白斑综合症病毒膜蛋白VP124参与病毒的感染

朱艳冰; 吴成林; 杨丰

白斑综合症病毒膜蛋白VP124参与病毒的感染

1.
集美大学, 生物工程学院, 福建, 厦门, 361021
2.
国家海洋局, 第三海洋研究所海洋生物遗传资源重点实验室, 福建, 厦门, 361005

基金项目: 国家“863”资助项目(2003AA626020);福建科学基金资助项目(2003F001)

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出版历程
- 收稿日期: 2006-06-28
- 修回日期: 2007-06-18

White spot syndrome virus envelope protein VP124 is involved in the virus infection

1.
School of Biotechnology, Jimei University, Xiamen 361021, China
2.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

摘要

摘要: 白斑综合症病毒(white spot syndrome virus,WSSV)是对虾的最主要病毒病原之一,给对虾养殖造成了巨大的经济损失。WSSV分别与三种该病毒膜蛋白VP39,VP124和VP187的特异性抗血清在体外作用后,再注射到螯虾体内,进行中和试验。结果显示,注射同或未同VP124抗血清作用的WSSV,8 d后螯虾的死亡率分别是100%和60%,WSSV的感染能够被抗VP124抗体中和。进一步利用定量PCR分析上述三种特异性抗血清对病毒感染的影响,结果显示,VP124抗血清可抑制WSSV在螯虾体内的增殖。所有结果表明,VP124在病毒的感染中起作用。
- 白斑综合症病毒 /
- 膜蛋白VP124 /
- 抗血清 /
- 感染 /
- 中和试验
Abstract: White spot syndrome virus (WSSV) is one of the major shrimp pathogens causing large economic losses for shrimp farming.In an attempt to identify the envelope proteins involved in the virus infection, purified WSSV virions were mixed with three antisera against WSSV envelope proteins (VP39, VP124 and VP187), individually.And then they were injected intramuscularly into crayfish to conduct in vivo neutralizationassays.The results showed that for groups injected with virions only and groups injected with virions and the VP124 antiserum mixture, the crayfish mortalities were 100% and 60% at 8 day spost-infection, individually.The virus infection could be neutralized by antibody against the envelope protein VP124.Quantitative PCR were used to further investigate the influence of three antisera above described on the virus infection.The results showed that the VP124 antiserum couldrestrainthe propagation of WSSV in cray fish.All of the results suggested that the viral envelope protein VP124 played a role in WSSV infection.
- white spot syndrome virus /
- envelope protein VP124 /
- antibody /
- infection /
- neutralization assay

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参考文献(16)

CHOU Hsin-yiu,HUANG Chang-yi,WANG Chung-hsiung,et al.Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan[J].Dis Aquat Org,1995,23:165-173.

DURAND S,LIGHTNER D V,REDMAN R M,et al.Ultrastructure and morphogenesis of white spot syndrome baculovirus (WSSV)[J].Dis Aquat Org,1997,29:205-211.

YANG Feng,HE Jun,LIN Xiong-hui,et al.Complete genome sequence of the shrimp white spot bacilliform virus[J].J Virol,2001,75,11811-11820.

CHIU Wen-ling,CHANG Wen.Vaccinia virus J1R protein:a viral membrane protein that is essential for virion morphogenesis[J].J Virol,2002,76:9575-9587.

CHAZAL N,GERLIER D.Virus entry,assembly,budding,and membrane rafts[J].Microbiol Mol Biol Rev,2003,67:226-237.

VANHULTEN M C,WITTEVELDT J,SNIPPE M,et al.White spot syndrome virus envelope protein VP28 is involved in the systemic infection of shrimp[J].Virology,2001,285:228-233.

SCHOFIELD D J,GLAMANN J,EMERSON S U,et al.Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein[J].J Virol,2000,74:5548-5555.

WU Wen-lin,WANG Lei,ZHANG Xiao-bo.Identification of white spot syndrome virus (WSSV) envelope proteins involved in shrimp infection[J].Virology,2005,332:578-583.

HUANG Ru,XIE Yun-li,ZHANG Jian-hong,et al.A novel envelope protein involved in white spot syndrome virus infection[J].J Gen Virol,2005,86:1357-1361.

LI Li,XIE Xi-xian,YANG Feng.Identification and characterization of a prawn white spot syndrome virus gene that encodes an envelope protein VP31[J].Virology,2005,340:125-132.

ZHU Yan-bing,LI Hong-yan,YANG Feng.Identification of an envelope protein (VP39) gene from shrimp white spot syndrome virus[J].Arch Virol,2006,151:71-82.

ZHU Yan-bing,XIE Xi-xian,YANG Feng.Transcription and identification of a novel envelope protein (VP124) gene of shrimp white spot syndrome virus[J].Virus Res,2005,113,100-106.

LI Hong-yan,ZHU Yan-bing,XIE Xi-xian,et al.Identification of a novel envelope protein (VP187) gene from shrimp white spot syndrome virus[J].Virus Res,2006,115:76-84.

YANG Feng,WANG Wei,CHEN Rong-zhong,et al.A simple and efficient method for purification of prawn baculovirus DNA[J].J Virol Methods,1997,67:1-4.

WANG Wei,HE Jun,YANG Feng,et al.Detection of prawn white spot baculovirus by polymerase chain reaction[J].Acta Oceanol Sin,1997,18:591-599.

XIE Xi-xian,LI Hong-yan,XU Li-mei,et al.A simple and efficient method for purification of intact white spot syndrome virus(WSSV) viral particles[J].Virus Res,2005,108:63-67.

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