病原性溶藻弧菌对大黄鱼鳃黏液黏附作用研究

鄢庆枇; 陈强; 马甡; 庄峙厦; 王小如

病原性溶藻弧菌对大黄鱼鳃黏液黏附作用研究

1.
集美大学, 水产学院, 福建, 厦门, 361021;国家海洋局, 第一海洋研究所, 山东, 青岛, 266061
2.
集美大学, 水产学院, 福建, 厦门, 361021;中国海洋大学, 水产学院, 山东, 青岛, 266003
3.
中国海洋大学, 水产学院, 山东, 青岛, 266003
4.
国家海洋局, 第一海洋研究所, 山东, 青岛, 266061

基金项目: 国家863计划项目(2002AA639600);福建省科技项目(2006F5066);福建省高校水产科学技术与食品安全重点实验室开放基金(2007J101)

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出版历程
- 收稿日期: 2007-01-28
- 修回日期: 2007-03-28

Adhesion of pathogenic Vibrio alginolyticus to the gill mucus of Pseudosciaena crocea

1.
Fisheries College, Jimei University, Xiamen 361021, China;First Institute Of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Fisheries College, Jimei University, Xiamen 361021, China;Fisheries College, Ocean University of China, Qingdao 266003, China
3.
Fisheries College, Ocean University of China, Qingdao 266003, China
4.
First Institute Of Oceanography, State Oceanic Administration, Qingdao 266061, China

摘要

摘要: 采用3H-TdR同位素示踪方法研究了溶藻弧菌对大黄鱼鳃黏液黏附作用.结果表明,溶藻弧菌的黏附量随着菌浓度的增加而升高并在180 min后达到饱和;在温度15~30℃、pH偏酸条件下黏附作用较强,Na⁺是溶藻弧菌黏附作用必需的,Ca²⁺起着辅助作用;溶藻弧菌经营养饥饿、热处理及高碘酸处理后黏附作用有明显下降;8种碳水化合物都有不同程度的促进溶藻弧菌的黏附作用,其中葡萄糖、甘露糖、果糖及麦芽糖有明显促进作用.研究结果表明:海水中的溶藻弧菌可很好地黏附于大黄鱼鳃黏液,其黏附作用受环境因子影响,并且与溶藻弧菌的表面碳水化合物、热敏结构等有着密切关系.
- 溶藻弧菌 /
- 黏附作用 /
- 大黄鱼 /
- 鳃黏液
Abstract: Adhesion of Vibrio alginolyticus to the gill mucus of Pseudosciaena crocea had been investigated using [methyl-³H] thymidine as isotope tracer. The results showed that:the adhesive quantity of V. alginolyticus increased with bacterial concentrations and reached equilibrium after incubated for 180 min;the high eradhesive quantity was obtained at 15~30℃ and sourish conditions;adhesion of V.alginolyticus could not achieved wit hout Na⁺,and Ca²⁺ played an auxiliary role in bacterial adhesion;adhesion of V. alginolyticus was inhibited remarkably by starvation, heat treat ment and periodic acid treatment;all of the 8 kinds of carbohydrates investigated enhanced the adhesion of V. alginolyticus to the gillmucus of P. crocea, among them, glucose, mannose, fructose and maltose showed the specially enhanced adhesion. The results indicated that V. alginolyticus could adhere to the gill mucus of P. crocea facilely in seawater, this bacterial adhesion was influenced by environmental factors and closely relation with superficial carbohydrate structures and some heat-sensitive structures.
- Vibrio alginolyticus /
- adhesion /
- Pseudosciaena crocea /
- gill mucus

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参考文献(24)

鄢庆枇,王军,苏永全,等.网箱养殖大黄鱼弧菌病研究[J].集美大学学报(自然科学版),2001,6:191-196.

王军,苏永全,张朝霞,等.闽南地区养殖大黄鱼细菌性疾病的病原生物学研究[J].厦门大学学报(自然科学版).2001,40(1):85-91.

CARLI A,PANE L,CASARETO L,et al.Occurrence of Vibrio alginolyticus in Ligurian coast rock pools(Tyrrhenian Sea,Italy) and its association with the copepod Tigriopus fulvus(Fisher 1860)[J].Applied and Environmental Microbiology,1993,59:1 960-1 962.

