台湾海峡多管水母属——新种及基于线粒体COI序列分析鉴定多管水母

郑连明; 林元烧; 李少菁; 曹文清; 许振祖; 黄加祺

台湾海峡多管水母属——新种及基于线粒体COI序列分析鉴定多管水母

厦门大学海洋系, 福建厦门, 361005

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出版历程
- 收稿日期: 2007-07-03
- 修回日期: 2008-05-23

Morphological and molecular evidences of Aequorea taiwanensis n. sp. from Taiwan Strait, with mtCOI sequence analysis for genus Aequorea

Department of Oceanography, Xiamen University, Xiamen 361005, China

摘要

摘要: 报道了中国台湾海峡多管水母属一新种——台湾多管水母(Aequorea taiwanensis n.sp.),详细描述其形态特征,并基于线粒体COI基因片段序列,比较、分析了该新种和乳突多管水母、锥状多管水母的序列差异及遗传距离。形态学和分子学数据都支持台湾多管水母为多管水母属有效种。3种多管水母mtCOI基因片断序列在种间存在明显的多态性,3种多管水母的种间序列差异为9.10%~11.9%,种间遗传距离为0.097~0.130,说明该基因片段适用于多管水母属种、种以下阶元及地理种群甄别和种间系统进化学分析。结合其他学者研究结果,认为mtCOI序列差异10%~20%可以作为多管水母属及其他水螅水母纲动物同属种间差异的标准。
- 多管水母属 /
- 新种 /
- 台湾多管水母 /
- 线粒体 /
- DNA /
- COI /
- 基因 /
- 序列
Abstract: Aequoreataiwanensis n.sp.,a new species of Aequorea:from Minnan-Taiwan Shoal fishing ground upwelling region was described in this paper.Mitochondrial cytochrome oxidase subunit Ⅰ (mtCOI) gene fragments of A.taiwanensis,A.papillata and A.conica was successfully amplified by universal primers,and obtained 538 bp sequences.Sequence divergence and genetic distance was analysed based on the mtCOI gene sequences.Both morphological and molecular data supported that A.taiwanensis n.sp is a valid species.The mtCOI sequences from the three speices of genus Aequorea showed high variation frequency,with sequence divergences ranging from 9.10% to 11.9% and pairwise genetice distances ranging from 0.097 to 0.130.mtCOI sequence analysis proved to be diagnostic molecular systematic characteristics for accurate identification and discrimination of the species of Aequorea or its population,and will be used to resolved evolutionary relationships among species of Aequorea.We suggested that a difference of from 10 to 20% between mtCOI sequences indicated species-level divergence among cogeneric species in Hydromedusa.Morphological description of Aequoreataiwanensis n.sp.:Usually 25 mm wide.Umbrellaplatter than a hemisphere,lens-shaped,with solid jelly,jelly thicker in centre,thinning gradually and evenly towards umbrella margin;manubrium broad and large,about one-half diameter of umbrella;lateral walls very extensive,with transparent lines radiating from ends of radial canals to mout-hlips;mouth-lips elongated,slender-shaped,usually approximately as numerous as radial canals.Radial canals straight and narrow,usually 90~102 in number.Ring canal narrow;gonads linear,extending along almost whole length of radial canals.With 12 marginal tent acles,with elongated conical bases narrowing suddenly distally,with a short adaxial excretory papillae,scattering black pigments.With 3~5 small,marginal bulbs between marginal tentacles with adaxial excretory papillae,but without black pig ments.With one statocysts between successive radial canals,each with 1~2 concretions,velum narrow.
- Aequorea /
- new species /
- Aequoreataiwanensis n. sp. /
- mtCOI /
- sequence /

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