条斑紫菜外生细菌的遗传多样性

杨锐; 方文雅; 单媛媛; 陈海敏; 孙雪; 叶央芳

条斑紫菜外生细菌的遗传多样性

宁波大学应用海洋生物技术教育部重点实验室, 浙江宁波, 315211;宁波大学海洋生物工程重点实验室, 浙江宁波, 315211

基金项目: 国家自然科学基金资助(40776077);宁波市科技项目(2005C100023,2006A10082)

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出版历程
- 收稿日期: 2007-07-18
- 修回日期: 2008-06-02

Genetic diversity of epiphytic bacteria in Porphyra yezoensis

Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education, Ningbo 315211, China;Marine Biotechnology Laboratory (NingBo University), Zhejiang Province, Ningbo 315211, China

摘要

摘要: 为研究藻际微生物多样性与紫菜健康养殖的关系,从江苏南通(养殖)、山东青岛(野生)、浙江温州(养殖)三地的条斑紫菜叶状体和江苏东台条斑紫菜贝壳丝状体及其周围的海水样品中分离了条斑紫菜外生细菌。根据菌落特征和菌体形态将获得的63株细菌分为21种表型。16SrDNA-V3片段DGGE分析结合序列测序比对结果表明:这些菌株与假交替单孢菌(Pseudoalteromonas)、嗜冷单胞菌(Psychrobacter)和芽孢杆菌(Bacillus)等10属相应菌株具有较高的同源性。通过对条斑紫菜外生细菌区系多样性分析得出:条斑紫菜藻体外生细菌组成与生活环境相关,但不同于周围海水细菌群落;不同来源(地点)、不同生理状况(健康与病烂)和不同生长阶段(叶状体和丝状体)的条斑紫菜外生菌种群组成差异较大。许多外生细菌对常见测试菌、海水养殖动物致病菌和人致病菌具有抑菌活性,其抑菌特点与紫菜生理性状相关。从健康条斑紫菜样品分离的外生菌中假交替单孢菌占优势,病烂紫菜未分离到假交替单孢菌,提示假交替单孢菌可能与紫菜健康生长有密切关系。
- 条斑紫菜 /
- 外生细菌 /
- 遗传多样性 /
- 抑菌活性
Abstract: Epiphytic bacteria were isolated from Porphyra yezoensis cultivated in the fields or wildly distributed in natural coasts from Nantong (Jiangsu);Qingdao (Shandong) and Wenzhou (Zhejiang) in China,and the conchocelis cultured in Dongtai (Jiangsu) in order to investigate their diversity and the relationship with healthy culture of Porphyra.On the basis of morphological observation,DGGE of 16S rDNA-V3 fragments and 16S rDNA sequence,63 isolated strains were grouped into 21 morphotypes,which had high similarities with strains of 10 genus such as Pseudoalteromonas,Psychrobacter,Bacillus and so on.The analysis showed that the epiphytic bacteria flora in P.yezoensis were relevant with its growth habitats but different from its surroundings.The flora of epiphytic bacteria from different origins,different physiological status (health and disease) and different development stages (thallus and conchocelis) were quite various.The bioactivities of the epiphytic bacteria were measured by the normal tested bacteria and pathogenicbacteria of animal aquaculture or human beings,which showed correlation with the physiological status of their hosts Dominating bacteria from healthy Porphyra belonged to the genus Pseudoalteromonas,which were not found in rotted samples.The significant difference of epiphytic bacterial flora might have some relationships with the healthy growth of P.yezoensis.
- Porphyrayezoensis /
- Epiphytic bacteria /
- Genetic diversity /
- bioactivity

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参考文献(36)

BELL W,MITCHELL R.Chemotactic and growth responses of marine bacteria to algal extracellular products[J].The Biological Bulletin,1972,143:265-277.

BRUNBERG A K.Contribution of bacteria in the mucilage ot Microcystis spp.to benthic and pelagic bacterial production in a hypereutrophic lake[J].FEMS Microbiology Ecology,1999,29(1):13-22.

SUSOKO,S T.Effects of co-existence bacteria on the growth of Chattonella marina in nonaxenic culture[J].Fish Sci,1996,62:210-214.

OHTA S,CHANG T,IKEGAMI N,et al.Antibiotic substance produced by a newly isolated marine microalga,Chlorococcum HS 101[J].Bull Environ Contem Toxicol,1993,50(2):171-178.

KUBANEK J,JENSEN P R,KEIFER P A,et al.Seaweed resistance to microbial attack:a targeted chemical defense against marine fungi[J].Proceedings of the National Academy of Sciences,2003,19:1-6.

WORM S M.Dynamics of heterotrophic bacteria attached to Mictacystis spp(Cyanobacteria)[J].Aquatic Microbial Ecology,1998,14(1):19-28.

MAYALI X,DOUCETTE G J.Microbial community interactions and population dynamics of an algicidal bacterium active against Karenia brews(Dinophyeeae)[J].Harmful Algae,2002,1:277-293.

KEIJI W,NORITAKA T,HIDEKI A,et al.Symbiotic association in Chlorella culture[J].FEMS Microbiology Ecology,2005,51:187-196.

