伶鼬榧螺(<em>Oliva mustelina</em>)的分子鉴定及其形态变异

李海涛; 何薇; 周鹏; 陈凯彪; 董燕红

doi:10.3969/j.issn.0253-4193.2015.04.011

伶鼬榧螺(Oliva mustelina)的分子鉴定及其形态变异

doi: 10.3969/j.issn.0253-4193.2015.04.011

国家海洋局南海环境监测中心, 广东广州 510300

基金项目: 海洋公益性行业科研专项(201305010,201105003);南海区海洋环境质量综合评价方法(DOMEP(MEA)-01-03).

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出版历程
- 收稿日期: 2014-06-04
- 修回日期: 2015-01-03

Molecular identification of Oliva mustelina and its morphological variation

South China Sea Environmental Monitoring Center, State Oceanic Administration, Guangzhou 510300, China

摘要

摘要: 根据榧螺螺旋部的差异可将其分为两种不同的形态类型,类型Ⅰ(Morphotype Ⅰ)的个体螺旋部陷入体螺层中,其壳顶与前部数螺层愈合;类型Ⅱ(Morphotype Ⅱ)的个体螺旋部高出壳顶,缝合线明显,此类型可明确鉴定为伶鼬榧螺(Oliva mustelina).本研究通过线粒体COⅠ和16S rRNA基因序列的分析对两种形态类型的榧螺进行了分子鉴定.结果表明:两种形态类型的榧螺含有共享的单倍型;不同形态类型间的遗传距离和同一形态类型内的遗传距离重叠;两者的单系性在系统发育分析中没有得到显现,而是共同构成一个单系.因此,两种形态类型的个体均为伶鼬榧螺.类型Ⅰ可能是伶鼬榧螺中一种少见的有别于典型个体的形态变异类型.
- 伶鼬榧螺 /
- COⅠ基因 /
- 16SrRNA基因 /
- 形态变异
Abstract: Based on the morphologic difference of spire,Oliva snails with similar shell colors and patterns can be divided into two morphotypes. Morphotype Ⅰ is characterized by the sunk spire in its concavity below the shoulder of the body whorl and the whorls of spire fused together. Morphotype Ⅱ shows the identical conchological characteristics of typical specimens of O. mustelina. The mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) and 16S rRNA gene segments of these two morphotypes were sequenced. Genetic analyses clearly showed many common haplotypes and lack of significant genetic differentiation between the two morphotypes. In addition,further phylogenetic analyses showed that all haplotypes were grouped in a monophyletic clade. Thus,the two morphotypes are not genetically diagnosable,and therefore should be assigned to species O. mustelina. Morphotype Ⅰ may be a new form of O. mustelina that differ from the typical specimens.
- Oliva mustelina /
- COⅠ gene /
- 16S rRNA gene /
- morphological variation

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