皱纹盘鲍杂交F<sub>1</sub>代与亲本的RAPD标记及分离方式分析

肖洁; 刘晓; 张国范; 郭希明

皱纹盘鲍杂交F₁代与亲本的RAPD标记及分离方式分析

1.
中国科学院, 海洋研究所, 山东, 青岛, 266071
2.
中国科学院, 海洋研究所, 山东, 青岛, 266071;Haskin贝类研究实验室, 新泽西州立大学, 新泽西, 08349

基金项目: 国家"863"计划课题资助项目(2002AA629030;2001AA621070);国家自然科学基金资助项目(30371117;39825121).

计量
- 文章访问数: 639
- HTML全文浏览量: 10
- PDF下载量: 1054
- 被引次数: 0
出版历程
- 收稿日期: 2004-03-29
- 修回日期: 2004-07-13

Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino

1.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
2.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Haskin Shellfish Research Laboratory, Rutgers University, Port Norris, NJ 08349, USA

摘要

摘要: 应用RAPD标记技术对皱纹盘鲍杂交家系JC的双亲及3个F₁个体进行了分析,对JC家系在皱纹盘鲍遗传连锁图谱构建中的应用前景进行了初步评估,并分析了RAPD分子标记在JC家系F₁代中的分离方式及该家系的1对亲本及其子代个体的遗传结构.7条随机引物共扩增出41个分子标记,其中24个为多态性,占总数的58.5%;有14个分离标记可用于遗传连锁作图,有7个符合孟德尔独立分配定律,占总数的17.1%.2个标记与子代个体大小有一定的相关性,但其连锁关系仍有待于进一步研究.父母本之间的遗传距离为0.384 4,父本与子代间的距离为0.137 2,母本与子代间的距离为0.127 3;父本提供的分离标记在子代中的显性频率为0.47,母本为0.55.双亲之间的遗传距离较远,杂交产生的JC-F₁群体具有较高的杂合度(0.201 6),该结果初步表明皱纹盘鲍杂交家系JC的子1代即可作为作图群体应用于皱纹盘鲍的连锁图谱构建.
- 皱纹盘鲍 /
- RAPD /
- 遗传结构 /
- 分子标记 /
- 连锁图谱
Abstract: The genetic structure and segregation of molecular markers in a F₁ hybrid family of Pacific abalone(Haliotis discus hannai Ino)were studied using random amplified polymorphic DNA(RAPD)technology.This family's usefulness in linkage mapping of abalones was assessed.Seven primers yielded a total of 4.markers,of which 24 (58.5%)were polymorphic in this family,14 were informative and could be used for linkage mapping,and 7 (17.1%)markers were segregated in the expected Mendelian ratio which is very low compared with other species. Two markers show some association with JC-F₁'s phenotypes,and this relationship needs further study.The genetic distances between two parents,father and progeny,mother and progeny are 0.384 4,0.137 2,0.127 3 respectively.The frequency in JC-F₁ population of RAPD markers from father and mother are 0.824 and 0.841, which are almost the same.High genetic distance between parents and high heterozygosity(0.2016)in JC-F₁ population is also confirmd the feasibility of linkage mapping using these parents and progenies.
- Haliotis discus hannai /
- RAPD /
- genetic structure /
- molecular markers /
- linkage mapping

HTML全文

参考文献(24)

POSTLETHWAIT J H, JOHNSON S L, MIDSO C N, et al. A genetic linkage map for the zebrafish[J]. Science, 1994,264(5159): 699-703.

SHIMODA N, KNAPIK E W, ZINITI J. Zebrafish genetic map with 2000 microsatellite markers[J]. Genomics, 1999, 58(3): 219-232.

AGRESTI J J, SEKI S, CNAANI A, et al. Breeding new strains of talipia: development of a new artificial center of origin and linkage map based on AFLP and microsatellite loci[J]. Aquaculture, 2000, 185: 43-56.

YOUNG W P, WHEELER P A, CORYELL V H, et al. A detailed linkage map of rainbow trout produced using doubled haploids[J]. Genetics, 1998, 148(2): 839-850.

