锯缘青蟹卵黄发生期卵巢和肝胰腺脂类的变化

成永旭; 李少菁; 王桂忠; 陈学雷; 林琼武; 徐瑾

锯缘青蟹卵黄发生期卵巢和肝胰腺脂类的变化

1.
上海水产大学渔业学院, 上海200090
2.
厦门大学海洋系, 福建厦门361005

基金项目: 福建省重中之重项目;福建省自然科学基金资助项目(C97010)

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出版历程
- 收稿日期: 1998-12-20
- 修回日期: 2000-10-30

Changes in lipid class and fatty acid compositions in the ovaries and hepatopancreas of the mud crab Scylla serrata during the vitellogensis of the second ovarian maturation

1.
Fisheries College, Shanghai Fisheries University, Shanghai 200090, China
2.
Department of Oceanography, Xiamen University, Xiamen 361005, China

摘要

摘要: 锯缘青蟹2次卵巢发育时的卵黄发生旺期与卵黄发生初期相比,卵巢指数和卵巢脂肪含量(%卵巢干重或湿重)均显著增加,积累了大量的脂类,而这一时期的肝胰腺指数和肝胰腺脂肪含量均无显著变化,说明在卵黄发生时期,肝胰腺吸收的脂肪均及时转运到正在发育的卵巢.卵黄发生期,卵巢或肝胰腺磷脂/总脂的百分含量,均无显著变化,肝胰腺中游离脂肪酸逐步增加,甚至在卵黄发生后期超过甘油三酯的含量.卵黄发生阶段,无论肝胰腺或卵巢,其磷脂长链多不饱和脂肪酸显著高于中性脂,而饱和脂肪酸含量相对较低;肝胰腺脂类脂肪酸组成的最主要变化是中性脂的n3系列的长链多不饱和脂肪酸,尤其是C22:6n3显著降低.卵黄发生初期,肝胰腺磷脂与卵巢中性脂的脂肪酸组成(肝胰腺转运脂肪时,首先要将吸收或储存的脂类转换成磷脂,才能运出)无显著差异,但卵黄发生旺期,肝胰腺磷脂的长链多不饱和脂肪酸显著高于卵巢中性脂的含量.文中讨论了这些变化的原因.
- 锯缘青蟹 /
- 卵黄发生 /
- 卵巢 /
- 肝胰腺 /
- 脂类
Abstract: From the first vitello gensis to the second vitellogensis,total lipid contents(mg/g body wet weight)and lipid concentration(% wet or dry ovarian weight)of the ovary,are significantly increased(P<0.05),and the neutral lipids(NL)and polar lipids(PL)occupy all increased total lipids,and may mainly be from the diet source,because total lipid contents and lipid concentration of the hepatopancreas are no sig nificant changes.NL[mainly triacylglycerils(TG),cholesterol and small amount of free fatty acids(FFA)]in ovaries is about 60% of total lipids, and PL(mainly phosphatidylcholine PC and phosphtidylethanolamine PE)is about 40%. During vitello gensis stages,polar lipid(mainly also PC and PE)concentration(% total lipids)in hepatopancreas is more than 30%,and NL(mainly TG,FFA and small amout of cholsterol)is less than 70%,never theless, it is notew orthy that FFA content increased during the vitellogensis,and its content is even more than that of TG in late vitellogensis.
- Scylla serrata /
- vitellogensis /
- ovary /
- hepatopancreas /
- lipid

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参考文献(21)

李少菁,林淑君,刘理东,等.锯缘青蟹卵巢发育过程中不同器官脂类和脂肪酸的组成[J].厦门大学学报(自然科学版),1994,33(增刊):109～115.

CASTILLE F L, LAWRENCE L.Relationship between maturation and biochemical composition of the gonads and digestive glands of the shrimps P.aztecus and P.setiferus[J].J Crusta Bio, 1989, 9:202～211.

