中国沿海9种红藻<i>hsp</i>70基因序列及系统进化分析

杨锐; 王淑刚; 陈娟娟; 徐丽宁; 王亚军; 孙雪

doi:10.3969/j.issn.0253-4193.2013.04.022

中国沿海9种红藻hsp70基因序列及系统进化分析

doi: 10.3969/j.issn.0253-4193.2013.04.022

1.
宁波大学应用海洋生物技术教育部重点实验室, 浙江宁波 315211;宁波大学海洋学院, 浙江宁波 315211
2.
宁波大学科技学院, 浙江宁波 315211

基金项目: 国家自然科学基金(40776077);浙江省自然科学基金(LY12D06003);NMABT项目(2011B81007); ZJAIT(2010R50025-7); ZMBIT(2012R10029-1)。

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出版历程
- 收稿日期: 2012-07-02
- 修回日期: 2012-12-20

Phylogenetic analysis based on hsp70 gene sequences of the nine red seaweeds from Chinese coasts

1.
Ningbo University, Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Zhejiang, Ningbo 315211, China;School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, China
2.
College of Science & Technology Ningbo University, Zhejiang, Ningbo 315211, China

摘要

摘要: 本研究自山东青岛、浙江象山和江苏南通采集共9种红藻样品, 隶属于2纲、5目、6科、8属(据NCBI), 克隆各红藻hsp70 基因, 并对所获序列进行分析。利用特异性引物P1/P3扩增, 得到的目的条带约630 bp, 分析所推导的氨基酸序列发现:所获得片段均位于HSP70的ATPase结构域附近。9种红藻hsp70 序列之间的遗传距离在0.078～0.319之间, 序列相似度在73%～92%之间, 其保守性略低于HSP70蛋白;基因对A或T结尾的密码子表现出很高的偏好性, CGC与TGG这两种密码子在这9种红藻HSP70氨基酸密码子中未出现。上述表明hsp70 及HSP70密码子偏好性可应用于红藻分子系统学研究。基于多物种HSP70构建的进化树可见, Cyanidium caldarium与Cyanidioschyzon merolae strain 10D两种原始红藻的起源早于其他红藻, 紫菜次之, 本研究中9种红藻系统发生符合NCBI的描述。在真菌、藻类和植物中, 营养方式的差异可能是造成HSP70进化树分化的基本原因, 而相同形态类型的物种中, 环境适应是抗逆能力强、遗传结构稳定的物种生物分子进化的重要因素。
- 红藻 /
- hsp70 /
- 系统进化
Abstract: Nine common species of red seaweeds were collected from the coasts of Qingdao Shandong, Nantong Jiangsu and Xiangshan Zhejiang area, which could be classified into 2 classes, 5 orders, 6 families, 8 genus (according to the NCBI). The hsp 70 genes were amplified by PCR from 9 red seaweeds, phylogenetic analysis were carried out based on the hsp70 gene and HSP 70 protein sequences. The obtained target bands amplified by the primers P1/P3 were about 630bp, which were presumed located near the ATPs domain based on the HSPs sequences analysis The distances of hsp70 sequences within 9 red seaweeds were between 0.078 and 0.319, and the similarities of sequences were between 73% and 92%, which conservation were slightly lower than their HSP70 proteins. The codons ended with A and T were preferred in the hsp70 genes of these 9 red seaweeds while the codons of CGC and TGG were not founded. The results of the phylogenetic analysis of hsp70, 18S rDNA and rbcL sequences in other algae, microbes and plants obtained from GenBank suggested that the origin of the primitive red algae Cyanidium caldarium and Cyanidioschyzon merolae strain 10D were more primordially than the other red algae, while the Pyropia species were followed, and the phylogenesis of the 9 species of red seaweeds studied in this paper were coherent with those described in NCBI. The difference of nutrition mode might be the basic factor of HSP 70 differentiation in fungi, algae and plant, while the adaptation to variable environment was the major factor in biological molecular evolution when the organisms were at the same morphological levels, which made the species much stronger in stress resistance and more stable in genetic structures.
- red seaweeds /
- hsp70 /
- phylogeny

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参考文献(27)

Bohuslav Fott. 藻类学[M].上海:上海科学技术出版社, 1981, 2:197．

付万东. 四种海藻热休克蛋白70(HSP70)基因的克隆与表达分析[D].青岛:中国科学院海洋研究院, 2009:25-95.

曾晓起, 胡自民, 段德麟. 核酸分析技术在红藻分子系统学研究中的应用[J].中国海洋大学学报(自然科学版);2005(6):977-983.

