太平洋牡蛎中Cg-IL-17和Cg-TGF-β的表达及其免疫作用的研究

董洪亮; 刘乃国; 倪娜; 李彩玉; 郑静; 苗双

doi:10.3969/j.issn.0253-4193.2015.02.011

太平洋牡蛎中Cg-IL-17和Cg-TGF-β的表达及其免疫作用的研究

doi: 10.3969/j.issn.0253-4193.2015.02.011

滨州医学院附属医院临床医学实验室, 山东滨州 256600

基金项目: 山东省高等学校科技计划项目:牡蛎药用基因IL-17的体外表达和组织定位研究(J10LC81).

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出版历程
- 收稿日期: 2014-05-05
- 修回日期: 2014-06-03

Study on the expression and immune function of Cg-IL-17 and Cg-TGF-β in the Pacific oyster,Crassostrea gigas

Clinical Medicine Laboratory, Binzhou Medical University Affiliated Hospital, Binzhou 256600, China

摘要

摘要: 目的:探讨Cg-IL-17和Cg-TGF-β在太平洋牡蛎中表达的组织定位、免疫作用及其可能的机制.方法:采用Real-time PCR、原位杂交和图像分析的方法进行研究.结果:实时定量PCR研究表明,Cg-IL-17和Cg-TGF-β在弧菌感染后的太平洋牡蛎的鳃、外套膜、心包液、唇须、消化道和闭壳肌中表达明显增强.原位杂交实验结果表明,Cg-IL-17和Cg-TGF-β在未染菌组牡蛎的消化道、消化腺、鳃、唇须、外套膜中都有表达;染菌组以上各组织中的Cg-IL-17和Cg-TGF-β表达量均有显著增高,而消化道、消化腺中的表达量明显高于其他组织,闭壳肌中也均有微弱表达;感染弧菌前后两种基因在性腺中均未见表达.相关性分析发现这两种基因表达量在唇须和外套膜中具有明显的相关性.结论:Cg-IL-17和Cg-TGF-β具有多种生物学效应,且都参与了太平洋牡蛎的固有免疫反应;消化道可能是牡蛎主要的免疫反应器官之一;Cg-IL-17和Cg-TGF-β在唇须和外套膜中可能存在调控关系或协同作用.
- 太平洋牡蛎 /
- 牡蛎IL-17 /
- 牡蛎TGF-β /
- 组织定位 /
- 免疫反应
Abstract: Objective:to explore the tissue localization, immune function and the possible mechanism of Cg-IL-17 and Cg-TGF-β in Crassostrea gigas. Methods:using real-time PCR, in situ hybridization and image analysis methods, the expressions of Cg-IL-17 and Cg-TGF-β were studied. Results:the results of real-time PCR showed that the expressions of Cg-IL-17 and Cg-TGF-β were up-regulated in the tissues of C. gigas, including gills, mantle edge, labial palp, digestive tract, adductor muscle after being infected with Vibrio, and so did in pericardiac liquor. The results of in situ hybridization assay indicated that both Cg-IL-17 and Cg-TGF-β expressed in tissues of uninfected group, including digestive tract, digestive glands, gills, labial palp and mantle edge. In Vibrio injected oysters, the expressions of Cg-IL-17 and Cg-TGF-β in above-mentioned tissues increased obviously compared with the same control tissues. The highest expressions of the two genes were found in digestive tract and digestive glands, and the weak expressions were appeared in adductor muscle. But no positive signal was found in gonad of the two groups. Correlation analysis implied that there is significant correlation between Cg-IL-17 and Cg-TGF-β in labial palp and mantle edge. Conclusion:Cg-IL-17 and Cg-TGF-β possess a variety of biological effects. Their expressions markedly increased in C. gigas after Vibrio-infection, suggesting that both cytokines take part in the innate immune response. The digestive tract may be one of the important immune organs of Crassostrea gigas, because the strongest signals of the two genes were found in the tissue. There is regulatory or synergistic action between Cg-IL-17 and Cg-TGF-β in labial palp and mantle edge probably.
- Pacific oyster /
- Cg-IL-17 /
- Cg-TGF-β /
- tissue localization /
- immune response

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