厚蟹线粒体16SrRNA基因序列分析及系统发育研究
Molecular phylogeny of the crab genera Helice and Helicana based on the partial sequences of mitochondrial 16S rRNA gene
-
摘要: 对我国沿海天津厚蟹6个群体、侧足厚蟹4个群体、伍氏仿厚蟹2个群体和日本仿厚蟹1个群体的线粒体16S rRNA基因片段进行了序列测定;结合从GenBank下载的其他厚蟹序列,分析了厚蟹分子系统发育关系。除天津厚蟹丹东群体的2个个体16S rRNA基因片段长度为526 bp外,其他天津厚蟹和侧足厚蟹均为525 bp;除日照群体和丹东群体外,其他每个群体的个体之间没有序列差异,天津厚蟹和侧足厚蟹共有5个单倍型。伍氏仿厚蟹16S rRNA基因片段长度均为525 bp,群体、个体之间均无序列差异。日本仿厚蟹16S rRNA基因片段长度均为523 bp,个体之间无序列差异。其A,T,G, C含量只有略微的差异,A+T含量(72.3%~73.7%)明显高于G+C含量。4种厚蟹所有序列比对,在526 bp序列上共有变异位点36处和4处插入/缺失,其中简约信息位点26处,转换/颠换的平均值为2.8。在5个单倍型中,侧足厚蟹泉州、宁波群体的6个个体与天津厚蟹宁波、日照群体的4个个体的序列都相同,共享1个单倍型;而且5个单倍型之间的遗传距离很小,仅为0.19%~1.15%,表明二者为同一物种的不同形态类型。采用NJ法, ML法和MP法构建的厚蟹/张口蟹复合群系统进化树的拓扑结构基本一致。结果显示,天津厚蟹和侧足厚蟹的10个群体的5个单倍型和台湾厚蟹首先聚到一起,形成侧足厚蟹复合体后,再与三齿厚蟹聚为一支;日本仿厚蟹与德氏仿厚蟹先聚在一起,然后再与伍氏仿厚蟹聚为一支;均得到99%置信度的支持,表明厚蟹属蟹类和仿厚蟹属蟹类均为单系,支持将厚蟹亚属和仿厚蟹亚属分别提升为属。分子数据及系统树的拓扑结构亦支持将假厚蟹亚属和拟厚蟹亚属分别提升为属,以及形态学上将厚蟹/张口蟹复合群分为7个属的结论。Abstract: The partial sequences of mitochondrial 16S rRNA gene of Helice tientsinensis (six populations), H. latimera (four populations), Helicana wuana (two populations) and H. japonica(one population) from the coast of China were determined and subjected to phylogenetic analysis with the inclusion of other species of Helice and Helicana obtained from the GenBank. The length of sequence of Helice tientsinensis and H. latimera was 525 bp, excluding 526 bp of two individuals from Dandong population of H. tientsinensis. Five haplotypes were found in them. The lengths of sequence of Helicana wuana and H. japonica were 525bp and 523bp respectively. There were no differences of sequence among individuals within populations of them, except Rizhao and Dandong populations. The A, T, G and C contents of them were similar, and the AT content(72.3%~73.7%)was higher than GC content. The resultant sequences of H. tientsinensis, H. latimera, H. wuana and H. japonica were 526bp long with 4 indels, of which 36 sites were variable and 26 sites were parsimoniously informative, and average transition/transversion ratio was 2.8. Among the five haplotypes, six individuals of H. latimera from Quanzhou and Ningbo populations and four individuals of H. tientsinensis from Ningbo and Rizhao populations shared one haplotype, and genetic distances among them were very small(0.19%~1.15%), implying that they may represent different morphotypes of the same species. Molecular phylogenetic trees indicated that the genera Helice and Helicana were monophyletic respectively, the large net genetic distances between them supported that they were updated from subgenus to genus. The molecular data also supported that the Pseudohelice and Parahelice were updated from subgenus to genus, and Helice/Chasmagnathus complex was divided into seven genera by morphology.