MORIZOT D C, SLAUGENHAUPT S A, KALLMAN K D, et al. Genetic linkage map of fishes of the genus Xiphophorus (Teleostei: Poeciliidae)[J]. Genetics, 1991, 127(2): 399-410.

SAKAMOTO T, DANZMANN R G, GHARBI K, et al. A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates[J]. Genetics, 2000, 155(3): 1 331-1 345.

LIU Z, KARSI A, LI P, et al. An AFLP-based genetic linkage map of channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family[J]. Genetics, 2003, 165(2): 687-694.

孙效文,梁立群.鲤鱼的遗传连锁图谱初报[J].中国水产科学,2000,7(1):1-5.

COIMBRAM R M, KOBAYASHI K, KORESTUGU S, et al. A genetic linkage map of the Japanese flounder, Paralichthys olivaceus[J]. Aquaculture, 2003, 220(1): 203-218.

MOORE S S, WHAN V, DAVIS G, et al. The development and application of genetic markers for the Kuruma prawn Penaeus Japonicus and their use in parentage determination and linkage mapping[J]. Aquaculture, 1999, 173: 19-32.

WILSON K, LI Y, WHAN V, et al. Genetic mapping of the black tiger shrimp Penaeus monodon with amplified fragment length polymorphisms[J]. Aquaculture, 2002, 204: 297-309.

YU Z, GUO X. Genetic linkage map of the eastern oyster Crassostrea virginica Gmelin[J]. Biol Bull, 2003, 204 (3):327-338.

李莉,郭希明.遗传图谱及其在主要水产动物的研究进展[J].海洋科学,2003,27(11):14-19.

LI L, GUO X. AFLP-based genetic linkage maps of the pacific oyster Crassostrea gigas Thunberg[J]. Marine Biotechnology, 2004, 6(1): 26-36.

万俊芬,汪小龙,潘洁,等.日本盘鲍×皱纹盘鲍子代杂种优势的RAPD分析[J].青岛海洋大学学报,2001,31(4):506-512.

张国范,王继红,赵洪恩,等.皱纹盘鲍中国群体和日本群体的自交与杂交F₁的RAPD分析[J].海洋与湖沼,2002,33(5):484-491.

张志峰,矛云翔,潘洁,等.皱纹盘鲍肌动蛋白基因启动子的克隆和序列分析[J].水产学报,2001,25(5):398-401.

刘晓,高其康,张国范.不同壳色皱纹盘鲍杂交家系J1Rh F₁全长cDNA文库的构建[J].水产学报,2004,28(1):23-28.

赵洪恩.鲍的增养殖[M].沈阳:沈阳出版社,1999.

刘振辉,孔杰,刘萍,等.RPAD技术在中国对虾群体鉴别上的应用[J].海洋学报,2003,24(4):67-71.

权洁霞,戴继勋,沈颂东,等.梭鱼人工养殖群体与自然群体的随机扩增多态DNA(RAPD)分析[J].海洋学报,2000,22(5):82-87.

沈怀舜,朱建一,丁亚平,等.我国沿海三个文蛤地理群的RAPD分析[J].海洋学报,2003,25(5):97-102.

LI Y, BYRNE K, MIGGIANO E, et al. Genetic mapping of Kuruma prawn Penaeus japonicus using AFLP markers[J].Aquaculture, 2003, 219:143-156.

GRATTAPAGLIA D, SEDEROFF R. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers[J]. Genetics, 1994, 137(4): 1 121-1 137.

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 639
HTML全文浏览量: 10
PDF下载量: 1054
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

皱纹盘鲍杂交F₁代与亲本的RAPD标记及分离方式分析

计量

Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino

计量

目录

留言板

皱纹盘鲍杂交F1代与亲本的RAPD标记及分离方式分析

计量

出版历程

Studies on segregation of RAPD markers in a F1 hybrid family and parents of Haliotis discus hannai Ino

计量

出版历程

目录

皱纹盘鲍杂交F₁代与亲本的RAPD标记及分离方式分析

Studies on segregation of RAPD markers in a F₁ hybrid family and parents of Haliotis discus hannai Ino