15N示踪法测定海洋反硝化速率中29N2浓度的准确定量

曾健; 陈敏; 郑敏芳; 邱雨生

¹⁵N示踪法测定海洋反硝化速率中²⁹N₂浓度的准确定量

1.
厦门大学海洋与地球学院, 福建厦门 361005
2.
厦门大学海洋与地球学院, 福建厦门 361005;近海海洋环境科学国家重点实验室, 福建厦门 361005

基金项目: 国家自然科学基金杰出青年基金项目（41125020）；国际海域资源调查与开发“十二五”课题（DY125-13-E-01）；科技基础性工作专项（2008FY110100）。

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- 收稿日期: 2013-06-25

Accurately quantification of ²⁹N₂ concentration in ¹⁵N tracer derived marine denitrificaiton rates

1.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China
2.
College of Ocean and Earth Sciences, Xiamen University, Xiamen 361005, China;State Key Laboratory of Marine Environment Science, Xiamen University, Xiamen 361005, China

摘要

摘要: 利用¹⁵N示踪法实测南海水体反硝化速率的研究发现，培养水样在长时间密闭放置过程中也会受到外界空气的污染，且其²⁹N₂/²⁸N₂比值恒定为0.007 35。根据空气背景中²⁹N₂/²⁸N₂比值恒定的特征，提出基于质量平衡关系校正空气N₂污染的方法，通过将样品实测²⁹N₂浓度扣除由外界空气贡献的²⁹N₂浓度，可获得由生物反硝化作用所产生的²⁹N₂准确浓度，进而可计算出准确的反硝化速率。经空气²⁹N₂背景校正后，²⁹N₂浓度的偏差明显小于未经校正的结果，且²⁹N₂浓度与培养时间之间的线性相关性显著加强，凸显出空气²⁹N₂背景校正是获取准确反硝化速率的关键。鉴于¹⁵N示踪法已被广泛应用于海洋水体与沉积物反硝化速率的测定中，所提出的空气²⁹N₂背景校正方法具有重要的意义。
- ¹⁵N示踪 /
- 反硝化速率 /
- 空气背景 /
- ²⁹N₂校正
Abstract: ¹⁵N tracer technique was widely applicated in measuring water column denitrification rate in water samples,however,the sealed samples could be contaminated by air during a long time storage. Our study suggested that N₂ in air has a constant ²⁹N₂/²⁸N₂ ratio of 0.007 35. According to this feature,a mass-balance calculation was proposed to calibrate the interference from air background. It helps obtain more accurately ²⁹N₂ concentrations produced by biological activity and further the denitrification rates. After the calibration,the deviations of ²⁹N₂ concentrations were much less than those of the raw data,and the linear correlation between ²⁹N₂ concentration and the incubation time was more significant. It is important to calibrate extra ²⁹N₂ signals from air contamination for accurately quantification of denitrification rate,considering the ¹⁵N technique has been widely used in pelagic and beneath denitrification rate measurements.
- ¹⁵N tracer /
- denitrification rate /
- air contamination /
- ²⁹N₂ calibration

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参考文献(43)

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¹⁵N示踪法测定海洋反硝化速率中²⁹N₂浓度的准确定量

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Accurately quantification of ²⁹N₂ concentration in ¹⁵N tracer derived marine denitrificaiton rates

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