-
Key words:
- Helice /
- Helicana /
- mitochondrial DNA /
- 16S ribosomal RNA gene /
- phylogeny
-
DAI Ai-yun, YANG Si-liang. Crabs of the China Seas[M]. Beijing: China Ocean Press, 1991: 15,551-555. SCHUBART C D,CUESTA J A,FELDER D L. Glyptograpsidae, a new brachyuran family from Central America: larval and adult morphology and a molecular phylogeny of the Grapsoidea[J]. Journal of Crustacean Biology, 2002, 22(1): 28-44. PARISI B. I decapodi giapponesi del Museo di Milano: Ⅵ. Catometopa e Paguridea[J].Atti della Società Italiana di Scienze Naturali, 1918,56(314): 90-115. RATHBUN M J. New and rare Chinese crabs[J]. Lingnan Science Journal, 1931(dated 1929), 8:75-125. SAKAI K, YATSUZUKA K. Notes on some Japanese and Chinese Helice with Helice (Helicana) n. subgen., including Helice (Helicana) japonica n. sp. (Crustacea: Decapoda)[J]. Senckenbergiana Biology, 1980,60(5/6): 393-411. SAKAI K, TüRKAY M, YANG Si-liang. Revision of the Helice/Chasmagnathus complex (Crustacea: Decapoda:Brachyura)[J]. Abhandlungen der Senckenbergischen Naturforschenden Gesellschaft,2006,565:1-76. NG P K L, GUINOT D, DAVIE P J F. Systema brachyurorum:partⅠ. An annotated checklist of extant brachyuran crabs of the world [J]. The Raffles Bulletin of Zoology (Supplement), 2008,17:1-286. 孙红英,周开亚,景开颜, 等. 从线粒体16S rDNA部分序列探讨厚蟹属的系统学位置[J]. 南京师大学报:自然科学版,2002, 25(1):15-19. KITAURA J, WADA K,NISHIDA M. Molecular phylogeny of grapsoid and ocypodoid crabs with special reference to the genera Metaplax and Macrophthalmus[J]. Journal of Crustacean Biology, 2002, 22(3): 682-693. SCHUBART C D,CANNICCI S,VANNINI M,et al. Molecular phylogeny of grapsoid crabs (Decapoda, Brachyura) and allies based on two mitochondrial genes and a proposal for refraining from current superfamily classification[J]. Journal of Zoological Systematics and Evolutionary Research, 2006, 44 (3):193-199. SHIH H T, SUZUKI H. Taxonomy, phylogeny, and biogeography of the endemic mudflat crab Helice/Chasmagnathus complex (Crustacea: Brachyura: Varunidae) from East Asia[J]. Zoological Studies, 2008,47(1): 114-125. BOUCHON D, SOUTY-GROSSET C, RAIMOND R. Mitochondrial DNA variation and markers of species identity in two penaeid shrimp species: Penaeus monodnn Fabricius and P. japonicus Bate[J]. Aquiculture,1994,127(2-3):131-144. TAMURA K, DUDLEY J, NEI M, et al. MEGA4: molecular evolutionary genetics analysis (MEGA) software, vers.4.0[J]. Molecular Biology and Evolution,2007,24:1596-1599. FELESENSTEIN J. Confidence limits on phylogenies: An approach using the bootstrap[J]. Evolution,1985,39(4):783-791. 徐敬明,张俊丽,方华华,等. 相手蟹属两种蟹类线粒体16S rRNA基因序列的比较[J]. 水产科学,2006,25(9): 443-447. SHIH H T, NG P K L, CHANG H W. Systematics of the genus Geothelphusa (Crustacea: Decapoda, Brachyura, Potamidae) from southern Taiwan: a molecular appraisal[J]. Zoological Studies,2004,43(3): 561-570. SOTELO G, MORáN P, POSADA D. Genetic identification of the northeastern Atlantic spiny spider crab as Maja brachydactyla Balss, 1922[J]. Journal of Crustacean Biology, 2008, 28(1):76-81. 徐敬明,杨帆,孙世春. 厚蟹属种群形态量度分析[J]. 水生生物学报,2010,34(3):533-540. YEO D C J, SHIH H T, MEIER R, et al. Phylogeny and biogeography of the freshwater crab genus Johora(Crustacea: Brachyura: Potamidae) from the Malay Peninsula, and the origins of its insular fauna[J]. Zoologica Scripta,2007,36(3):255-269. SHIH H T, NG P K L, SCHUBART C D, et al. Phylogeny and phylogeography of the genus Geothelphusa(Crustacea: Decapoda, Brachyura, Potamidae) in southwestern Taiwan based on two mitochondrial genes[J]. Zoological Science,2007,24(1):57-66. SCHUBART C D, KOLLER P. Genetic diversity of freshwater crabs(Brachyura:Sesaimidae) from central Jamaica with description of a new species[J]. Journal of Natural History, 2005,39(6):469-481. SCHUBART C D,CUESTA J A, RODRíGUEZ A. Molecular phylogeny of the crab genus Brachynotus (Brachyura:Varunidae)based on the 16S rRNA gene[J]. Hydrobiologia, 2001, 449(1-3):41-46. FAN Yu, LI Xin-zheng, SONG Lin-sheng, et al. Phylogenetic relationships of five species of Dorippinae (Crustacea,Decapoda) revealed by 16S rDNA sequence analysis[J]. Acta Oceanologica Sinica, 2004,23(3):513-519.
点击查看大图
计量
- 文章访问数: 2005
- HTML全文浏览量: 25
- PDF下载量: 1191
- 被引次数: 0