牙鲆选育F3代家系的建立及遗传效应分析

田永胜; 陈红林; 齐文山; 姜静; 张英平; 刘万军; 李宝玉; 刘寿堂; 孙德强; 陈松林

doi:10.3969/j.issn.0253-4193.2015.04.004

牙鲆选育F3代家系的建立及遗传效应分析

doi: 10.3969/j.issn.0253-4193.2015.04.004

1.
中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室, 山东青岛 266071
2.
中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室, 山东青岛 266071;上海海洋大学水产与生命学院, 上海 201306
3.
中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室, 山东青岛 266071;中国海洋大学海洋生命学院, 山东青岛 266003
4.
海阳市黄海水产有限公司, 山东海阳 265100

基金项目: "863"高技术研究发展计划(2012AA10A408);国家自然科学基金(31372510);上海海洋大学研究生科研基金(A1-0209-14-0900-37);山东省泰山学者建设工程专项资助.

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出版历程
- 收稿日期: 2014-07-15
- 修回日期: 2014-12-17

Establishment and estimated genetic effects of Paralichthys olivaceus F3 families

1.
Key Laboratory for Sustainable Utilization of Marine Fishery Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
2.
Key Laboratory for Sustainable Utilization of Marine Fishery Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
3.
Key Laboratory for Sustainable Utilization of Marine Fishery Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Ocean University of China, Qingdao 266003, China
4.
Huanghai Aquaculture Ltd., Haiyang 265100, China

摘要

摘要: 牙鲆是太平洋西岸海水养殖、捕捞和自然增殖的主要鱼类,但是人工养殖病害多、生长慢,针对这一问题,从2003年开始开展了牙鲆选择育种.本文利用筛选和培育的F1、F2代牙鲆家系核心群体,构建了F3代家系52个,在生长到196 d时,对家系1 560尾鱼苗全长、体宽、体质量、全长日增量和体质量日增量5个生长性状进行测量和计算,获得7 800个数据.采用"加性－显性"线性模型、最小范数二阶无偏估计法(MINQUE)对以上生长性状的边际平均值、加性方差分量、显性方差分量、随机方差分量、狭义遗传力、广义遗传力、亲本加性效应、杂交随机效应等遗传参数进行了评估.结果显示:1202、1206、1226和1211家系以上生长性状的边际平均值同时显著高于其他家系.以上5个生长性状的加性方差分量比率为(0.099±0.01)~(0.24±0.02),显性方差分量比率为(0.30±0.02)~(0.31±0.02),随机方差分量比率为(0.48±0.02)~(0.61±0.01),5个性状狭义遗传力为0.099~0.24,广义遗传力为0.38~0.54,以上遗传参数均达极显著性水平(p< 0.01).综合比较18个亲本5个性状的加性效应预测值,发现F0917、F0908、F0905、F0915、F09125和KS(韩国牙鲆)亲本在5个性状上都具有极显著的正向效应(p< 0.01),其他亲本5个性状都不同程度的表现为显著(p< 0.05)或极显著(p< 0.01)的负向加性效应.对42个杂交组合显性随机效应进行预测,D(2×6)、D(2×18)、D(3×4)、D(7×12)、D(7×14)和D(8×13) 6个杂交组合在5个性状上都表现为极显著(p< 0.05)正效应,利用这些杂交组合建立家系将促进牙鲆以上5个性状的生长.本文研究结果,为牙鲆优良家系的建立及新品种的培育初步筛选出了性状优良的亲本、杂交组合和家系,同时为牙鲆进一步选择育种提供了理论和技术依据.
- 牙鲆 /
- F3代家系 /
- 遗传力 /
- 加性效应 /
- 显性效应
Abstract: Paralichthys olivaceus is the major species for marine aquaculture and fisheries on the western Pacific coast. Due to the high disease incidence and slow growth rate observed in artificial-breeding programs,the selective breeding of Japanese flounder has been pursued since the beginning of this century. Here,we established 52 F3 families based on the screened F1 and F2 core families that we bred previously. Approximately 196 days after hatching,we measured five growth traits of 1 560 fry from the 52 families,for a total of 7 800 data points. An "A-D" model and MINQUE were used to estimate the marginal mean,additive-variance component,dominance-variance component,random-variance component,narrow-sense heritability,broad-sense heritability,parental additive effect and random hybridization effect of each trait. The additive-variance component ratios of the five traits were between (0.099±0.01) and (0.24±0.02); the dominance-variance component ratios were between (0.30±0.02) and (0.31±0.02); the random-variance component ratios were between (0.48±0.02) and (0.61±0.01); the narrow-sense heritabilities were between 0.099 and 0.24; and the broad-sense heritabilities were between 0.38 and 0.54. Each of these genetic parameters showed highly significant differences (p <0.01). The random dominance effects of 42 crosses were predicted,and D(2×6),D(2×18),D(3×4),D(7×12),D(7×14) and D(8×13) all showed highly significant positive effects (p <0.01) on the five traits. The results of this study provide a preliminary set of optimal parental lines,crosses and families to establish improved Japanese flounder strains. These results also provide a theoretical and technical foundation for further selective breeding.
- japanese flounder /
- Paralichthys olivaceus /
- F3 family /
- heritability /
- additive effect

