外源激素对养殖牙鲆血浆睾酮和雌二醇含量的影响研究
Study on the effects of exogenous hormone on the plasma testosterone and estradiollevels in cultured Japanese flounder
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摘要: 雄性牙鲆单独注射促黄体激素释放激素类似物(LHRH-A)(每千克质量的注射量,下同)25μg/kg或人绒毛膜促性腺激素(hCG)1 000 IU/kg,血浆睾酮(T)含量在注射后6 h大幅度降低,12h时达到最低值,以后逐渐回升;LHRH-A与hCG联合注射[LHRH-A(12.5μg/kg)+hCG(500 IU/kg)],注射后6 h血浆T含量剧烈下降,24 h时达到最低点,以后缓慢回升;对照组雄鱼血浆T含量随着采样进行而呈现降低趋势.雌鱼单独LHRH-A后,血浆雌二醇(E2)水平逐渐下降,24 h时达到最低值,30 h后略有回升;单独注射hCG后,血浆E2含量缓慢下降,30 h达到最低值;LHRH-A与hCG联合注射,注射后6 h血浆E2含量逐渐下降,24 h时达到最低点,30 h时缓慢回升.对照组雌鱼血浆E2水平也随着采样的进行而逐渐降低.结果表明:单独或联合注射LHRH-A和hCG降低了雌、雄牙鲆血浆E2和T含量,与对照组结果相同.Abstract: When male Japanese flounders were injected with alone luteinizing hormone-releasing hormone analogue(LHRH-A) (25 g/kg) or human chorionic goandotropin(hCG) (1 000 IU/kg), plasma testosterone(T) levels decreased significantly post injection 6 hour, and occurred the lowest levels post injection 12 hour, and somewhat increased postinjection 24 to 30 hour.Plasma T content declined abruptly postinjection 6 hour and appeared the least value of plasma T post injection 24 hour in that male fish injected with LHRH-A plushCG (12.5 g/kg+500IU/kg).At the same time, the plasma T levels of male fish in control group also decreased as these fish treated with LHRH-A or hCG.The plasma estradiol(E2) levels decreased significantly post injection 6 hour, and occurred the lowest levels post injection 24 hour, and somewhat increased post injection 30 hour, when female fish were injected with alone LHRH-A, whereas, the least plasma E2 levels occurred post injection 30 hour in that female fish injected with alone hCG.Plasm a E2 content decreased gradually post injection 6 hour and appeared the least value of plasma E2 postinjection 24 hour in that female fish injected with LHRH-A plus hCG.On the mean while, the plasma estradiol levels of the fish in control group also declined as these fish treated with LHRH-A or hCG.These results suggestion that the injection with alone LHRH-A or hCG or combined LHRH-A and hCG failed to increase significantly plasma testosteroneand estradiollevels, on the contrary, suppressed testosteroneand estradiolconcent ration in male and female Japanese flounder.
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Key words:
- Japanese flounder /
- Steroid /
- Reproduction
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