HODSON M,CROFT E,DEERY A,et al.Algae acquire vitamin B12 through a symbiotic relationship with bacteria[J].Comparative biochemistry and physilolgy part A:Molecular &Integrative physilolgy,2007,146(4):222.

MANAGE P M.KAWABATA Z,NAKANO S.Dynamics of Cyanophae-like particles and algieidal bacteria causing microcystis aeruginosa mortality[J].Limnology,2001,2(2):73-78.

林伟,陈驭,刘秀云.海洋微藻除菌及除菌与自然带菌微藻生长特点的比较[J].海洋与湖沼,2000,31(6):647-652.

苏秀榕,秦松,骆其君,等.浙江沿海藻类共生细菌的生理生化特性研究[J].生态环境,2005,14(2):239-241.

陈国耀,沈怀舜,朱庙先,等.对条斑紫菜叶状体附生菌及病原菌初步调查[J].水产养殖,1999(1):17-19.

ARASAKI S,AKIN0 K,TOMIYAMA Y A.Comparison of some physiological aspects in marine Pythium on the host and on the artificial medium[J].Bull Misaki Mar Biollnst Kyoto Univ,1968,12:203-206.

马家海.江苏省南部沿海条斑紫菜壶状菌病的调查研究[J].上海水产大学学报,1992,1(3):185-188.

马家海.条斑紫菜赤腐病的初步研究[J].上海水产大学学报,1996,5(1):17.

丁怀宇.紫菜腐霉侵染条斑紫菜叶状体过程研究[J].淮阴师范学院学报(自然科学版).2006,5(1):69-73.

徐丽宁,杨锐.紫菜病害及防治[J].水利渔业,2005,25(6):103-105.

徐涤,秦松,庞国兴.青岛和大连海区海带共附生细菌的16SrRNA基因序列分析[J].高技术通讯,2004(2):81-86.

GROSSART HP,CZUB G,SIMON M.Algae-bacteria interactions and their effects on aggregation and organic matter flux in the sea[J].Environmental Microbiology,2006,8(6):1074-1084.

向廷生,张凡.PCR-DGGE方法分析原油储层微生物群落结构及种群多样性[J].生态学报,2005,25(2):237-242.

HOLMSTROM C,KJELLEBERG S.Marine Pseudoalteromonas species are associated with higher organisms and produce biological actire extracellular agents[J].FEMS Microbiol Ecol,1999,30:285-293.

SHABIR A,DAR,J,GIJS KUENEN,et al.Nested PCR-denaturing gradient gel electrophoresis approach to determine the diversity of sulfate-reducing bacteria in complex microbial communities[J].Applied and environmental microbiology,2005,2325-2330.

姜健,杨宝灵,元起,等.海洋共附生微生物的分离和抗菌活性鉴定[J].中国海洋药物杂志,2005,24(3):39-42.

FISHER M M,WILCOX LW,Graham LE.Molecular characterization of epiphytic bacterial communities on Charophycean green algae[J].Appl Eviron Mierobiot,1998,64(11):4384-4389.

KONG M K,CHAN K.Y.A study on the bacterial flora isolated from algae[J].Bot marine,1979,22:83-97.

丁美丽,高月华.环境因子对海藻表面细菌区系的影响[J].海洋湖沼通报,1981(2):32-36.

BOLINCHES J,LEMOS ML,BARJA L.Population dynamics of heterotrophie bacterial communities associated with Fucus vcsiculosus and Ulva rigida in an estuary[J].Micorbial Ecology,1988,15(3):345-357.

徐涤.海带外生菌的初步研究[D].青岛:中国科学院海洋研究所,2003.

闫咏,马家海,许璞,等.1株引起条斑紫菜绿斑病的柠檬假交替单胞菌[J].中国水产科学,2002,9(4):12.

VERA J,ALVAREZ R,MURANO E,et al.Identification of a marine agarolytic Pseudoaheromonas and characterization of its extracellular agarase[J].Appl Environ Microbiol,1998,64(11):4378-4383.

LEE S O,KATO J,TAKIGUCHI N,et al.Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp.strain A28[J].Appl Environ Microbiol,2000,66(10);4334-4339.

MITSUTANI A,YAMASAKI I,KITAGUCHI H,et al.Analysis of algicidal proteins of adiatomlytic marine bacterium Pseudoalteromonas sp.strain A25 by two-dimensional electrophoresis[J].Phycologia,2001,40(3);286-291.

PETER D.STEINBERG.ROCKY de N.Chemical mediation of colonizartion of seaweed surfaces[J].Phycol,2002,38:621-629.

CORSARO M M,LANZETTA R,PARRILLI E,et al.Influence of growth temperature on lipid and phosphate contents of surface polysaccharides from the antarctic bacterium Pseudoalteromonas haloplanktis TAC 125[J].Journal of Bacteriology,2004,186(1):29-34.

席宇,朱大衡,刘红涛,等.假交替单胞菌及其胞外生物活性物质研究进展[J].微生物学通报,2005,32(3):108-112.

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