三种海产帘蛤目贝类的核型研究
Karyotypes of three species of the marine Veneroida molluscs
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摘要: 以大竹蛏、紫石房蛤和中国蛤蜊的鳃组织为材料,经注射植物血球凝集素和秋水仙素处理,低渗作用后,采用滴片法和压片法制片,对3种贝的核型进行了研究.结果表明,3种贝类的二倍体染色体数均为38条,大竹蛏的核型为26m+6sm+2st+4t,NF=0;紫石房蛤的核型为32m+2sm+4st/t,NF=2;中国蛤蜊的核型为20m+16sm+2st/t,NF=4.在3种贝类的染色体中均未发现随体及性染色体.Abstract: The karyotypes of three species of marine Veneroida molluscs,Solen grandis Dunker,Saxidomus purpuratus Sowerby and Mactra chinensis Philippi were studied by using the adult gill tissues.The chromosomes were prepared through injection of phytohemagglutinin(PHA)and colchicine,hypotonic treatment,chopping air-dry,and squashing technology.The results show that the diploid chromosome numbers of the three Veneroida species are the same as 2n=38.The karyotype of Solen grandis is 26 m+6 sm+2 st+4 t,NF=70,of Saxidomus purpuratus is 32 m+2 sm+4 st/t,NF=72,of Mactra chinensis is 20 m+16 sm+2 st/t,NF=74. Satellite and sex chromosome were not found among the chromosomes of three species.
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Key words:
- Solen grandis Dunker /
- Saxidomus purpuratus Sowerby /
- Mactra chinensis Philippi /
- chromosome /
- karyotype
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