锯缘青蟹视神经节免疫细胞化学研究

黄辉洋; 李少菁; 叶海辉; 王桂忠; 李祺福

锯缘青蟹视神经节免疫细胞化学研究

1.
厦门大学海洋学系, 福建, 厦门, 361005
2.
厦门大学海洋学系, 福建, 厦门, 361005;厦门大学生命科学学院, 细胞生物学研究室, 福建, 厦门, 361005
3.
厦门大学生命科学学院, 细胞生物学研究室, 福建, 厦门, 361005

基金项目: 福建省重中之重资助项目;高等学校博士学科点专项科研基金资助项目(20010384010);国家自然科学基金资助项目(30300269)

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出版历程
- 收稿日期: 2002-07-18
- 修回日期: 2002-11-04

Immunocytochemical studies on the optic ganglia of the mud crab, Scylla serrata (Forskl)

1.
Department of Oceanography, Xiamen University, Xiamen 361005, China
2.
Department of Oceanography, Xiamen University, Xiamen 361005, China;Laboratory of Cell Biology, School of Life Science, Xiamen University, Xiamen 361005, China
3.
Laboratory of Cell Biology, School of Life Science, Xiamen University, Xiamen 361005, China

摘要

摘要: 采用兔抗哺乳类血清,应用链霉菌抗生物素蛋白-过氧化物酶免疫细胞化学技术,观察了锯缘青蟹视神经节中5羟色胺、胰高血糖素、神经肽Y和生长抑素免疫阳性细胞和神经纤维的形态和分布,结果发现,视神经节的4个神经髓均有5羟色胺免疫阳性细胞,除X器检出1个较大型阳性细胞外,其余均为小型细胞.视外髓、视内髓和视端髓都具有胰高血糖素免疫阳性细胞,视端髓的神经髓质阳性染色深,X器中阳性细胞成群分布,窦腺免疫阳性反应强.神经肽Y免疫阳性细胞在视神经层和视外髓为小型阳性细胞,在视内髓和视端髓检出较大型阳性细胞,另有小型阳性细胞散布于X器中.生长抑素免疫阳性细胞分布于4个神经髓,数量少.4种免疫阳性物的特异性分布模式,可为其不同的神经生理作用提供形态学证据.
- 锯缘青蟹 /
- 视神经节 /
- 5羟色胺 /
- 肽类 /
- 免疫细胞化学
Abstract: Immunocytochem istry technologies were applied to observe the mimunoreactive neurons and neuropils of seroton in (5-HT),glucagon (Glu),neuropeptide Y (NPY) and somatostatin (SOM) in the optic ganglia of Scylla serrata.The results are as follows:5-HT-mimunoreactive cells are located in lamina ganglionaris(LG),medulla externa(ME),medulla interna(MI) and medulla term inalis(MT).Except one large mimunoreactive cell in the Xorgan,the other mimunoreactive cells are small.The presence of Glu-mimunoreactive cells is found in the optic ganglia except LG.The mimunoreactivity is strong in the neuropils of MT.Many mimunoreactive cells in MT are aggregated in Xorgan.The sinus gland is mimunoreactive.The NPY-imm unoreactive cells are small in LG and ME and large in MI and MT.Small cells are also seen dispersing in the Xorgan.A few SOM-immunoreactive cells are distributed in the four neuropils in the optic ganglia.The specific distribution patterns of the four immuno-reactive substances may thus supply morphological proofs for their different neurophysio logical function.
- Scylla serrata /
- optic ganglia /
- serotonin /
- peptides /
- mimunocyto chemistry

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