渤海沉积环境中细菌汞还原酶基因<em>merA</em>的多样性分析

何培青; 李力; 刘季花; 方习生

doi:10.3969/j.issn.0253-4193.2015.08.010

渤海沉积环境中细菌汞还原酶基因merA的多样性分析

doi: 10.3969/j.issn.0253-4193.2015.08.010

1.
国家海洋局第一海洋研究所海洋生态研究中心, 山东青岛 266061
2.
国家海洋局第一海洋研究所海洋沉积与环境地质国家海洋局重点实验室, 山东青岛 266061

基金项目: 海洋公益性行业科研专项(201105003)。

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出版历程
- 收稿日期: 2014-12-07
- 修回日期: 2015-06-02

Diversity of mercuric reductase genemerA in Bohai Sea sediment

1.
Marine Ecology Research Center, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Key laboratory of State Oceanic Administration for Marine Sedimentology & Environmental Geology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 细菌汞还原酶MerA对环境中的汞具有潜在的还原和解毒作用。本研究对2011-2012年采自渤海湾天津滨海新区大沽河排污河口、渤海湾近岸及莱州湾垦利养殖区的表层沉积物,进行了放线菌门、厚壁菌门、α-变形菌纲和β/γ-变形菌纲汞还原酶基因merA克隆文库的构建和序列测定,并对merA基因序列编码的MerA蛋白序列进行分析。结果表明:渤海沉积环境中发育多样性丰富的MerA,排污河口和养殖区中厚壁菌门、α-变形菌纲和α/β/γ-变形菌纲的MerA多样性均高于渤海湾近岸的。3个区域具有不同的MerA组成特征,厚壁菌门中与Bacillus sp. MB2021亲缘关系最近的MerA的总丰度在养殖区最高(24.3%),与Paenisporosarcina sp. TG20亲缘关系最近的MerA的总丰度在排污河口最高(46.3%)。不同环境背景、外源merA基因输入、汞等重金属污染物的联合选择可能导致了渤海沉积环境中MerA多样性和组成的变化。
- 渤海 /
- 排污河口 /
- 养殖区 /
- 汞还原酶 /
- 多样性
Abstract: Mercuric reductase gene merA had a potential role in mercuric reduction and detoxification in environment. During 2011-2012, surface sediments were collected from wastewater discharge of Dagu River in Tianjin Binhai New Area from Bohai Bay, near shore of Bohai Bay and Kenli aquaculture facility from Laizhou Bay. Clone libraries of merA from Actinomycete, Firmicutes, α-Proteobacteria and β/γ-Proteobacteria were constructed and the sequences were determined. Deduced MerA protein sequences of merA gene were then analyzed. The results showed that Bohai Sea sedimentary environment contained high diversity of MerA, and MerA diversity within Firmicutes, α-Proteobacteria and α/β/γ-Proteobacteria from wastewater discharge and aquaculture facility were higher than that in near shore of Bohai Bay. The MerA composition in different locations also differed, and within Firmicutes, the highest total abundance of MerAs that had the highest similarity with Bacillus sp. MB2021 was found in aquaculture facility(24.3%), while, the highest total abundance of MerAs that had the highest similarity with Paenisporosarcina sp. TG20 was found in wastewater discharge(46.3%). Different environment context, exogenous merA gene input, and co-selection caused by mercury and other heavy metal pollutants might together lead to the change of merA diversity, community composition in Bohai Sea sedimentary environment.
- Bohai Sea /
- wastewater discharge /
- aquaculture facility /
- mercuric reductase /
- diversity

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参考文献(23)

Philbert M A,Billingsley M L,Reuhl K R. Mechanisms of injury in the central nervous system[J]. Toxicologic Pathology,2000,28(1): 43-53.

Pirrone N,Cinnirella S,Feng Xinbin,et al. Global mercury emissions to the atmosphere from anthropogenic and natural sources[J]. Atmospheric Chemistry and Physics,2010,10: 5951-5964.

Eto K. Minamata disease[J]. Neuropathology,2000,20(S1): S14-S19.

Tomiyasu T,Matsuyama A,Eguchi T,et al. Spatial variations of mercury in sediment of Minamata Bay,Japan[J]. Science of the Total Environment,2006,368(1): 283-290.

Han F X,Su Yi,Monts D L,et al. Binding,distribution,and plant uptake of mercury in a soil from Oak Ridge,Tennessee,USA[J]. Science of the Total Environment,2006,368(2/3): 753-768.

Lin C C,Yee N,Barkay T. Microbial transformation in the mercury cycle[M]// Liu Guangliang,Cai Yong,O'Driscoll N. Environmental Chemistry and Toxicology of Mercury. Hoboken,N J: John Wiley & Sons,Inc.,2012: 155-191.

Watras C J,Back R C,Halvorsen S,et al. Bioaccumulation of mercury in pelagic freshwater food webs[J]. Science of the Total Environment,1998,219(2/3): 183-208.

Boyd E S,King S,Tomber-line J K,et al. Methylmercury enters an aquatic food web through acidophilic microbial mats in Yellowstone National Park,Wyoming[J]. Environmental Microbiology,2009,11(4): 950-959.

Rimmer C C,Miller E K,McFarland K P,et al. Mercury bioaccumulation and trophic transfer in the terrestrial food web of a montane forest[J]. Ecotoxicology,2010,19(4): 697-709.

Clarkson T W,Magos L. The toxicology of mercury and its chemical compounds[J]. Critical Reviews in Toxicology,2006,36(8): 609-662.

Van Oostdam J,Donaldson S G,Feeley M,et al. Human health implications of environmental contaminants in Arctic Canada: a review[J]. Science of the Total Environment,2005,351/352: 165-246.

