毛蚶血清凝集素的凝集活性

刘志鸿; 张士璀; 杨爱国; 王长法; 王清印

毛蚶血清凝集素的凝集活性

1.
中国水产科学研究院, 黄海水产研究所, 山东, 青岛, 266071;中国海洋大学, 海洋生命学院, 山东, 青岛, 266003
2.
中国海洋大学, 海洋生命学院, 山东, 青岛, 266003
3.
中国水产科学研究院, 黄海水产研究所, 山东, 青岛, 266071

基金项目: 国家重大基础研究计划资助项目(G1999012009;G1999012005);国家"863"计划资助项目(2001AA628070).

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出版历程
- 收稿日期: 2001-07-30
- 修回日期: 2001-09-21

Properties of agglutinin in the serum of bivalve Scapharca subcrenata

1.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
2.
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
3.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

摘要

摘要: 以毛蚶为实验材料,对其血清中的凝集素进行研究.毛蚶血清凝集素对人的A,B,O血型红细胞、鸡和小鼠的红细胞均有凝集作用,凝集效价分别为256,256,8,16,64,同时它还能对白色念珠菌、啤酒酵母、溶藻胶弧菌等微生物具有凝集作用.毛蚶血清凝集素具有一定的pH值稳定性和热稳定性.在选用的12种单糖和寡糖中,L-鼠李糖、L-树胶醛糖、麦芽糖、D-半乳糖、蔗糖对毛蚶血清的凝集活性有明显的抑制作用.用弧菌对毛蚶进行免疫刺激时,其血清对鸡红细胞的凝集效价明显增加.
- 毛蚶 /
- 血清 /
- 凝集素
Abstract: Agglutinin has been shown to exist in the serum of bivalve Scapharca subcrenata.The agglutinin showed agglutinating activity against human A,B,O,chicken and mouse erythrocytes,the agglutinintiters were 256,256, 8,16,64 respectively.The serum could also agglutinate microorganisms,such as Candida albicans,Sacchar omycescer evisiae,Vibrio alginolyticus.The experiment showed that the agglutinin was stable at a wide range of temperature and pH degree.The activity of the agglutinin could be inhibited by L-rhamnose,L-arabinose,maltose, Dgalactose and sucrose.When the animals were infected by Vibrio sp.,their serum showed agglutinating titers in crease.
- Scapharca subcrenata /
- serum /
- agglutinin

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参考文献(9)

TUNKIJJANUKIJ S, GIAEVER H, CHIN C C Q, et al. Sialic acid in hemolymph and affinity purigied lectins from two marine bivalves[J]. Comparative Biochemistry and Physiology (Part B), 1998, 119: 705-713.

牟海津,江晓路,刘树青,等.双壳贝类血清中凝集素凝集性能初步研究-对脊椎动物血细胞的凝集作用[J].青岛海洋大学学报,1999,29(2):249-254.

森勝羲,刀根靖幸,鈐木敏和,等.軟体動物の生体防御機構-Ⅰ.ホタテガイ組織における殺菌素と凝集素の活性[J].Bulletin ofthe Japanese of Scientific Fisheries,1980,46(6):717-722.

朱越雄,曹广力.丽文蛤血清中外源凝集素的凝集性能[J].海洋科学,2000,24(10):55-56.

TAMPLIN M L, FISHER W S. Occurrence and characteristics of agglutination of Vibrio cholerae by serum from the eastern oyster Crassostrea virginica[J]. Appl Environ Microh, 1989, 55(11): 2 882-2 887.

JOHNSEN H M. Human blood group A1 specific agglutinin of the butter clam Saxidomus giganteus[J]. Science, 1964,146(23): 548-549.

SHARON N, LIS H. Lectins: cell agglutinating and sugar-specific proteins[J]. Science, 1972, 177: 949-959.

OLAFSEN J A, FLAETCHER T C, GRANT P T. Agglutinin activity in pacific oyster (Crassestrea gigas) hemolymph following in vivo Vibrio anguillarum Challenge[J]. Developmental and Comparative Immunology, 1992, 16: 123-138.

LEIPPE M, RENWRANTZ L. Release of cytotoxic and agglutinating molecules by Mytilus hemocytes[J]. Dev Comp Immunol, 1988, 12(2): 297-308.

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