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参考文献(35)

杨正勇,王春晓. 全球视野下中国鲆鲽类养殖业的发展[J]. 中国渔业经济,2009,6(27):115-122. Yang Zhengyong,Wang Chunxiao. On the development of flatfish aquaculture industry of China:a global perspective[J]. Chinese Fisheries Economics,2009,6(27):115-122.

田永胜,陈松林,徐田军,等. 牙鲆不同家系生长性能比较及优良亲本选择[J]. 水产学报,2009,33(6):901-912. Tian Yongsheng,Chen Songlin,Xu Tianjun,et al. The comparison of growth performances of Japanese flounder (Paralichthys olivaceus) families and selection of parents with good trait[J].Journal of Fisheries of China,2009,33(6):901-912.

Liu Y G,Chen S L,Li B F. Genetic differentiation among common and selected hatchery populations of flounder: Evidence from RAPD markers[J]. Biochemical Systematics and Ecology,2007,35: 689-695.

刘海金,朱晓琛,孙效文,等. 牙鲆5个养殖群体的遗传多样性分析[J]. 中国水产科学,2008,15(1):30-37. Liu Haijin,Zhu Xiaoshen,Sun Xiaowen,et al. Analysis of genetic structure among 5 cultured stocks of Japanese flounder Paralichthys olivaceus[J]. Journal of Fishery Sciences of China,2008,15(1):30-37.

Zhang Y X,Chen S L,Liu Y G,et al. Major Histocompatibility Complex Class IIB Allele Polymorphism and Its Association with Resistance/Susceptibility to Vibrio anguillarum in Japanese Flounder (Paralichthys olivaceus) [J]. Marine Biotechnology,2006,8:600-610.

Xu T J,Chen S L. Genomic structure of DAA gene and polymorphism within MHC-DAA alleles in Japanese flounder (Paralichthys olivaceus) [J]. Yi Chuan,2009,31: 1020-1028.

Song W,Pang R,Niu Y,et al. Construction of high-hensity genetic linkage maps and mapping of growth-related quantitative trail loci in the Japanese flounder (Paralichthys olivaceus) [J]. PLoS One,2012,7(11): e50404.

Tian Y S,Xu T J,Liang Y,et al. Estimates of genetic and phenotypic parameters for weight and length in Paralichthys olivaceus(Temminck et Schlegel) [J]. Acta Oceanology Sinica,2011,30(6):58-64.