Dietz R,Outridge P M,Hobson K A. Anthropogenic contributions to mercury levels in present-day Arctic animals——a review[J]. Science of the Total Environment,2009,407(24): 6120-6131.

Barkay T,Miller S M,Summers A O. Bacterial mercury resistance from atoms to ecosystems[J]. FEMS Microbiology Reviews,2003,27(2/3): 355-384.

Schelert J,Dixit V,Hoang V,et al. Occurrence and characterization of mercury resistance in the hyperthermophilic archaeon Sulfolobus solfataricus by use of gene disruption[J]. Journal of Bacteriology,2004,186(2): 427-437.

Osborn A M,Bruce K D,Strike P,et al. Distribution,diversity and evolution of the bacterial mercury resistance (mer) operon[J]. FEMS Microbiology Reviews,1997,19(4): 239-262.

Ramaiah N,De J. Unusual rise in mercury-resistant bacteria in coastal environs[J]. Microbial Ecology,2003,45(4): 444-454.

Oregaard G,Sørensen S J. High diversity of bacterial mercuric reductase genes from surface and sub-surface floodplain soil (Oak Ridge,USA)[J]. The ISME Journal,2007,1: 453-467.

Møller A K,Barkay T,Al-Soud W A,et al. Diversity and characterization of merc極瑲獹?慲湥摳?敳獴瑡楮浴愠瑢楡湣杴?獲灩敡挠楩敮猠?牮楯捷栬湦敲獥獳孨?嵡???瀠灡汮楤攠摳?慡渭摩??渠癢楲物潮湥洠敦湲瑯慭氠??楥挠版潩执楨漠汁潲杣祴??せお???????????ひ????ぬ???戠牅?孯??嵧?刬′?攱瘱攬氷漵瀨洳攩渺琠″?漰爭攴‰吱攮愼浢?￣剛??慝?汐慩湴杫甦愣朲攲?愻湮摥?攠湌瘠楋爬潔湡浭敭湩瑮?普漠牍?獈瑹慮瑮楩獮瑥楮挠慁氬?捴漠浡灬甮琠楆湩杳孨?嵦??噭楩敮湧渠慡??剥??潳甠湴摨慥琠楡潢湵?晤潡牮?卥琠慡瑮楤猠瑤楩捶慥汲??潴浹瀠畯瑦椠湴杨?㈠ねづ???扲特?孲??嵩?婴楡湮杣敥爠????洠愼牥慭氾?婥敲瑁琠氼支牥??????畡桲物浮慥渠?????敥瑮?慳汛???氠潍扩慣汲?灢慥瑳琠敡牮湤猠?潮晶?扲慯据瑭敥牮楴慳氬′戰攱琱愬?搶椨瘳攩爺猠椲琰礵?椲渱?献攼慢晲氾潛漲爰?愠湒摡?獯敮慤眠慊琠敂爬?敥捲潴獨祥猠瑔攬浌獡学?嵴??偒?潥却?佡湬攮???ぬ?????????敳㈠???ぷ??扮爠?孹??嵯??敤湩瑭敥???偲楹攠牰捲敯?????即愠湡瑮潤猠?????敲瑥?慣汥???映晭敥捲瑣?潲晹?晲敥敳摩?慴湡摮?映敢敡摣楴湥杲?楡渠?琼桥敭￣捭略汲瑁甼爯敥?漾昩?獩慮氠浥潳湴極摡獲?漠湭?瑤桦敬?浴慳爠椨湓敥?慮煥甬慆瑲楡据?敥温癛楊牝漮渠浍敡湲瑩??愠?獯祬湬瑵桴敩獯楮猠?晵潬牬??畩牮漬瀲攰愰游?愵然用愶挩町氠琱由父攸嬭?崱???焼畢慲挾畛氲琱畝爠敎…?渲琳攷爻渠慃瑨楡潤湨慡汩?㈠こ???????????????水至?ane S,et al. Analysis of mercuric reductase (merA) diversity in an anaerobic mercury-contaminated sediment enrichment[J]. Environmental Microbiology,2006,8(10): 1746-1752.

徐亚岩,宋金明,李学刚,等. 渤海湾表层沉积物各形态重金属的分布特征与生态风险评价[J]. 环境科学,2012,33(3): 732-740. Xu Yayan,Song Jinming,Li Xuegang,et al. Variation characteristics and potential ecological risk assessment of heavy metals in the surface sediments of Bohai Bay[J]. Environmental Science,2012,33(3): 732-740.

林曼曼,张勇,薛春汀,等. 环渤海海域沉积物重金属分布特征及生态环境评价[J]. 海洋地质与第四纪地质,2014,33(6): 41-46. Lin Manman,ZhangYong,Xue Chunting,et al. Distribution pattern of heavy metals in the surface sediments of the area of Circum-Bohai Bay and ecological environment assessment[J]. Marine Geology & Quaternary Geology,2014,33(6): 41-46.

刘文新,邱炜珣,陈江麟. 近年渤海与黄海北部沿岸底栖贝类体内微污染物的分布特征[J]. 环境科学,2011,32(2): 445-451. Liu Wenxin,Qiu Weixun,Chen Jianglin. Distribution characteristics of trace pollutants in benthic mussels from the coastal areas of Bohai Sea and North Yellow Sea[J]. Environmental Science,2011,32(2): 445-451.

Schaefer J K,Yagi J,Reinfelder J R,et al. Role of the bacterial organomercury lyase (MerB) in controlling methylmercury accumulation in mercury-contaminated natural waters[J]. Environmental Science & Technology,2004,38(16): 4304-4311.

Schloss P D,Handelsman J. Introducing DOTUR,a computer program for defining operational taxonomic un

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