海洋石油降解微生物的分离鉴定

刘陈立; 邵宗泽

海洋石油降解微生物的分离鉴定

刘陈立¹,
邵宗泽^2,

1.
厦门大学, 生命科学学院, 福建, 厦门, 361005;国家海洋局, 第三海洋研究所海洋生物遗传资源重点实验室, 福建, 厦门, 361005
2.
国家海洋局, 第三海洋研究所海洋生物遗传资源重点实验室, 福建, 厦门, 361005

基金项目: 国家海洋局科技项目资助(02302);重大基础研究前期研究专项资助项目(202CCA05000);国家自然科学基金资助项目(40376041)

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出版历程
- 收稿日期: 2004-07-01
- 修回日期: 2004-10-21

Isolation and characterization of oil-degrading marine micro-organisms

LIU Chen-li¹,
SHAO Zong-ze^2
,

1.
Life Science College, Xiamen University, Xiamen 361005, China;Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
2.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China

摘要

摘要: 以柴油为惟一碳源,从胜利油田黄河码头和厦门储油码头两个海水样品中富集得到两组柴油降解菌,共9株细菌,1株真菌,它们对柴油都有降解能力.16SrDNA鉴定结果表明这9株细菌中有3株摩加夫芽孢杆菌、1株施氏假单胞菌、1株腊样芽孢杆菌、1株反硝化产碱杆菌.1株阿氏葡萄球菌、1株食烷菌和1株类似很小海旋菌的未知新菌(M-5),其中B-5对柴油的降解能力最强,已报道的食烷菌属中其他种的同源性最高,为95.2%,表明它是该属中的一个新种.实验中获得的惟一一株真菌M-3属于假丝酵母,对柴油有较强的降解和乳化能力.实验中还从B5和施氏假单胞菌M-2中扩增到了烷烃降解的限速酶烷烃单加氧酶的基因片断,其中B5编码了一种新的烷烃单加氧酶.这些菌在石油污染的海水自净中起着重要作用,可用于海洋石油污染的生物修复.
- 海洋微生物 /
- 生物降解 /
- 柴油 /
- 烷烃单加氧酶
Abstract: By enriching with diesel fUel as a sole carbon source,two microbial consortia were obtained from different marine environment samples:one,colleted from the Huanghe Dock of Shengli Oil Field,and the other,from the Oil storage Dock Xiamen.Nine bacteria and one fungus were screened out from the two samples.All of the isolates show considerable biodeg radability to diesel fuelex cept for M-5,while it shows degradability to several kinds of PAHs.Sequence analysis of 16S rDNA identifies that the 9 bacteria belong to Bacillus cereus,Pseudomonas stutzeri,Staphylococcus arlettae,Alcanivorax sp.,Alcaligenes denitrificans and alpha proteobacterium(iso late M-5),respectively.A mong them,each of isolates B-5 and M-5 belongs to a novel species.Two alkane monoxyg enase fragments ware amplified from isolate B-5 and Pseudomonas stutzeri M-2.And the fragmentret rieved from isolate B-5 seems to encode a partial novel alkane monoxyg enase.
- marine microorganisms /
- biodegradation /
- diesel fuel /
- alkane monooxygenase

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