MARCO-NOALES E,MILA M,FOUZ B,et al.Transmission to Eels,Portals of Entry,and Putative Reservoirs of Vibrio vulnificus Serovar E(Biotype 2)[J].Applied and Environmental Microbiology,2001,67(10):4 717-4 725.

ELLIS A E.Innate host defense mechanisms of fish against viruses and bacteria[J].Developmental and Comparative Immunology,2001,25:827-839.

OFEK I,HSTY D L,SHARON N.Anti-adhesion therapy of bacterial diseases:prospects and problems[J].FEMS Immunology and Medical Microbiology,2003,38(3):181-191.

LUMSDEM J S,OSTLAND L E,BYRNE P J,et al.Detection of a distinct gill-surface antibody response following horizontal infection and bath challenge of brook trout Salvelinus fontinalis with Flavobacterium branchiophilum,the causative agent of bacterial gill disease[J].Diseases of Aquatic Organisms,1993,16:21-27.

BRADFORD N M.A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding[J].Annual Review of Biochemistry,1976,72:248-254.

VESTERLUND S,PALTTA J,KARP M,et al.Measurement of bacterial adhesion-in vitro evaluation of different methods[J].Journal of Microbiological Methods,2005,60:225-233.

VESTERLUND S,PALTTA J,KARP M,et al.Adhesion of bacteria to resected human colonic tissue:Quantitative analysis of bacterial adhesion and viability[J].Research in Microbiology,2005,156:238-244.

HÖRMANSDORFER S,WENTGES H,NEUGEBAUR-BÜCHLER K,et al.Isolation of Vibrio alginolyticus from seawater aquaria[J].International Journal of Hygiene and Environmental Health,2000,203:169-175.

VASCONCELOS G J,STANG W J,LAIDLAW R H.Isolation of Vibrio parahaemolyticus and Vibrio alginolyticus from estuarine areas of southeastern Alaska[J].Applied Microbiology,1975,29(4):557-559.

BORDAS M A,BALEBONA M C,ZORRILLA I,et al.Kinetics of adhesion of selected fish-pathogenic Vibrio strains to skin mucus of gilt-head sea bream(Sparus aurata L.)[J].Applied and Environmental Microbiology,1996,62:3 650-3 654.

GORDON A S,GERCKAKOV S M,UDEY L R.The effect of polarization on the attachment of marine bacteria to copper and platinum surfaces[J].Canadian Journal of Microbiology,1981,27:698-703.

HOOD M A,WINTER P A.Attachment of Vibrio cholerae under various environmental conditions and to selected substractes[J].FEMS Microbiology Ecology,1997,22:215-223.

GORDON A S,MILLERO F J.Electrolyte effects on attachment of an estuarine bacterium[J].Applied and Environmental Microbiology,1984,47:495-499.

KOGURE K,IKEMOTO E,MORISAKI H.Attachment of Vibrio alginolyticus to glass surfaces is dependent on swimming speed[J].Journal of Bacteriology,1998,180:932-937.

ATSUMI T,MCCARTER L,IMAE Y.Polar and lateral flagellar motors of marine Vibrio are driven by differention-motive forces[J].Nature,1992,355:182-184.

BELAS M R,COLWELL R R.Adsorption kinetics of laterally and polarly flagellated Vibrio[J].Journal of Bacteriology,1982,151:1 568-1 580.

BALEBONA M C,MORINIGO M A,FARIS A,et al.Influence of salinity and pH on the adhesion of pathogenic Vibrio strains to Sparus aurata skin mucus[J].Aquaculture,1995,132:113-120.

CLEMENTS M O,FOSTER S J.Starvation recovery of Staphylococcus aureus 8325-4[J].Microbiology,1998,144:1 755-1 763.

张庆华,陆承平.绿色荧光蛋白标记的嗜水气单胞菌在越冬水体的饥饿存活及复苏[J].水生生物学报,2002,26(5):465-471.

OFEK I,DOYLE R J.Bacterial adhesion to cells and tissues[M].New York:Chapman &Hall,1994.

ACORD J,MASKELL J,SEFTON A.A rapid microplate method for quantifying inhibition of bacterial adhesion to eukaryotic cells[J].Journal of Microbiological Methods,2005,60:55-62.

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