中国对虾微卫星四重PCR技术的建立及其在模拟放流效果评估方面的应用

李伟亚; 王伟继; 孔杰; 金显仕; 阮晓红; 罗坤

中国对虾微卫星四重PCR技术的建立及其在模拟放流效果评估方面的应用

1.
中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室, 山东青岛 266071;上海海洋大学水产与生命学院, 上海 201306
2.
中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室, 山东青岛 266071

基金项目: 国家自然科学基金面上项目(41076109);农业部公益性行业专项(200903005)。

计量
- 文章访问数: 1218
- HTML全文浏览量: 4
- PDF下载量: 1276
- 被引次数: 0
出版历程
- 收稿日期: 2012-02-16
- 修回日期: 2012-06-05

Establishment of the microsatellite quadruple PCR technology for Fenneropenaeus Chinensis and its application in the evaluation on the effect of releasing simulation

1.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China
2.
Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

摘要

- 中国对虾 /
- 微卫星 /
- 多重PCR /
- 家系识别
- Fenneropenaeus chinensis /
- microsatellite /
- multi-PCR /
- pedigree tracing

HTML全文

参考文献(24)

GODIN D M,CARR W H,HAGINO G,et al.Evaluation of a fluorescent elastomer internal tag in juvenile and adult shrimp Penaeus vannamei[J].Aquaculture,1996,139:243-248.

MEEHAN D,XU Z,ZUNIGA G,et al.High frequency and large number of polymorphic microsatellites in cultured shrimp,Penaeus (Litopenaeus) vannamei(Crustacea:Decapoda)[J].Marine Biotechnology,2003,5:311-330.

徐炳庆.山东近海中国对虾增殖放流的研究.上海:上海海洋大学,2011.

HULAK M,KASPAR V,KOHLMANN K,et al.Microsatellite-based genetic diversity and differentiation of foreign common carp (Cyprinus carpio) strains farmed in The Czech Republic [J].Aquaculture,2010,298:194-201.

BORRELL Y J,ALVAREZ J,BLANCO G,et al.A parentage study using microsatellite loci in a pilot project for aquaculture of the European anchovy Engraulis encrasicolus L.[J].Aquaculture,2011,310:305-311.

ARABNEZHAD H,BAHAR M,POUR A T.Evaluation of genetic relationships among Iranian pistachios using microsatellite markers developed from Pistacia khinjuk Stocks[J].Scientia Horticulturae,2011,128:249-254.

RUAN X H,WANG W J,KONG J,et al.Genetic linkage mapping of turbot (Scophthalmus maximus L.) using microsatellite markers and its application in QTL analysis[J].Aquaculture,2010,308:89-100.

SEKINO M,SAITOH K,YAMADA T,et al.Microsatellite-based pedigree tracing in a Japanese flounder Paralichthys olivaceus hatchery strain:implications for hatchery management related to stock enhancement program [J].Aquaculture,2003,221:255-263.

SEKINO M,SAITOH K,YAMADA T,et al.Genetic tagging of released Japanese flounder (Paraalichthys olivaceus) based on polymorphic DNA markers[J].Aquaculture,2005,244:49-61.

DONG S R,KONG J,ZHANG T S,et al.Parentage determination of Chinese shrimp (Fenneropenaeus chinensis) based on microsatellite DNA markers[J].Aquaculture,2006,258:283-288.

董世瑞,孔杰,张庆文,等.微卫星分型方法进行中国明对虾家系系谱鉴定[J].海洋学报,2006,28(3):1-5.

王鸿霞,吴长功,张留所,等.微卫星标记应用于凡纳滨对虾家系鉴别的研究[J].遗传,2006,28(2):179-183.

王霞.微卫星分型技术用于凡纳滨对虾遗传多样性和亲缘关系分析.杨凌:西北农林科技大学,2009.

MOCHIZUKI H,NAKAMURA K,SATO H,et al.Multiplex PCR and Genescan analysis to detect immunoglobulin heavy chain gene rearrangement in feline B-cell neoplasms[J].Veterinary Immunology and Immunopathology,2011,143:38-45.

CINDY L,JONES V.Fluorescence PCR and GeneScan analysis for the detection of CAG repeat expansions associated with huntington's disease[J].Methods in Molecular Biology,2003,217(2):101-108.

孔杰,高焕,于飞,等.微卫星三重PCR基因扫描技术在中国明对虾家系标识中的应用[J].中国水产科学,2007,14(1):59-66.

罗坤,张天时,孔杰.定向交尾技术在中国对虾家系构建中的初步研究[J].海洋水产研究,2007,28(1):71-75.

FITZGERALD J L,SHEEHAN T F,KOCIK J F.Visibility of visual implant elastomer tags in Atlantic salmon reared for two years in marine net-pens[J].North American Journal of Fisheries Management,2004,24:222-227.

SIMON J.Evaluation of marking European silver eels with visible implant elastomer tags and alcian blue[J].Journal of Fish Biology,2007,70(1):303-309.

王伟继.Ⅰ.中国对虾(Fenneropenaeus chinensis)AFLP分子标记遗传连锁图谱的构建以及相关性状的QTL定位研究; Ⅱ.蓝鳃太阳鱼(Lepomis macrochirus)AFLP分子标记遗传连锁图谱的构建及性别决定机制初探.青岛:中国海洋大学,2008.

KALINOWSKI S T,TAPER M L,MARSHALL T C.Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment[J].Molecular Ecology,2007,16:1099-1006.

刘晓鑫,谢传晓,赵琦,等.基于SSR荧光标记技术的玉米群体混合样本基因频率分析方法[J].中国农业科学,2008,41(12):3991-3998.

黎中宝,APPLEYARD S A,ELLIOTT N G.多元PCR在黑鲍(Haliotis rubra)微卫星遗传研究中的应用[J].海洋与湖沼,2005,36(4):319-325.

孙昭宁,刘萍,李健,等.微卫星 DNA标记用于中国对虾家系亲子关系鉴定[J].海洋水产研究,2007,28(3):8-14.

施引文献

资源附件(0)

访问统计