甾醇在海洋微藻中的分布研究

徐继林; 严小军; 周成旭; 马斌

甾醇在海洋微藻中的分布研究

宁波大学, 浙江省海洋生物工程重点实验室, 浙江, 宁波, 315211

基金项目: 国家自然科学基金(20472040);科技部中国欧盟科技合作项目(国科外[2005]262-0505);浙江省自然科学基金(y504083)

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出版历程
- 收稿日期: 2006-03-28
- 修回日期: 2006-11-08

Studies on sterol distribution in marine microalgae

Key Laboratory of Marine Biotechnology, Ningbo Uiversity, Ningbo 315211, China

摘要

摘要: 对29种(株)海洋微藻进行不充气培养,用Bligh-Dyer法提取总脂,皂化后氯仿和正己烷(体积比为1:4)提取甾醇,BSTFA衍生后用岛津QP2010气相色谱-质谱分析仪进行GC/MS分析.从29种海洋微藻中共检测到47种甾类化合物,鉴定出36种甾醇和一种甾酮.其中,4位无甲基Δ⁵甾醇在所有的微藻中均有分布,一些高等生物中常见的甾醇如胆甾醇、菜子甾醇、菜油甾醇、豆甾醇、谷甾醇和褐藻中常见的岩藻甾醇等,在微藻中均有广泛的分布.相对而言,4位无甲基5位饱和甾(烷)醇、4-甲基甾(烷)醇和双羟基甾醇的分布则相对较少,其中许多甾醇如甲藻甾醇、巴夫甾醇、C_27:2(Δ^5,22/24Me)和C_28:3(Δ^5,7,22/24Me)等,只在一种藻或同一纲的微藻中被检测到,可以作为对应微藻或对应纲微藻的特征甾醇.认为海洋微藻甾醇的研究可以为海洋微藻化学分类学及饵料营养学研究提供重要参考依据.
- 海洋微藻 /
- 甾醇 /
- 化学分类学
Abstract: Total lipids were extracted using Bligh-Dyermethod from 29 species/strains of marine microalgae which had been harvested from suspended culture. Sterols were isolated by solvent partition with chloro-form :hexane(V/V, 1:4), after saponification. Sterols after BSTFA derivatization were subject to GC) MS analysis using Shimadzu QP2010 gas chromatography mass spectrometer. Forty seven sterols had been detected from the 29 marine microalgal species/strains, among which, 36 sterols and one sterolket one had been identified according to mass spectrometric characterization. After careful study, we had found some distribution features for sterols in the marine microalgae. 4-Desmethyl Δ⁵ sterols existed almost ubiquitously in the marine microalgae, including some commonsterols in higher plants such as cholesterol, brassicasterol, campesterol, stigmasterol, sitosterol, as well as fucosterol, a major sterol in the kelp. However, distribution of 4-desmethyl 5-saturated sterols, 4-methyl sterols and dihydroxyl sterols were limited in some certain species, among which, dinosterol, pavlovol, C_27:2(Δ^5,22/24Me) and C_28:3(Δ^5,7,22/24Me) were only detected in one species or belong to the same class, therefore, which could be considered as biomarker sterols. The results obtained in this paper could be provided as useful database in chemotaxo no mic studies and nutritional studies in marine microalgae.
- marine microalgae /
- sterols /
- chemotaxonomy

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参考文献(21)

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