RAPD技术在中国对虾群体鉴别上的应用

刘振辉; 孔杰; 刘萍; 邓景耀

RAPD技术在中国对虾群体鉴别上的应用

1.
中国海洋大学, 海洋生命学院, 山东, 青岛, 266003
2.
中国水产科学院, 黄海水产研究所, 山东, 青岛, 266071

基金项目: 国家“973”计划资助项目(G1999012007)

计量
- 文章访问数: 688
- HTML全文浏览量: 9
- PDF下载量: 752
- 被引次数: 0
出版历程
- 收稿日期: 2000-11-07
- 修回日期: 2001-05-31

Application of RAPD technology for identification in three different stocks of Penaeus chinensis

1.
College of Marine Biology Science, Ocean University of China, Qingdao 266003, China
2.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

摘要

摘要: 采用RAPD技术对中国对虾(Penaeus chinensis)、中国黄海、渤海沿岸种群(H)朝鲜半岛西海岸种群(K)和即墨健康养殖群体(S)的DNA进行检测,41个随机引、物在3个群体间共筛选到了3个稳定的RAPD标记F-02₅₄₀,OPN-13₈₀₀和OPN-20₂₂₀₀,可用于群体间的鉴别,研究将为中国对虾分子标记遗传育种提供依据。
- 中国对虾 /
- RAPD技术 /
- 遗传标记
Abstract: Three stocks (K,H and S) of Penaeus chinensis were analysed using 41 random oligonucleotide primers with 10 bases by RAPD method.K was from the spawning stock in the western coastal waters of Korea Peninsula;H was from the Huanghai (Yellow) Sea-Bohai Sea stock;S was from the health cultured stock in Jimo,Shandong Province.Three genetic markers which can identify the three stocks were got in this study.The study may provide the basic materials for marker assisted selection (MAS) of Penaeus chinensis.
- Penaeus chinensis /
- RAPD technology /
- genetic marker

HTML全文

参考文献(7)

CARR W,SWEENEY J,SWINGLE J.The Oceanic Institute's SPF shrimp breeding program status [C].USMSFP(US Marine Shrimp Farming) 10th Anniversary Review,GCRL Special Publication,1994,(1):47-54.

PRUDER G D,BROWN C L,SWEENEY J N,et al.High health shrimp systems:seed supply theory and practice[A].BROWDY C L,HOPKINS J S.Swimming Through Troubled Waters:Proceedings of the Special Session on Shrimp Farming,1～4 February,San Diego,CA.[C].Baton Rouge,LA USA:World Aquaculture Society,1995.40-52.

WYBAN J A,SWINGLE J S,SWEENEY J N,et al.Specific pathogen-free Penaeus vannamei[J].World Aquaculture,1995,24:39-45.

邓景耀,叶昌臣,刘永昌.渤黄海的对虾及其资源管理[M].北京:海洋出版社,1990.36-164.

SAMBROOD J,FRITSCH E F,MANIATIS T.Molecular cloning[A].A Laboratory Manual:2nd ed.[M].New York:Cold Spring Harbor Laboratory Press,1989.464-467.

WILLIAMS J G K,KUBELIK A R,LIVARK K J,et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucl Acids Res,1990,18(22):6 531-6 535.

刘振辉,孔杰,石拓,等.中国对虾两个不同地理种群遗传结构的RAPD分析 [J].应用与环境生物学报,2000,6(5):440-443.

施引文献

资源附件(0)

访问统计