南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

林学政; 杨秀霞; 边际; 黄晓航

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

1.
国家海洋局, 第一海洋研究所, 山东, 青岛, 266061
2.
中国海洋大学, 海洋生命学院, 山东, 青岛, 266003

基金项目: 大洋协会“十五”计划资助项目(DY105-04-04-03)

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出版历程
- 收稿日期: 2003-03-21
- 修回日期: 2003-07-31

Studies on the low-temperature lipase of psychrophilic bacterium 2-5-10-1 isolated from the deep sea of the Southern Ocean

1.
First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2.
Marine life College, Ocean University of China, Qingdao 266003, China

摘要

摘要: 从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu²⁺和Zn²⁺对酶活有着强烈的抑制作用.
- 南大洋 /
- 深海嗜冷菌 /
- 低温脂肪酶
Abstract: A strain of psychrophilic bacteria,2-5-10-1,which produces low-temperature lipase,was isolated from the deep sea of the Prydz Bay in the Southern Ocean.The optimal growth temperature of strain 2-5-10-1 is 5,it also produces more extracellular lipase at 5.Tween80 or olive oil in medium enhances the secretion of lipase.The optimal temperature and pH for lipase activity are 35 and 7.5 respectively.At 0,the lipase still has 37% relative enzyme activity.The lipase shows high thermolability,remaining less than 50% activity after incubation at 60 for 15 min.EDT A has no effect on lipase activity,indicating that the lipase activity is independent of divalentcation.In contrast,the lipase activity is inhibited markedly by Cu²⁺ and Zn²⁺.
- Southern Ocean /
- deep sea psychrophilic bacteria /
- low-temperture lipase

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