斑节对虾病原菌胞外产物的致病性研究
Studies on pathogenesis of extracellular products from pathogenetic bacteria in Penaeus monodon Fabrivius
-
摘要: 由养殖濒死病虾体内分离的副溶血弧菌(2019#)、溶藻弧菌(4302#)和嗜水气单胞菌(1003#)培养液中分别提取了胞外产物,测定了酶成分,定量分析了蛋白酶及磷酸酯酶的活性.用细菌胞外产物粗提液对斑节对虾进行致死试验,戊二醛固定致死对虾的肝组织,电镜观察了斑节对虾肝组织的超微病变,初步探讨了三株细菌胞外产物诱发虾病的病理.Abstract: The extracellular products of Vibrio parahaemolyticus,V.Alginolyticus and Aeromonas hydrophila,which were isolated from diseased Penaeus monodon in Tong'an from June to August 1997,are extracted and investigated.Composition of extracellular enzymes from these three bacteria is detected.Among them,protease and phospholiase,including both acidic phospholiase and alkine phospholiase,are chosen to do quantitative analysis on their activities.These three kinds of the above enzymes extracted from cultures of V.Parahaemolyticus are more active than those of the others.Bacterial extracellular products are used to do lethal experiments by injecting to the healthy P.Monodon.Conforming to the result of activity of protease and phospholiase,lethal action of V.Parahaemolyticus' is the strongest and that of V.Alginolyticus' is the weakest.The pathological variations of dying shrimps' livers in the lethal experiments are observed by transmit electron microscope (TEM).Cells of those shrimps' livers show to be destroyed at different extents,for example,nuclear core of some cells disappeared and nuclear membrane are wrecked,mitochondria ave destroyed,even the whole cell is disintegrated.It is showed that not only extracellular products of these three strains are poisonous and could be mortal to shrimps,but also the activities of protease and phospholiase in extracellular products of pathogen are related to toxicity of these three strains.
-
Key words:
- Pathogens /
- extracellular products /
- Penaeus monodon
-
张朝霞,王军,苏永全等.斑节对虾细菌性疾病的病原生物学研究.虾类的健康养殖.北京:海洋出版社,1998.129～138 陆承平.致病性嗜水气单胞菌及其所致鱼病综述.水产学报,1992,16(3):282～287 Lightner D V,Lewis D H.A septicemic bacterial disease syndrome of penaeid shrimp.Marine Fish Review,1976,(37):25～28 沈亚荣,于业绍.副溶血弧菌对文蛤的致病性及其防治.水产学报,1993,17(3):249～252 Ototake M,Wakabayashi H.Characteristics of extracellular products of Flavobacterium sp.,a pathogen of bacterial gill disease.Fish Pathology,1985,(20):167～171 Inamura H.Toxicity of extracellular products of Vibrio anguillarum.Fish Pathology,1987,22:153～158 邱秀宝,李彤,戴 宏,等.嗜冷性海洋微生物蛋白酶的研究.微生物学通报,1991,18(3):138～141 中国科学院微生物研究所细菌分类组.一般细菌常用鉴定方法.北京:科学出版社,1978 Phihpp Gerhardt主编.厦门大学生物系微生物教研室译.普通细菌学方法手册.厦门:厦门大学出版社,1989 谢湘玉.紫外诱变球孢白僵菌选育几丁质酶高产菌株方法的研究.河南师范大学学报(自然科学版).1996,24(3):75～77 北京大学生物系生物化学教研室编.生物化学实验指导.北京:人民卫生出版社,1987 金城,杨寿钧,张树政.嗜热脂肪芽孢杆菌HY-60耐热中性蛋白酶的性质研究.生物化学与生物物理学报,1994,26(4):357～363 陈清西,颜思旭.文昌鱼的碱性磷酸酶的必需基因研究.厦门大学学报(自然科学版),1986,25(3),568～573 陈素丽,陈清西,胡天惠,等.长毛对虾酸性磷酸酶的纯化与性质.厦门大学学报(自然科学版)、1997,36(1):121～125 许兵,纪伟尚,徐怀恕,等.中国对虾病原菌及其致病机理的研究.海洋学报,1993,15(1):98～106 樊美珍,胡锦江、李农昌,等.球孢白僵菌胞外蛋白酶及其与毒力关系的研究.微生物学通报,1994,21(4):202～206 李建南,汪美先,过祥豹.不同血清型和感染来源绿脓杆菌的毒力测定.中华微生物学和免疫学杂志,1990,10(6):357～359 Kimi R M,Iwanaga S.Structure-function relationships of phospholipase I.Prediction of presynaptic neurotoxicity.Toxicon,1986,(24):527 王军,张朝霞,苏永全,等.斑节对虾病原菌的回归感染试验.虾类的健康养殖,北京:海洋出版社,1998.139～145 -
点击查看大图
计量
- 文章访问数: 1064
- HTML全文浏览量: 16
- PDF下载量: 986
- 被引次数: 0
下载:
