黄海西北近岸沉积物中细菌群落空间分布特征

张健; 赵阳国; 李海艳; 白洁; 田伟君

黄海西北近岸沉积物中细菌群落空间分布特征

中国海洋大学海洋环境与生态教育部重点实验室,山东青岛 266100

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出版历程
- 收稿日期: 2009-07-05
- 修回日期: 2010-02-02

Temporal and spatial distribution characterization of bacterial community in sediments from the inshore of the northwest Huanghai Sea

Key Laboratory of Ministry of Education for Marine Environment and Ecology, Ocean University of China, Qingdao 266100, China

摘要

摘要: 采用16S rDNA文库和变性梯度凝胶电泳(DGGE)技术,对黄海西北部3个近岸站位沉积物中细菌群落多样性及空间分布特征进行了调查和解析。对表层沉积物16S rDNA序列统计表明,各站位细菌群落多样性很高,γ-和δ-变形菌纲分别占克隆序列总数的20%~32%,是沉积物中的绝对优势类群。DGGE图谱分析表明,同一站位中不同深度的细菌群落结构相似性较高,而不同站位间群落结构相差较远。研究表明在黄海西北近岸沉积物中细菌群落多样性较高,优势类群明显,在较小尺度范围内群落结构的垂直变化不明显。
- 16SrDNA文库 /
- 变性梯度凝胶电泳 /
- 细菌群落
Abstract: 16S rDNA clone library combining with denaturing gradient gel electrophoresis (DGGE) technique was used to reveal the bacterial community diversity and the spatial distribution characteristics from the three stations of the sediment of the northwest Huanghai Sea. The bacterial 16S rDNA libraries of the surface sediments showed that at all three stations composed high diversity of bacterial communities, γ-and δ-proteobacteria were the donminant groups, respectively, accounting for 20% and 32% of the total sequences. DGGE profiles showed that the bacterial community structure presented a high degree of similarity at the same stations at different depths, but demonstrated far distance from different stations. The present study shows that the bacteria from the coastal sediments have a high community diversity and possess dominant groups in bacterial community, but on a smaller scale, the vertical changes of community structure are not obvious.
- 16S rDNA clone library /
- denaturing gradient gel electrophoresis (DGGE) /
- bacterial community

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