Harvey A S, Broadwater S T, Woelkerling W J, et al. Choreonema (Corallinales, Rhodophyta):18S rDNA phylogeny and resurrection of the hapalidiaceae for the subfamilies choreonematoideae, Austrolithoideae, and Melobesioideae[J]. J Phycol, 2003, 39(5):988-998．

万文菊, 于爱莲, 刘含亮等, 溶藻弧菌热休克蛋白70基因克隆基及序列分析[J].中国病原生物学杂志, 2010:498-501.

黄惠芳, 马飞.热激蛋白的分子进化研究[J].厦门大学学报(自然科学版), 2004, 23(增刊):166-170．

Doyle J J, Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phtochemistry Bulletin, 1987, 19:11-15．

Sambrook J, FritschE F, Maniatis T.Molecular Cloning:A Laboratory Manual[M].2nd ed, NewYork:Cold Spring Harbo Laboratory Press, 1989．

Sarah M Richardson, Paul W Nunley, Robert M Yarrington, et al. GeneDesign 3.0 is an updated synthetic biology toolkit[J]. Nucleic Acids Research, 2010.38(8):2603-2606.

Sutherland J E, Lindstrom S C. Nelson W A, et al. A new look at an ancient order: generic revision of the Bangiales (Rhodophyta)[J]. J Phycol, 2011, 47:1131-1151.

Suzanne Fredericq, Max H.Hommersand. Diagnoses and key to the genera of the Gracilariaceae (Gracilariales, Rhodophyta) [J].Biomedical and Life Sciences and Earth and Environmental Science, 1990, 204-205:173-178．

Bernardi G. The human genome: organization and evolution history [J]. Annu Rev Genet, 1995, 29: 445-476．

Thanaraj T A, Argos P. Ribosome-mediated translational pause and protein domain organization[J]. Protein Sci, 1996, 5 (8): 1594-612．

Yu J, Hu S N, Wang J, et al. A draft sequence of the rice genome (Oryzasativa L. ssp. indica) [J]. Science, 2002, 296 (5): 79-92．

石秀凡, 黄京飞, 柳树群, 等.人类基因同义密码子偏好的特征以及与基因GC含量的关系[J].生物化学与生物物理进展, 2002, 29(3):411-414．

Falciatore A, Casotti R, Leblanc C, et al. Transformation of nonselectable reporter genes in marine diatoms[J]. Biotechnology, 1999, 1(2): 239-251．

黄瑞复, 魏蓉城, 晏一祥.生态遗传学[M].北京:科学出版社, 1991．

Mayr E. Happy birthday:80 years of watching the evolutionary scenery[J]. Science, 2004, 305:46-47．

Collén J, Guisle-Marsollier I, Leger J J, et al. Response of the transcriptome of the intertidal red seaweed Chondrus crispus to controlled and natural stresses[J]. New Phytol, 2007, 176: 45-55．

Dahlhoff E P.Biochemical indicators of stress and metabolism: applications for marine ecological studies[J]. Annual Review of Physiology, 2004, 66: 183-207.

Ireland H E, Harding S J, Bonwick G A, et al. Evaluation of heat shock protein 70 as a biomarker of environmental stress in Fucus serratus and Lemna minor [J].Biomarkers, 2004, 9: 139-155．

Smith T R, Tremblay G C, Bradley T M. Hsp70 and a 54 kDa protein (Osp54) are induced in salmon (Salmo salar) in response to hyperosmotic stress[J]. J Exp Zool, 1999, 284: 286-298．

Geraci F, Pinsino A, Turturici G, et al. Nickel, lead, and cadmium induce differential cellular responses in sea urchin embryos by activating the synthesis of different Hsp70s[J]. Biochem Biophys Res Commun, 2004, 322: 873-877．

Piano A, Asirelli C, Caselli F, et al. Hsp70 expression in thermally stressed Ostrea edulis, a commercially important oyster in Europe[J]. Cell Stress Chaperon, 2002, 7: 250-257．

Christine Q, Todd A S, Susan L. Hsp90 as a capacitor of phenotypic variation[J]. Nature, 2002, 417(6889): 598-599．

Renner T, Waters E R. Comparative genomic analysis of the Hsp70s from five diversephotosynthetic eukaryotes[J]. Cell Stress Chaperon, 2007, 12: 172-185.

Lerman D N, Michalak P, Helin A B.Modification of heat-shock gene expression in drosophila melanogaster populations via transposable elements[J].Molecular Biology and Evolution, 2003(20):135-144．

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