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低温胁迫对军曹鱼幼鱼脂代谢相关基因表达的影响

蔡润佳 张静 黄建盛 施钢 潘传豪 谢瑞涛 陈刚 张健东 王忠良 汤保贵

蔡润佳,张静,黄建盛,等. 低温胁迫对军曹鱼幼鱼脂代谢相关基因表达的影响[J]. 海洋学报,2021,43(11):116–122 doi: 10.12284/hyxb2021122
引用本文: 蔡润佳,张静,黄建盛,等. 低温胁迫对军曹鱼幼鱼脂代谢相关基因表达的影响[J]. 海洋学报,2021,43(11):116–122 doi: 10.12284/hyxb2021122
Cai Runjia,Zhang Jing,Huang Jiansheng, et al. Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, Rachycentron canadum[J]. Haiyang Xuebao,2021, 43(11):116–122 doi: 10.12284/hyxb2021122
Citation: Cai Runjia,Zhang Jing,Huang Jiansheng, et al. Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, Rachycentron canadum [J]. Haiyang Xuebao,2021, 43(11):116–122 doi: 10.12284/hyxb2021122

低温胁迫对军曹鱼幼鱼脂代谢相关基因表达的影响

doi: 10.12284/hyxb2021122
基金项目: 南方海洋科学与工程广东省实验室(湛江)项目(ZJW-2019-06);湛江市海洋经济创新发展示范市项目(XM-202008-03);国家海水鱼产业技术体系项目(CARS-47-G08)
详细信息
    作者简介:

    蔡润佳(1995- ),男,广东省汕头市人,研究方向为水产经济动物生物学。E-mail:906636294@qq.com

    通讯作者:

    汤保贵(1975- ),博士,副教授,主要研究方向为鱼类养殖与遗传育种。E-mail:zjtbg@163.com

  • 中图分类号: S965.399

Effects of low temperature stress on the expression of genes related to lipid metabolism of juvenile cobia, Rachycentron canadum

  • 摘要: 将两组军曹鱼(Rachycentron canadum)幼鱼分别在常温(30.5±1.0)℃和低温(20.0±0.5)℃环境下饲养7 d,并于第1天、第4天、第7天3个时间点采集肝脏、肌肉和腹腔脂肪,利用实时荧光定量PCR(qRT-PCR)技术分析5个脂代谢相关基因的表达变化情况,以探究低温胁迫对军曹鱼脂质合成与分解代谢的影响。结果显示,第1天时,肝脏的肉碱脂酰基转移酶-1基因(cpt-1)、脂肪激素敏感脂肪酶基因(hsl)以及肌肉的cpt-1hsl、单酰基甘油酯酶基因(mgl)等显著上调(p<0.05),肝脏、肌肉的乙酰辅酶A羧化酶基因(acc)和脂肪酸合成酶基因(fas)以及腹腔脂肪(IPF)5个脂代谢相关基因表达均显著下调(p<0.05);第4天时,肝脏的cpt-1hslmgl和肌肉的hslmglaccfas以及IPF的cpt-1hslmglacc等表达上调(p<0.05),肝脏的accfas表达显著下调(p<0.05);第7天时,肝脏和IPF的cpt-1hslmglacc和肌肉的hslmglacc等表达上调(p<0.05),肌肉cpt-1和肝脏fas表达显著下调(p<0.05)。结果表明,军曹鱼幼鱼在低温胁迫前期通过抑制脂合成代谢,促进肝脏和肌肉中的脂质水解,通过抑制腹腔脂肪的脂质分解来响应低温胁迫;在低温胁迫后期,军曹鱼幼鱼脂合成和分解代谢均显著提高,且利用脂肪酸提供能量的主要组织由前期的肝脏和肌肉转变为肝脏和腹腔脂肪。
  • 图  1  低温胁迫下军曹鱼幼鱼3个脂分解相关基因表达量

    *表示低温组与同期对照组之间差异显著(p<0.05);**表示差异极显著(p<0.01)

    Fig.  1  Expression of three lipolysis-related genes in juvenile cobia under low temperature stress

    * indicates a significant difference (p<0.05); ** indicates extremely significant difference (p<0.01) compared with the control group in the same period

    图  2  低温胁迫下军曹鱼幼鱼3个脂合成相关基因表达量

    *表示低温组与同期对照组之间差异显著(p<0.05);**表示差异极显著(p<0.01)

    Fig.  2  Expression of three lipid synthesis-related genes in juvenile cobia under low temperature stress

    * indicates a significant difference (p<0.05) ; ** indicates extremely significant difference (p<0.01) compared with the control group in the same period

    表  1  本实验引物序列

    Tab.  1  The primers used in the experiment

    引物名称序列(5′-3′)用途
    acc-FTCGCCAGTCTCCCAACTCCTATacc荧光定量引物
    acc-RACCTGTCCACCTCCTCCTTCATacc荧光定量引物
    fas-FAGCATCCTGTATCGCCCGTTTGAfas荧光定量引物
    fas-RGTCGGTCCTGTGGGTCTCCTTGTfas荧光定量引物
    hsl-FAGCAGTCTGGTTTGGGTTTGGChsl荧光定量引物
    hsl-RAGGTTCTGGGTAATGCGTTCAhsl荧光定量引物
    cpt-1-FTACCGCTTGGCTATGACTGGACcpt-1荧光定量引物
    cpt-1-RTTGCTGGAGATGTGGAAGTTGATGcpt-1荧光定量引物
    mgl-FCACTGCGACCTTTGACCTCTTTGmgl荧光定量引物
    mgl-RAACCATCCTTCTGGGCGTAATCmgl荧光定量引物
    β-actin-FAGGGAAATTGTGCGTGAC内参基因荧光定量引物
    β-actin-RAGGCAGCTCGTAGCTCTT内参基因荧光定量引物
      注:acc为乙酰辅酶A羧化酶基因;fas为脂肪酸合成酶基因;hsl为脂肪激素敏感脂肪酶基因;cpt-1为肉碱脂酰基转移酶-1基因;mgl为单酰基甘油酯酶基因; β-actinβ-肌动蛋白基因。
    下载: 导出CSV
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  • 收稿日期:  2021-01-01
  • 修回日期:  2021-03-30
  • 网络出版日期:  2021-05-08
  • 刊出日期:  2021-12-31

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