发育时期的表达差异结果表明，Tg-HDAC1基因在担轮幼虫期表达量最高，显著高于其他发育时期(P<0.05)。
- 泥蚶 /
- 组蛋白去乙酰化酶1(HDAC1) /
- 基因克隆 /
- 内含子 /
- 表达分析
Abstract: As an important member of HDACs family, HDAC1 can regulate gene expresstion and play a crucial role in cell differentiation and early embryonic development. Tg-HDAC1 cDNA was cloned by SMART RACE technique and then the bioinformatics, expression analysis, and intron amplification of Tg-HDAC1 were carried out in Tegillarca granosa. The full length of Tg-HDAC1 cDNA was 2 275 bp, containing a complete 1587 bp ORF encoding 528 amino acids. The homologous similarity between the blood clam and other species, such as Danio rerio, Gallus gallus, Mus musculus, was more than 80%, which indicate that HDAC1 is relatively conserved in the evolution. Thirteen introns of Tg-HDAC1 were amplified in ORF, which all of them follow the principle of GT-AG. The results of six tissue-specific expression by real time PCR showed that Tg-HDAC1 gene expressed in all tissues, and the expression of foot were significantly higher than other tissues(P<0.01), which suggest that the gene play an important role in the course of foot growth. The relative expression in different stages revealed that the expression of Tg-HDAC1 gradually increased with the process of the development, and showed the highest in trochophore stage (P<0.05).
- Tegillarca granosa /
- HDAC1 /
- gene cloning /
- intron /
- expression analysis

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参考文献(30)

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泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析

doi: 10.3969/j.issn.0253-4193.2016.08.008

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cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa

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泥蚶HDAC1基因cDNA全长、内含子克隆及时空表达特征分析

doi: 10.3969/j.issn.0253-4193.2016.08.008

计量

出版历程

cDNA, introns cloning and spatiotemporal expression analysis of HDAC1 gene in Tegillarca granosa

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出版历程

目录