牙鲆养殖群体遗传结构的研究

黎中宝

牙鲆养殖群体遗传结构的研究

黎中宝

基金项目: 福建省重点科技攻关资助项目(2002N020)

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出版历程
- 收稿日期: 2003-05-20
- 修回日期: 2003-08-25

Genetic structure of cultivated stocks in Paralichthys olivaceus

LI Zhong-bao

摘要

摘要: 应用聚丙烯酰胺凝胶电泳技术研究了牙鲆养殖群体(F1代)的遗传结构.结果表明,牙鲆养殖群体具有较高的遗传多样性水平.牙鲆群体平均每位点等位基因数的变化为1.19(0.11)～1.24(0.10).多态位点百分数的变化为14.29%～23.81%.期望杂合度的变化为0.056(0.036)～0.057(0.033).观察杂合度的变化为0.099(0.065)～0.104(0.065).牙鲆群体间遗传分化较低,群体间分化度为0.005,表明总的遗传变异中有99.5%来自群体内,有0.5%来自群体间.群体间遗传距离和遗传一致度均值分别为0.001和0.999.群体间基因流很大,等于49.75.
- 牙鲆 /
- 等位酶 /
- 遗传结构 /
- 遗传多样性 /
- 遗传分化
Abstract: Genetic structure of cultivated stocks was investigated using the assay of vertical slab polyacr ylamidegel electrophoresis in Paralichthys olivaceus.Higher level of genetic diversity in the stocks was found:Mean number of alleles perlocus(A) is 1.19(0.11)~1.24(0.10),percentage of polymorphic loci(P_0.99) is 14.29%~23.81%, the observed heterozygosity(H_o) is 0.099(0.065)~0.104(0.065),expected heterozygosity(H_e) is 0.056 (0.036)~0.057(0.033).The results showed that the coefficient of gene differentiation between the stocks is low,F_st=0.005,there is no significant difference in biochemical genetic character istics among the stocks.The genetic distance and genetic identity is 0.001 and 0.999 respectively.Gene flow among the stocks is large(Nm=49.75).
- Paralichthys olivaceus /
- allozyme /
- genetic structure /
- genetic diversity /
- genetic differentiation

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参考文献(25)

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