几种神经活性物质对海湾扇贝幼虫变态诱导作用的研究
Study on induction of metamorphosis in larvae of bay scallop, Argopecten irradians, by some neuroactive compounds
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摘要: 本文研究了儿茶酚胺类和金属离子对海湾扇贝幼虫变态的诱导作用,结果显示;外加10-4-10-6mol/dm3的肾上腺素,10-5mol/dm3的去甲肾上腺素和L-多巴具有十分显着的诱导能力;外加10-15mmol/dm3K+和Ca2+处理幼虫一段时间后,对其变态有明显促进作用。实验研究了最适浓度下肾上腺素、L-多巴和KCl不同处理时间对幼虫变态的影响,表明幼虫对肾上腺素和L-多巴表现出很高的敏感性,而对KCl则表现出对时间和剂量较强的依赖性。Abstract: The larvae of bay scallop Argopecten irradians were treated with catecholamines and metal canons, to test the ability of these compounds to induce metamorphosis. Epinephrine (10-4~10-6 mol/dm3),norepinephrine(10-5 mol/dm3)and L-DOPA(10-5 mol/dm3)could increase metamorphosis significantly; at long duration exposure to excess KCl and CaCl2,the metamorphosis of larvae was increased. When the larvae were induced with the optimum concentration of epinephrine, L-DOPA and KCl in a series time, they exhibited high sensitivity to epinephrine and L-DOPA, but the efficiency of K+ depended on time and dose. These suggest that epinephrine and L-DOPA appear to be via receptor-mediated pathway to trigger the process of metamorphosis; the elevated external concentration of K+ is to cause the depolarization of excitable cells and then enhance the larvae to metamorphose. The results of our study can offer an useful technique for inducing metamorphosis in spats commercial rearing of molluscs.
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Key words:
- Bay scallop /
- larvae /
- metamorphosis /
- chemical inducement
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