斜带髭鲷后肠、头肾和脾脏中肥大细胞超微结构观察
Ultrastructural observations on the mast cells in hindgut, head kidney and spleen of Hapalogenys nitens
-
摘要: 应用电镜技术研究了斜带髭鲷的后肠、头肾及脾脏中肥大细胞的超微结构。结果显示:在所研究的器官组织中均发现有肥大细胞,但以后肠和头肾分布较多;这些肥大细胞的表面具有胞质突起或表面折叠。根据其特征性胞浆颗粒所含特殊亚结构的不同,可初步认为斜带髭鲷肥大细胞可能存在Ⅰ型和Ⅱ型两种亚型。Ⅰ型肥大细胞主要分布于后肠粘膜固有层、粘膜下层和头肾的结缔组织中,其特征性胞浆颗粒具有微粒样特殊亚结构;Ⅱ型肥大细胞仅分布于头肾和脾脏的结缔组织中,其特征性胞浆颗粒则具有漩涡样、线团样、蜂巢样、细粒状、无定形和混合型等多种晶格状特殊亚结构。同时,这两种亚型肥大细胞均存在脱颗粒现象。由观察结果可知,斜带髭鲷后肠、头肾及脾脏中存在大量的肥大细胞,并且其特征性胞浆颗粒具有精细的特殊亚结构。Abstract: The mast cells in hindgut, head kidney and spleen of H apalogenys nitens were investigated by using transmission electron micro scopy.The results showed that mast cells possessing cytoplasmic processes or surface folds were found in all the organs studied, but were especially abundant in hindgut and head kidney.The mast cells perhaps could be classified into two major subtypes by the special substructures of their characteristic cytoplasmic granules and referred to as type Ⅰ and type Ⅱ mast cells.The cytoplasmic granules with tiny particulate substructure were characteristic of the type Ⅰ mast cells, which distributed mainly in the lamina propria and submucosa layers of the hindgut and in the connective tissues of the head kidney.And the type Ⅱ mast cells locating only in the connective tissues of the head kidney and spleen were characterized by their cytoplasmic granules exhibiting a variety of crystalline substructural patterns, such as whorl, thread, honeycomb, particulate, amorphism, and mixture of these substructural patterns.The degranulation was also found in the two subtypes of mast cells.Our results demonst rate that numerous mast cells containing characteristic cytoplasmic granules with delicate special substructures are present in hindgut, head kidney and spleen of H apalogenys nitens.
-
Key words:
- mast cell /
- cytoplasmic granule /
- ultrast ructure /
- special substructure /
- H apalogenys nitens
-
许乐仁,江萍,高登慧,等.硬骨鱼肥大细胞的研究进展[J].中国水产科学,2003,10(5):425-430. GALLI S L.Biology of disease.New insight into"The riddle of the mast cells":Microenvironmental regulation of mast cell development and phenotypic heterogeneity[J].Lab Invest,1990,62(1):5-33. MICHELS N A.The mast cell in the lower vertebrates[J].Cellule,1923,33:338-462. 杨筱珍,高登慧,许乐仁.胡子鲇肥大细胞的组织化学及形态学[J].中国水产科学,2003,10(2):106-109. 许乐仁,杨筱珍,高登慧.草鱼淋巴器官中的肥大细胞[J].水产学报,2003,27(3):233-237. ROCHA J S,CHIARINI-GARCIA H.Mast cell heterogeneity between two different species of Hoplias sp.(Characifornles:Erythrinidae):Response to fixatives,anatomical distributiont histochemical contents aM ultrastructural features[J].Fish & Shellfish Immunology,2007,22:218-229. CRAIG S S,SCHECHTER N M,SCHWARTZ L B,Ultrastructural analysis of human T and TC mast cells identified by immunoelectron microscopy[J].Lab Invest,1988,58(6):682-691. CRAIG S S,SCHECHTER N M,SCHWARTZ L Ultrastructural analysis of maturing human T and TC mast cells in situ[J].Laboratory Investigation,1989,60(1):147-157. XU LR,CARRMM,BLAND A P,et al.Histochemistry and morphology of porcine mast cells[J].Histochem J,1993,25(7):516-522. REITE O B,EVENSEN φ.Mast cells in the swimbladder of Atlantic salmon,Salmo salar:Histochemistry and responses to compound 48/80 and formalin-inactivated Aeromonas salmonicida[J].Diseases of Aquatic Organisms,1994,20:95-100. REITE O B.Mast cells/eosinophilic granule cells of teleostean fish:A review focusing on staining properties and functional responses[J].Fish Shellfish Immunol,1998,8:489-513. REITE O B,EVENSEN φ.Inflammatory cells of teleostean fish:A review focusing on mast cells/eosinophilic granule cells and rodlet cells[J].Fish Shellfish Immunol,2006,20:192-208. ZHUANG X,SILVERMAN A J,SILVER R.Distribution and local differentiation of mast cells in the parenchyma of the forebraln[J].The Journal of Comparative Neurology,1999,408:477-488. 阮幼冰,官阳,武忠弼,等.人体及动物不同组织中肥大细胞颗粒超徽结构的比较研究[J].电子显微学报,2001,20(6):763-766. HUNT T C,CAMPBELL A M,ROBINSON C,et al.Structural and secretory characteristics of bovine lung and skin mast cells:Evidence for the existence of heterogeneity[J].Clinical and Experimental Allergy,1991,21:173-182. WEIDNER N,AUSTEN K F.Evidence for morphologic diversity of human mast cells.An ultrastructural study of mast cells from multiple body sites[J].Lab Inve,1990,63(1):63-72. TUNG W.Mast cells in the chick digestive tract:Ⅱ.Fixation,distribution,histochemistry and uhrastructure[J].Tokai J Exp Clin Med,1991,16(1):27-32. VALSALA K V,L(A)RPLID B,HANSEN H J.Distribution and ultrastructure of mast cells in duck[J].Avian Diseases,1986,30(4):653-657. REITE O B.The mast cell nature of granule cells in the digestive tract of the pike,Esox lucius:Similarity to mammalian mucosal nutst cells and globule leucocytes[J].Fish Shellfish Immunol,1996,6:363-369. CAULFIELD J P,LEWIS R A,HEIN A,et al.Secretion in dissociated human pulmonary mast cells.Evidence for solubilization of granule contents before discharge[J].The Journal of Cell Biology,1980,85:299-312. NISHIGAKI T.Mast cell degranulation and its inhibition by an anti-allergic agent tranilast.An electron microscopic study[J].Virchows Arehiv B Cell Pathol,1988,55:311-322. DVORAK A M,KISSELL S.Granule changes of human skin mast cells characteristic of piecemeal degranulation and associated with recovery during wound healing in situ[J].Journal of Leukocyte Biology,1991,49:197-210.
点击查看大图
计量
- 文章访问数: 705
- HTML全文浏览量: 12
- PDF下载量: 1056
- 被引次数: 0