田永胜,徐田军,陈松林,等.三个牙鲆育种群体亲本效应及遗传参数估计[J].海洋学报,2009,31(6):119-128. Tian Yongshen,Xu Tianjun,Chen Songlin,et al.Parent effects and estimation of genetic parameters for three Japanese flounder breeding populations[J]. Haiyang Xuebao,2009,31(6):119-128.

Zhu X P,You F,Zhang P J,et al. Effects of cold shock on microtubule organization and cell cycle in gynogenetically activated eggs of olive flounder (Paralichthys olivaceus) [J]. Mar Biotechnol (NY),2006,8: 312-318.

刘海金,陆桂,王晓梅,等. 有丝分裂雌核发育牙鲆的微卫星鉴定[J].中国水产科学,2010,17(5):889-894. Liu Haijin,Lu Gui,Wang Xiaomei,et al. Identification of mitogynogenetic Japanese flounder (Paralichthys olivaceus) using microsatellite marker[J]. Journal of Fishery Sciences of China,2010,17(5):889-894.

陈松林,田永胜,徐田军,等.牙鲆抗病群体和家系的建立及其生长和抗病性能初步测定[J]. 水产学报,2008,32(5):665-674. Chen Songlin,Tian Yongsheng,Xu Tianjun,et al. Development and characterization for growth rate and disease resistance of disease-resistance population and family in Japanese flounder (Paralichthys olivaceus) [J].Journal of Fisheries of China,2008,32(5): 665-674.

田永胜,陈松林,徐田军,等. 牙鲆不同家系生长性能比较及优良亲本选择[J]. 水产学报,2009,33(6):901-912. Tian Yongshen,Chen Songlin,Xu Tianjun,et al. The comparison of growth performances of Japanese flounder (Paralichthys olivacus) families and selecion of parents with good trait[J]. Journal of Fisheries of China,2009,33(6):901-912.

全国水产技术推广总站. 2010水产新品种推广指南[M]. 北京:中国农业出版社,2011:242-268. National Fisheries Extension Center. 2010 Promotion of new varieties of aquatic guide[M]. Beijing: Agriculture Press of China,2011:242-268.

Sakamoto T,Okamoto N. Marker-assisted breeding of a lymphocystis disease-resistant Japanese flounder (Paralichthys olivaceus) [J]. Aquaculture ,2007,272:291-295.

朱军.遗传模型分析方法[M].北京:中国农业出版社,1997:58-87. Zhu Jun. Analysis method of genetic model[M].Beijing:Agriculture Press of China,1997:58-87.

盛志廉. 数量遗传学[M]. 北京: 科学出版社,1999. Sheng Zhilian. Quantitative genetics[M]. Beijing: Science Press,1999.

田永胜,陈松林,严安生,等.牙鲆的胚胎发育[J].水产学报,2004,28(6):609-616. Tian Yongsheng,Chen Songlin,Yan Ansheng,et al. Study on the embryonic development of Paralichthys olivaceus[J]. Journal of Fisheries of China,2004,28(6):609-616.

翟虎渠,王建康.应用数量遗传[M].北京:中国农业科学技术出版社,2007:1-250. Zhai Huqu,Wang Jiankang. Application of quantitative genetics[M]. Beijing: Agricultural Science and Technology Press of China,2007:1-250.

杨泽明,熊远著,喻传洲.影响猪遗传评估效果的主要因素研究[J].华中农业大学学报,2001,20(6):598-602. Yang Zeming,Xiong Yuanzhu,Yu Chuanzhou. The development of pig genetic evaluation[J]. Journal of Huazhong Agricultural University,2001,20(6):598-602.

Neira R,Diaz N F,Gall G A E,et al. Genetic improvement in coho salmon (Oncorhynchus kisutch): Ⅰ. Selection response and inbreeding depression on harvest weight[J]. Aquaculture,2006,257: 9-17.

Su G S,Liljedahl L E,Gall G A E. Genetic correlations between body weight at different ages and with reproductive traits in rainbow trout[J]. Aquaculture,2002,213: 85-94.

