致病性副溶血弧菌生物膜形成特性研究

陈珍; 覃映雪; 邹文政; 徐晓津; 邢颜丽; 鄢庆枇

致病性副溶血弧菌生物膜形成特性研究

集美大学水产学院福建省高校水产科技与食品安全重点实验室, 福建厦门 361021

基金项目: 国家"八六三"计划项目(2007AA09Z115);厦门市科技项目(3502Z20073019;3502Z20073020)。

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出版历程
- 收稿日期: 2010-01-17

Characteristics of biofilm formation of pathogenic Vibrio parahaemolyticus

Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China

摘要

摘要: 采用改良的微孔板法测定了12株弧菌的形成生物膜的效果,选择形成生物膜效果最好的副溶血弧菌ND-02,进一步研究了环境因子对其生物膜形成的影响。实验结果显示副溶血弧菌ND-02菌株在静置培养24～36 h后形成成熟的生物膜,在起始菌浓度为10⁷～10⁸CFU/mL形成生物膜的量最大;在30 ℃,NaCl浓度为3%～ 5%,pH偏弱碱性时的生物膜OD₅₉₀值最大;Ca²⁺ 促进副溶血弧菌生物膜的形成,而Mg²⁺ 抑制生物膜的形成;副溶血弧菌在分别经大黄鱼表皮黏液、肠黏液和肝脏提取液包被的的基质上形成生物膜的作用明显,鳃黏液和脾脏提取液中次之,肌肉提取液包被后生物膜形成量最低。以上结果表明,副溶血弧菌ND-02菌株能形成稳定而明显的生物膜,而且生物膜的形成受温度、NaCl浓度、pH值等环境因子的影响。
- 副溶血弧菌 /
- 生物膜 /
- 环境因子
Abstract: Biofilm formation of 12 strains of Vibrio were determinated using the method of modified microtiter-plate test,and V. parahaemolyticus strain ND-02 was chosen for the further study on the influence of different environmental factors on bacterial biofilm formation .The results show that V. parahaemolyticus strain ND-02 forms mature biofilm in 24～36 h,V. parahaemolyticus with original bacterial concentrations of 10⁷ and 10⁸CFU/mL shows the largest biofilm quantity; the OD₅₉₀ value of bacterial biofilm increases with the incubation temperature from 10 ℃ to 30 ℃ and peaks at 30 ℃;the OD₅₉₀ value of bacterial biofilm fluctuates with different NaCl concentrations optimized at 3% to 5%,and OD₅₉₀ values are remarkably higher at pH 7～10,and peak at pH 7.Addition of Ca²⁺ obviously promoted the bacterial biofilm formation while Mg²⁺ played as an opposite role. V. parahaemolyticus strain ND-02 showed better biofilm formation on the wells coated with liver extract,skin mucus and intestinal mucus of Pseudosciaena crocea,the less biofilm was found on the wells coated with muscle extract. The results indicate that V. parahaemolyticus strain ND-02 develops stable and evident biofilm,the biofilm formation is remarkably affected by environmental factors, such as temperature, pH, NaCl concentration,Ca²⁺ ,Mg²⁺ . The results also indicate that the bacterial biofilm formation of V. parahaemolyticus is governable,which will be useful for the epidemic control in fish culture.
- Vibrio parahaemolyticus /
- biofilm /
- environmental factors

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