栉孔扇贝大量死亡的流行病学调查研究
Epizootiological study on mass mortality of the cultured scallop Chlamys farreri
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摘要: 2001~2003年,对养殖的栉孔扇贝进行了较系统地流行病学调查研究.以栉孔扇贝养殖集中海区长岛县亚美扇贝养殖场为定点调查和样品采集点.调查内容主要包括栉孔扇贝各养殖期的死亡率和可疑病原生物检测,同时对pH、溶解氧、温度、盐度等环境理化因子进行连续监测.调查结果显示:栉孔扇贝在养殖初期幼贝阶段死亡率较低,随后死亡率逐渐升高,至8月24日月死亡率达最高点71%.9月份以后死亡率开始下降.至10月份累积死亡率达90%以上.对环境理化因子的调查显示,pH、溶解氧、盐度的变化幅度不大,与扇贝死亡率无明显相关性;温度变化与死亡率似有一定的相关性,即高温(月平均23℃以上)可能是养殖栉孔扇贝重要的环境胁迫因子.通过对可疑致病性生物因子的调查研究及病理学观察表明,栉孔扇贝在养殖过程中可感染多种微生物,其中原核微生物类立克次体(RLO)在养殖过程各生长阶段均可检出,其感染率和感染强度与扇贝死亡率呈现一致的趋势.在病原病理检测中,发现RLO的大量感染与组织细胞严重病理损伤密切相关.在病原生物检测过程中,除RLO以外还发现有类枝原体原核生物(MLO)和类病毒(VLP)寄生.RLO的人工感染实验结果说明RLO是栉孔扇贝重要的病原菌.结合感染率和死亡率、感染强度和组织病理学的相关性以及感染实验的结果分析,认为类立克次体(RLO)可能是栉孔扇贝养殖过程中大量死亡的主要致病因子.Abstract: A systemic epizootiological study on mass mortalities of the cultured scallop Chlamys farreri was carried out during the period of 2001~2003.The results showed that the accumulated mortality rate of the cultivated scallop Chlamys farreri was very high, more than 90% from April to October, and the dead peak occurred on 24 August,being about 71%.The results on investigation of environmental factors revealed that the higher water temperature, more than 23℃,might be a serious stress factor to the cultured Chlamys farreri.Several suspect pathogens infecting the scallop were found such as rickett sia-like organism (RLO),mycoplasma-like organism (MLO) and virus-like particle (VLP).The results of histological observation showed that rickett sia-like organism inclusions were common, histological widespread infectiveagent.The averaged prevalence was from 20% to 90% among cultured scallop populations with averaged sizes of 2.5~6.1 cm.The highest infection intensity of RLO in host tissues was aver aged 31 inclusions persection.The result of experiment sinfected by purified RLOs showed that RLO was a serious pathogen to the scallop.It is inference that RLO infection was positively correlated with mortality rates of the cultured populations and might be the main pathogen responsible formass mortalities of the cultured population of scallop on the basis of the observation of intensity of natural infection, histological pathology and artificial infection experiment.
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于瑞海,王如才,田传远,等.栉孔扇贝大面积死亡的原因分析及预防探讨[J].海洋湖沼通报,1998,(3):69-72. 张福绥,杨红生.山东沿岸夏季栉孔扇贝大规死亡原因分析[J].海洋科学,1999,1:44-47. 过峰,孙耀,曲克明,等.日照石臼扇贝养殖水域夏季环境调查分析[J].海洋水产研究,1999,20(1):45-51. 王秀华,王崇明,李筠等.胶州湾栉孔扇贝大规模死亡的流行病学调查[J].水产学报,2002,26(2):149-155. 王运涛,相建海.栉孔扇贝大规模死亡的原因探讨[J].海洋与湖沼,1999,30(6):770-774. 李登峰,孙敬锋,吴信忠.栉孔扇贝体内寄生的病毒的分离纯化及其形态学观察[J].海洋学报,2002,24(4),145-148. 张维翥,吴信忠.扇贝大规模死亡病原病因学研究进展及其防治策略[A].中国科协.病虫害防治绿皮书[C],2003.166-170. 张维翥,吴信忠,李登峰,等.海湾扇贝养殖过程中的流行病学调查研究[J].海洋学报,2005,27(5):137-144. 吴信忠,潘金培.热带海洋珍珠贝类立克次体病研究.组织细胞病理学研究[J].海洋学报,1999,21(2):93-98. 吴信忠,潘金培.热带海洋珍珠贝立克次体病研究,Ⅴ.超微病理学及致病机理研究[J].海洋学报,1999,21(3):113-118. 吴信忠,潘金培,江静波.热带海洋珍珠贝类立克次体(RLO)病研究,Ⅶ.大珠母贝育苗期幼虫群体RLO病流行病学研究[J].海洋学报,2002,24(6):67-75. SUSAN E F,FRANCISCO J B.Epizootiology and Pathology of Juvenile Oyster Ddisease in the Eastern Oyster,Crassostrea virginica[J].Journal of Invertebrate Pathology,2001,78,141-154. GULKA G,CHANG P W.Prokariotic infection associated with a mass mortality of the sea scallop,Placopecten magellanicus[J].J Fish Diseases.1983,6:355-364. GULKA G,PEI W C.Pathogenicity and infectivity of a Rickettsia-like organisms in the sea scallop,Placopecten magellanicus[J].J Fish Diseases,1984,8:309-318. LEIBOVITZ C.Chlamidiosis:a newly reported serious disease of larval and postmetamorphic bay scallop Argopecten irridians Lamarck[J].J Fish Diseases,1989,12:126-136. COMPS M,BONAMI J R,VAGO C.Pathology des invertebres-mise en evidence dune infection rickettsienne chez les huitres[J].Compte Rendue Hebdomadaires des Seances de lAcademie des Sciences,Series D,1977,285:427-429. COMPS M,TIGE G,DUTHOIT J,et al.Microorganisms de type rickettsien chez les huitres[M].Haliotis:Crassostrea gigas et Ostrea edulis,1979.317-321. WU Xin-zhong PAN Jin-pei.An intracellular prokaryotic microorganism associated with lesions in the oyster Crassostrea ariakensis Gould[J].Journal of Fish Diseases,2000,23:409-414. AZEVEDO C,VILLALBA A.Extracellular giant rickettsiae associated with bacteria in the gill of Crassostrea gigas (Mollusca,Bivalvia)[J].Journal of Invertebrate.1991,Pathology 58:75-81. RENAULT T,COCHENNEC N.Rickettsia-like organisms in the cytoplasm of gill epithelial cells of the Pacific oyster Crassostrea gigas[J].Journal of Invertebrate Pathology,1994,64:160-162. MEYERS T R.Endemic diseases of cultured shellfish of Long Island,New York:adult and juvenile American oysters (Crassostrea virginica) and hard clams (Mercenaria mercenaria)[J].Aquaculture,1996,22:350-330. WU Xin-zhong,PAN Jin-pei.Studies on Rickettsia-like Organism Disease of the Tropical Marine Pearl Oyster 1:The Fine Structure and Morphogenesis of Pinctada maxima Pathogen Rickettsia-like Organism[J].Journal of Invertebrate Pathology,1999,73:162-167. HINE B K.Diggles Prokaryote infections in the New Zealand scallops Pecten novaezelandiae and Chlamys delicatula[J].Dis Aquat Org,2002,50:137-144.
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