Eknath A E,Bentsen H B,Ponzoni R W,et al. Genetic improvement of farmed tilapias: Composition and genetic parameters of a synthetic base population of Oreochromis niloticus for selective breeding[J]. Aquaculture,2007,273: 1-14.

蔡一林,岳永生.水产生物统计[M]. 北京:中国农业出版社,2004. Cai Yilin,Yue Yongshen. Aquatic biostatistics[M]. Beijing: Agriculture Press of China ,2004.

栾生,孔杰,王清印.水产动物育种值估计方法及其应用的研究进展[J].海洋水产研究. 2008,29(3):101-108. Luan Sheng,Kongjie,Wang Qingyin. Methods and application of aquatic animal breeding value estimation: A review[J]. Marine Fisheries Research,2008,29(3):101-108.

马爱军,王新安,雷霁霖.大菱鲆(Scophthalmus maximus)不同生长阶段体重的遗传参数和育种值估计[J]. 海洋与湖泊,2009,40(2):187-194. Ma Aijun,Wang Xin'an,Lei Jilin.Genetic parameterization for turbot Scophthalmus maximus: implication to breeding strategy[J].Oceanologia et Limnologia Sinica,2009,40(2):187-194.

王炳谦,刘宗岳,高会江,等.应用重复力模型估计虹鳟生长性状的遗传力和育种值[J]. 水产学报,2009,33(2):182-188. Wang Bingqian,Liu Zongyue,Gao Huijiang,et al. Estimation of genetic parameters for growth traits in rainbow trout (Oncorhynchus mykiss) with differentmodels[J]. Journal of Fisheries China,2009,33(2):182-188.

Nielsen H M,Odegard J,Olesen I,et al. Genetic analysis of common carp (Cyprinus carpio) strains:Ⅰ. Genetic parameters and heterosis for growth traits and survival[J]. Aquaculture,2010,304:14-21.

王晓清,王志勇,何湘蓉. 大黄鱼40 日龄体长和体重遗传力估计[J]. 集美大学学报,2010,15(1):7-10. Wang Xiaoqing,Wang Zhiyong,He Xiangrong. Heritability estimate of 40-day-old body length and weight of Pseudosciaena crocea[J].Journal of Jimei University(Natural Science),2010,15(1):7-10.

Thodesen J,Rye M,Wang Y X,et al. Genetic improvement of tilapias in China: Genetic parameters and selection responses in growth of Nile tilapia (Oreochromis niloticus) after six generations of multi-trait selection for growth and fillet yield[J]. Aquaculture,2011,(322/323):51-64.

Bentsen H B,Gjerde B,Nguyen N H,et al. Genetic improvement of farmed tilapias: Genetic parameters for body weight at harvest in Nile tilapia (Oreochromis niloticus) during five generations of testing in multiple environments[J]. Aquaculture,2012,(338/341): 56-65.

Liu Y X,Wang G X,Wang Y F,et al. Estimation of genetic parameters for growth traits of Japanese flounder Paralichthys olivaceus using an animal model[J]. Fisheries Science, 2011,77: 87-93.

Pante M J R,Gjerde B,McMillan I. Effect of inbreeding on body weight at harvest in rainbow trout,Oncorhynchus mykiss[J]. Aquaculture,2001,192:201-211.

Pante M J R,Gjerde B,McMillan I. Ignacy Misztal.Estimation of additive and dominance genetic variances for body weight at harvest in rainbow trout,Oncorhynchus mykiss[J]. Aquaculture,2002,204:383-392.

王成辉,李思发,刘志国,等.红鲤生长性状的上位性遗传效应分析[J].中国水产科学,2006,13(4):573-579. Wang Chenghui,Li Sifa,Liu Zhiguo,et al.Genetic analysis of epistatic of growth traits in red common carp[J]. Journal of Fishery Sciences of China,2006,13(4):573-579.

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