中国沿海六个地理群体短蛸的遗传变异研究

吕振明; 李焕; 吴常文; 樊甄姣; 张建设

中国沿海六个地理群体短蛸的遗传变异研究

浙江海洋学院浙江省海洋养殖装备与工程技术重点实验室,浙江舟山 316000

基金项目: 国家科技支撑计划项目(2007BAD43B00);浙江省高等学校优秀青年教师资助计划项目。

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- 收稿日期: 2009-04-08

Genetic variation of Octopus ocellatus populations in Chinas coastal waters based on the COI gene analysis

Zhejiang Provincial Key Laboratory of Marine Culture Equipement and Technology, Zhejiang Ocean University, Zhoushan 316000,China

摘要

摘要: 采用线粒体COI基因测序技术分析了我国沿海重要经济头足类短蛸(Octopus ocellatus)6个地理群体的遗传结构及其变异,结果表明我国短蛸资源的遗传多样性相对丰富,在654 bp长度的核苷酸片段中共检测到42个多态位点,多态位点比例达6.42 %,检测的60个个体中共获23个单倍型,单倍型多样性指数为0.200~0.867,平均核苷酸多样性指数为0.000 3~0.009 7,平均核苷酸差异数为0.200 ~ 6.311,高于多数海洋头足类的遗传变异。对6个群体的遗传结构进行检测表明,我国的短蛸群体间除部分群体外均存在显著的遗传分化(P＜0.05),遗传结构基本符合脚踏石模型。聚类分析表明6群体已明显分化为两个类群,一个由大连、青岛、连云港群体组成,另一个由舟山、上海和广州群体组成,两类群间存在20个固定位点核苷酸差异,编码的蛋白质存在2个固定氨基酸残基的差异。两类群的分化系数达0.860 2(P＜0.01),基因流大大小于1;AMOVA检测显示仅有12.72%的遗传差异存在于群体内部,而87.28%的遗传差异存在于群体间。短蛸遗传变异的研究成果可为今后我国短蛸资源更好的开发和管理提供理论基础。
- 地理群体 /
- 短蛸 /
- 遗传变异
Abstract: Studies on genetic variation in marine organisms are an important component of successful and sustainable management of marine fishery resources.Genetic variation of six Octopus ocellatus populations in Chinas coastal waters were investigated using the mitochondrial gene(COI) sequencing method. Results show that ,compared with other cephalopod species, O.ocellatus shows higher genetic diversity in populations. 42 polymorphic sites were detected in 654 bp aligned sequence, which reprented for 6.42% of the total length of sequenced COI gene. 23 hapotypes were detected out of 60 individuals from six populations. The hapotype diversity, mean nucleotide diversity and average number of nucleotide differences reached 0.200~0.867, 0.000 3~0.009 7, 0.200~6.311, respectively. Molecular variance analysis revealed strong genetc sturcture in six O.ocellatus populations(P＜0.05). A pattern of genetic-isolation-by-distance, comforming to a stepping stone model, was detected when plotting gene flow against geographic distance.UPGMA tree constructed shows that six O.ocellatus populations studied can be divided into two lineages. One is consisited of Dalian, Qingdao,Lianyungang plulations. The other is consisted of other three populations. Between them, 20 fixed nucleotide sites and two fixed amino acid sites subtitute were detected along the gene sequence and coded protein, respectively. The Fst between them reached 0.8602 (P＜0.01) and the gene flow between them turned out to be much lower than 1. AMOVA test, which showing 12.72% of the genetic variation lies within populations and 87.28% lies between populations, further suggested the genetic differentiation between these two lineages. These findings in the aspect of genetic variation of O.ocellatus will contribute to better exploitment and refined management of this precious cephalopod resources along the coast of China in future.
- geographycal population /
- Octopus ocellatus /
- genetic variation

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