8种抗生素对塔玛亚历山大藻生长的影响

张冬宝; 隋正红; 茅云翔; 郭浩; 臧晓南; 张学成; 吕英刚

8种抗生素对塔玛亚历山大藻生长的影响

1.
中国海洋大学生命科学与技术学部海洋生物遗传与种质工程实验室, 山东青岛 266003
2.
国家海洋局海洋环境监测中心, 辽宁大连 116023
3.
青岛国风药业股份有限公司, 山东青岛 266510

基金项目: 瑞典国防科学基金课题资助(A/3864-1)

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出版历程
- 收稿日期: 2005-08-05
- 修回日期: 2006-03-20

Study on the sensitivities of eight antibiotics on Alexandrium tamarense (Lebour) Balech

1.
Laborotary of Marine Genetics and Breeding, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China
2.
National Marine Environmental Monitoring Center, State Oceanic Administration, Dalian 116023, China
3.
Qingdao Growful Pharmaceutical Corpouation Lat., Qingdao 266510, China

摘要

摘要: 对一株有毒的塔玛亚历山大藻(Alexandrium tamarense(Lebour) Balech)进行了8种抗生素-氯霉素、红霉素、林可霉素、庆大霉素、金霉素、氨苄青霉素、新霉素和链霉素的敏感性实验.结果表明,该藻对抗生素较为敏感.其中的5种:氯霉素、红霉素、林可霉素、庆大霉素和金霉素,对藻8d比生长速率的EC₅₀分别为2.45μg/cm³,25.2μg/cm³,7.95μg/cm³,285u/cm³,58.7μg/cm³,可用作该藻基因工程的选择压力,其对应的抗性基因可以作为基因工程阳性选择标记基因,应用于塔玛亚历山大藻的遗传操作研究.新霉素和链霉素对A.tamarense的生长无明显抑制作用,可以用于藻株无菌化培养而应用于甲藻产毒机制和赤潮爆发机理的研究,还应用筛选出的抗生素对藻培养物进行了除菌实验.
- 抗生素 /
- 基因工程 /
- 塔玛亚历山大藻 /
- 无菌化 /
- 选择标记
Abstract: The sensitivities of a toxic strain of Alexandrium tamarense(Lebour) Balech to eig ht antibiotics:chloram phenicol(Cm),erythromy cin(Er),lincomy cin(Lm),gentamicin(Gm),auromycin(Aum),ampicillin(Amp),neomycin(Nm) and strepto mycin(Str) were reported in the present studies.The result revealed that this dinoflag ellate was sensitive to five of the eight antibiotics.The EC50(50% effective concent ration) of specific growth rate after 8 days were 2.45 Lg/cm³,25.2 Lg/cm³,7.95 Lg/cm³,285 u/cm³ and 58.7 Lg/cm³ for Cm,Er,L m,Gm and Aum respectively.The five antibiotics were applicable as selective reagents,and the correspondent genes maybe employed as positive selective markersing enetic engineering of Alexandrium tamarense.On the contrary,Nm and Strhad noinhibition effects on the growth of the algae.These two antibiotics could be used in sterile purification of A.tamarense for clarifying the mechanism of the toxigenicity and the out break of the red tide.The inhibition effect of the selected antiobiotics were further tested.
- Alexandrium tamarense /
- antibiotics /
- genetic engineering /
- selective marker /
- strain sterilization

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