不同培养方式及换水频率对坛紫菜(<em>Porphyra haitanensis</em>)自由丝状体生长及藻胆蛋白含量的影响

丁兰平; 颜泽伟; 张全亮; 徐佩杭; 黄冰心

doi:10.3969/j.issn.0253-4193.2015.06.012

不同培养方式及换水频率对坛紫菜(Porphyra haitanensis)自由丝状体生长及藻胆蛋白含量的影响

doi: 10.3969/j.issn.0253-4193.2015.06.012

汕头大学海洋生物研究所, 广东汕头 515063

基金项目: 国家科技支撑项目(2012BAC07B05);国家自然科学基金(31270257);广东省科技计划项目(2011B031100010,2012A020200007);广东省高等学校人才引进项目(2011年);汕头市科技计划项目(2012-171)。

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- 收稿日期: 2014-07-31

Influence of cultivation methods and the frequency of the medium on the growth and phycobiliprotein content of free living conchocelis of Porphyra haitanensis

1. Marine Biology Institute, Shantou University, Shantou 515063, China

摘要

摘要: 本实验以坛紫菜自由丝状体为材料,研究不同培养方式(静置、摇床、通气)和换水频率(5 d、7 d、10 d、20 d)对丝状体生长及藻胆蛋白(PE、PC)含量变化的影响。研究结果表明:坛紫菜自由丝状体在摇床培养条件下生长较好,特定生长速率可达10.832 7%~10.891 5%;摇床培养下的丝状体PE含量相对静置培养的较高一些,通气培养的相对较低,最高为36.816 8 mg/g;静置条件下培养的丝状体PC含量最高,摇床培养次之,通气培养最低,最低为1.082 4 mg/g。相同培养方式下,换水频率对丝状体特定生长速率影响不大;除通气培养外,换水频率20 d其PE、PC含量较高。方差分析结果表明:实验范围内的培养方式及换水频率对丝状体特定生长速率大小、PE和PC含量不存在交互作用。本文实验结果对坛紫菜自由丝状体产业化应用于食品加工、色素提取等产业提供了实验理论和依据。
- 丝状体 /
- 特定生长率 /
- 藻红蛋白 /
- 藻蓝蛋白 /
- 培养方式 /
- 换水频率
Abstract: The cultivation methods (standing, rocking, ventilating) and the frequency in changing water (once in 5 d, 7 d, 10 d, 20 d ) were studied on influence of the growth and phycobiliprotein (PE, PC) content of free living Conchocelis of Porphyra haitanensis. The results show that under the condition of rocking, its growth is better, which SGR can reach 10.832 7%-10.891 5%; and its PE content is higher than that of under the condition of standing but lower than that of under the condition of ventilating, and the highest value is 36.816 8 mg/g. Under standing, the PC content reaches the highest, which higher than that of under rocking, and it reaches the lowest under ventilating with value 1.082 4 mg/g. Under the same culture way, the frequency in changing water does not much affect the SGR of free living Conchocelis. However, when the frequency is once in 20 d, its PE and PC contents are higher than other frequency except those of under the condition of ventilating. The ANOVA results show that interaction between the culture methods and water changing frequency does not exist on the SGR, PE and PC content of the conchocelis. It provides an experimental and theoretical basis for free living Conchocelis of Porphyra haitanensis applying to industrial application in food processing and pigment picking industry.
- conchocelis /
- SGR /
- PE /
- PC /
- culture methods /
- frequency of the medium

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