LAUTIER J, LAGARRIGUE J G.Lipid metabolism of the crab Pachygrapsus marmoratus during vitellogenesis[J].Biochem Syst and Ecology, 1988, 16, 203～212.

LEE R F, WALKER A.Lipovitellin and lipid droplet accumulation in occytes during ovarian maturation in the blue crab,Callinectes sapidus[J].J Exp Zoo, 1995, 271～412.

MOURENTE G, MEDINA A, GONZALEZ S, et al.Changes in lipid class and fatty acids contents in the ovary and midgut gland of the female fiddler crab Uca tangeri during maturation[J].Mar Bio, 1994, 121:187～197.

成永旭,堵南山,赖伟.中华绒螯蟹不同发育阶段肝胰腺脂类及脂肪酸组成的变化[J].动物学报,1998,44:420～429.

MOURENTE G.In vitro metabolism of ¹⁴C-polyunsaturated fatty acids in midgut gland and ovary cells from Penaeus kerathurus at the beginng of the sexul maturation[J].Comp Biochem Physiol, 1996, 115b:255～266.

SPAARGAREN D H, HAEFNER P A.Interaction of ovary and hepatopancreas during the reproductive of Crangon crangot.II.Biochemical relationships[J].J Crus Bio, 1994, 14:6～19.

TESHIMA S, KANAZAWA A.Variation in lipid compositions suring the ovarian maturation of the prawn[J].Bull JapanSoc Sci Fish, 1983, 49:957～962.

李少菁,王桂忠,曾朝曙,等.锯缘青蟹繁殖生物学的研究[J].海洋科学,1994,2:21～24.

成永旭,堵南山,赖伟.中华绒螯蟹卵巢快速发育期内脂类积累以及对抱卵的影响[J].水产学报,2000,24:113～118.

EZHILARASI S, SUBRAMONIMA T.F sterase activity in Scylla serrata during ovarian development[J].J Exp Mar Biol Ecol, 1984, 83:1～12.

LUBSZES E, RAVIDT, KHAYAT M, et al.Isolation and characterization of the high-density lipoproteins from the hemolymph and ovary of the penseid shrimp Penaeus semisulcatus:apoproteins and lipids[J].J Exp Zoo, 1997, 278:339～348.

SPAZIANI E, HAVEL R J, HAMILTON R I, et al.Properties of serum high-density lipoproteins in the crab, Cancer antennarius timpson[J].Comp Biochem Physiol, 1986, 85B, 307～314.

成永旭,堵南山,赖伟.十足类甲壳动物卵巢发育过程中脂肪的积累与肝胰腺脂肪的变化[J].动物学杂志,1997,32.57～60.

GALOIS R G.Variation de la composition lipidque tissulaire au cours de la vetellogenes chez la crevette Penaeus indicus[J]..J Exp Mar Biol Ecol, 1984, 84:155～166.

CHAPELLE S.Lipid eomposition of tissues of marine crustaceans[J].Biochem Syst Ecol, 1977, 5:241～248.

DALL W, CHANDUMPAI A, SMITH D M.The fate of some ¹⁴C-labelled dietary lipids in the tiger prawn Penaeus esculentus[J].Mar Biol, 1993, 115:39～45.

TFSHIMA S KANAZAWA A, KAKUTA Y.Role of dietary phospholipids in the transport of ¹⁴C-cholesterol in the prawn[J].Bull Jap Soc Sci Fish, 1986, 52:719～723.

TEAHIMA S, KANAZAWA A, KAKUTA Y.Role of dietary phospholipids in the transport of ¹⁴C-tripalmitin in the pram[J].Bull Jap Soc Sci Fish, 1986, 52:519～524.

TESHIMA S, KANAZAWA A, HITOSUMATSU K I, et al.Tssue uptake and bioconveraion of isosapentaenoic acid and phosphatidylcholine in prawan, Penaeus and Macrobrachium[J].Comp Biochem Physiol, 1992, 102B:885～890.

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