笛鲷鱼类的线粒体DNA控制区结构及其系统发育分析
Structure of the mitochondrial DNA control region of snapper species and their phylogenetic relationship
-
摘要: 采用PCR扩增直接测序的方法获得了11种笛鲷鱼类的线粒体DNA控制区全序列。结合从GenBank中下载的6种笛鲷鱼类的相应序列进行结构分析,可以将笛鲷鱼类的控制区分为终止序列区、中央保守区和保守序列区三个区域,找到了终止相关的序列ETAS以及一系列保守序列(CSB-F,CSB-E,CSB-D和CSB-1,CSB-2,CSB-3),并给出了它们的一般形式。以黄谐鱼为外群,采用NJ法和ME法构建笛鲷鱼类的分子系统树。分子系统发育分析表明,线粒体DNA控制区序列适合于笛鲷鱼类的系统发育分析,而且支持Allen关于笛鲷属的系统形态分类学观点。Abstract: Complete sequences of the mitochondrial DNA control region from 11 species of snapper species were amplified by polymerase chain reaction (PCR) and directly sequenced. The structure of the control region was analyzed with six other species of snapper from GenBank. According to the alignment, three domains, the termination associated sequence domain (TAS), the central conserved domain (CD) and the conserved sequence block domain (CSB), were identified in the mtDNA control region of snapper species. The extended termination associated sequence (ETAS), three conserved blocks (CSB-F, CSB-D,CSB-D) in the central conserved sequence block domain and three conserved sequence blocks (CSB1,CSB2,CSB3) in the conserved sequence block domain were successfully identified. With Emmelichthys struhsakeri as outgroup, the phylogenetic trees were constructed using the neighbor-joining (NJ) method and the minimum evolution (ME) method. It can be concluded that the control region can be used for the phylogenetic analysis, and the phylogenetic relationships from mtDNA data are consistent with Allen’s morphology-based system.
-
Key words:
- snapper species /
- mitochondrial DNA /
- control region /
- phylogeny
-
肖武汉,张亚平.鱼类线粒体DNA的遗传与进化[J]. 水生生物学报, 2000, 24(4): 384—391. 唐琼英,杨秀平,刘焕章. 刺鲃基于线粒体细胞色素b基因的生物地理学过程[J].水生生物学报, 2003, 27(4): 352—365. 朱世华,杨迎春,沈锡权, 等. 从细胞色素b基因序列探讨笛鲷属的分子系统发生关系[J]. 动物学报, 2006, 52(3): 514—521. 李青青,张亚平. 基于线粒体控制区序列的猕猴属系统发育研究[J]. 动物学研究, 2004, 25(5): 385—390. 陶峰勇,王小明,郑合勋,等. 中国大鲵四种群的遗传结构和地理分化[J]. 动物学研究, 2005, 26 (2): 162—167. 成庆泰,郑葆珊. 中国鱼类系统检索 . 北京: 科学出版社, 1987. 周发林,江世贵,苏天凤,等.6种笛鲷属鱼类Cyt b基因片段序列的比较[J].热带海洋学报,23(4):87—92. 周发林,江世贵,苏天凤,等. 6种笛鲷属鱼类线粒体16S rRNA基因片段的序列比较[J].中国水产科学,11(2):99—103. 张俊彬,黄良民,陈真然. AFLP技术在笛鲷的仔鱼鉴定及其分类学上的研究[J]. 海洋学报, 2005, 27(2): 165—171. 张俊彬,刘昕.基因组AFLP 分析及线粒体12S rRNA 序列变异在笛鲷科系统发育上的比较[J].科学通报, 2006,51:120—124. GUO Y S, WANG Z D, LIU C W, et al. Phylogenetic relationships of South China Sea snappers (Genus Lutjanus; Family Lutjanidae) based on mitochondrial DNA sequences[J]. Mar Biotechnol (NY), 2007,9(6): 682—688. 谭围,王中铎,郭昱嵩,等. 孟加拉笛鲷线粒体基因组序列结构及其进化[J]. 中国生物化学与分子生物学报,2009, 25(3):287—291. MILLER T L, CRIBB T H. Phylogenetic relationships of some common Indo-Pacific snappers (Perciformes : Lutjanidae) based on mitochondrial DNA sequences, with comments on the taxonomic position of the Caesioninae[J].Molecular Phylogenetics and Evolution,2007,44(1): 450—460. 卢圣栋. 现代分子生物学实验技术[M],第2版.北京:中国协和医科大学出版社, 1999. LEE W J, CONROY J, HOWELL W H, et al. Structure and evolution of teleost mitoehondrial control regions[J]. Joumal of Molecular Evolution[J].1995,41(1):54—66. 朱世华,郑文娟,邹记兴, 等. 鲹科鱼类线粒体DNA控制区及系统发育关系[J].动物学研究, 2007,28(6): 606—614. 张燕,张鹗,何舜平. 中国鲿科鱼类线粒体DNA控制区结构及其系统发育分析[J]. 水生生物学报, 2003, 27(5): 463—467. 唐琼英,刘焕章,杨秀平, 等. 沙鳅亚科鱼类线粒体DNA控制区结构分析及系统发育关系的研究[J]. 水生生物学报,2005, 29(6):645—653. 刘焕章. 鱼类线粒体DNA控制区的结构和进化:以鳑鮍鱼类为例[J].自然科学进展,2002, 3(12):266—270. ZHAO J L, WANG W W, LI S F, et al.Structure of the mitochondrial DNA control region of the sinipercine fishes and their phylogenetic relationship[J]. Acta Genetica Sinica, 2006, 33 (9):793—799. WOLSTENHOLME D R. Animal mitochondrial DNA: structure and evolution[M]//Mitochondrial Genomes. San Diego:Academic press. 1992:173—372. SBISA E, TANZARIELLO F, REYES A, et al. Mammalian mitochondrial D-loop region structural analysis: identification of new conserved sequences and their functional and evolutionary implications[J]. Gene, 1997, 205: 125—140. SACCONE C, PESOLE G, SBISA E. The main regulatory region of mammalian mitochondrial DNA: structure-function model and evolutionary pattern[J]. J Mol Evol, 1991, 33: 83—91. CHEN I S, HSU C H, HUI C F, et al. Sequence length and variation in the mitochondrial control region of two freshwater gobiid fishes belonging to Rhinogobius (Teleostei:Gobioidei) [J]. Journal of Fish Biology,1998,53: 179—191. MATTHEW A S, SCOTT V E. Dynamics and phylogenetic implications of mtDNA control region sequences in new world jays(Aves:Corvidae) [J].J Mol Evol, 2000,51:97—109. LOCKHART P J, PENNY D, MEYER A. Testing the phylogeny of swordtail fishes using decomposition and spectral analysis[J]. Mol Evol.1995, 41(5):666—74. ALLEN G R. Snappers of the world: an annotated and illustrated catalogue of Lutjanidae species known to date[M]// FAO Fish Synop.Rome:FAO. 1985. ZHANG J B, HUANG L M, HUO H Q. Larval identification of Lutjanus Bloch in Nansha coral reesfs by AFLP molecular method[J]. J Exp Mar Biol Ecol, 2004, 298: 3—20. YAAKUB S M, BELLWOOD D R, HERWERDEN L, et al. Hybridization in coral reef fishes: introgression and bi-directional gene exchange in thalassoma (family Labridae)[J]. Mol Phylogenet Evol, 2006,40(1):84—100.
点击查看大图
计量
- 文章访问数: 1209
- HTML全文浏览量: 15
- PDF下载量: 908
- 被引次数: 0